Inhibitory effects of Polygonum cuspidatum water extract (PCWE) and its component rasveratrol on acyl-coenzyme A–cholesterol acyltransferase activity for cholesteryl ester synthesis in HepG2 cells

2004 ◽  
Vol 40 (6) ◽  
pp. 279-284 ◽  
Author(s):  
Cheol-Soo Park ◽  
Young-Choon Lee ◽  
Jong-Dae Kim ◽  
Hyung-Min Kim ◽  
Cheorl-Ho Kim
Keyword(s):  
Cholesteryl Ester ◽  
Hepg2 Cells ◽  
Coenzyme A ◽  
Cholesterol Acyltransferase ◽  
Water Extract ◽  
Polygonum Cuspidatum ◽  
Ester Synthesis ◽  
Inhibitory Effects ◽  
Acyltransferase Activity ◽  
Acyl Coenzyme A

Acyl-Coenzyme A—Cholesterol Acyltransferase Activity in Human Liver

1979 ◽  
Vol 56 (4) ◽  
pp. 373-375 ◽  
Author(s):  
S. Balasubramaniam ◽  
K. A. Mitropoulos ◽  
N. B. Myant ◽  
M. Mancini ◽  
A. Postiglione
Keyword(s):  
Liver Enzyme ◽  
Rat Liver ◽  
Human Liver ◽  
Microsomal Fraction ◽  
Coenzyme A ◽  
Cholesterol Acyltransferase ◽  
Liver Microsomes ◽  
Cholesteryl Oleate ◽  
Acyltransferase Activity ◽  
Acyl Coenzyme A

1. In the presence of CoA and ATP, human liver microsomes catalyse the incorporation of [14C]oleate or [14C]cholesterol into cholesteryl oleate, thus demonstrating the presence of acyl-coenzyme A-cholesterol acyltransferase (cholesterol acyltransferase) in human liver. 2. The enzyme has properties similar to those of rat liver enzyme and with both the concentration of endogenous cholesterol in the microsomal fraction is adequate to support a constant initial rate of esterification. However, unlike the rat liver enzyme, the human cholesterol acyltransferase does not efficiently utilize added cholesterol as substrate. 3. The activity of cholesterol acyltransferase in human liver was 25% of that measured in rat liver under similar conditions of assay.

scholarly journals Phosphatidylcholine increases the secretion of triacylglycerol-rich lipoproteins by CaCo-2 cells

1996 ◽  
Vol 314 (2) ◽  
pp. 569-575 ◽  
Author(s):  
Satya N. MATHUR ◽  
Ella BORN ◽  
Shubha MURTHY ◽  
F. Jeffrey FIELD
Keyword(s):  
Cholesteryl Ester ◽  
Secretory Pathway ◽  
Cholesterol Acyltransferase ◽  
Lipid Synthesis ◽  
Brefeldin A ◽  
Mrna Levels ◽  
Ester Synthesis ◽  
Acyltransferase Activity ◽  
Apob Mrna ◽  
Triacylglycerol Synthesis

The regulation of lipid synthesis and secretion by phosphatidylcholine was investigated in CaCo-2 cells grown on semipermeable filters. In cells incubated with 1 mM taurocholate and 100–500 μM phosphatidylcholine, cholesteryl ester synthesis was decreased, triacylglycerol synthesis was increased modestly, whereas phospholipid synthesis was unaffected. Acyl-CoA–cholesterol acyltransferase activity was decreased secondary to a decrease in the substrate (cholesterol) supply. The basolateral secretion of newly synthesized triacylglycerol and triacylglycerol mass was significantly increased by phosphatidylcholine, whereas cellular triacylglycerol mass decreased. This effect was not specific for phosphatidylcholine as phosphatidylethanolamine and phosphatidylserine also increased the secretion of newly synthesized triacylglycerols. Dioleoylphosphatidylcholine was as effective as egg phosphatidylcholine in increasing triacylglycerol transport. Dipalmitoylphosphatidylcholine, in contrast, was without effect. Phosphatidylcholine also increased the basolateral secretion of apolipoprotein B (apoB) mass without altering apoB mRNA levels. Disruption of the Golgi apparatus by monensin or brefeldin A prevented the increase in apoB secretion by phosphatidylcholine. Compared with microsomes prepared from control cells, those from cells incubated with phosphatidylcholine contained more newly synthesized apoB. The percentage of new synthesized apoB isolated from the lumen of microsomes (as an estimate of apoB destined for secretion), however, was similar in the two preparations. Thus in CaCo-2 cells incubated with phosphatidylcholine, the transport of apoB and triacylglycerols is increased whereas cholesteryl ester synthesis and secretion are decreased. A normally functioning secretory pathway is required for phosphatidylcholine to increase triacylglycerol-rich lipoprotein secretion.

scholarly journals Studies on acyl-coenzyme A: cholesterol acyltransferase activity in human liver microsomes.

1989 ◽  
Vol 30 (5) ◽  
pp. 739-746
Author(s):  
K Einarsson ◽  
L Benthin ◽  
S Ewerth ◽  
G Hellers ◽  
D Ståhlberg ◽  
...  
Keyword(s):  
Human Liver ◽  
Coenzyme A ◽  
Cholesterol Acyltransferase ◽  
Liver Microsomes ◽  
Human Liver Microsomes ◽  
Acyltransferase Activity ◽  
Acyl Coenzyme A

Conjugated and non-conjugated octadecaenoic acids affect differently intestinal acyl coenzyme A: Cholesterol acyltransferase activity

2008 ◽  
Vol 198 (1) ◽  
pp. 85-93 ◽  
Author(s):  
Cheuk Kai Lam ◽  
Jingnan Chen ◽  
Ying Cao ◽  
Lin Yang ◽  
Yin Mei Wong ◽  
...  
Keyword(s):  
Coenzyme A ◽  
Cholesterol Acyltransferase ◽  
Acyltransferase Activity ◽  
Acyl Coenzyme A
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