scholarly journals Modulating immunogenic properties of HIV-1 gp41 membrane-proximal external region by destabilizing six-helix bundle structure

Virology ◽  
2016 ◽  
Vol 490 ◽  
pp. 17-26 ◽  
Author(s):  
Saikat Banerjee ◽  
Heliang Shi ◽  
Habtom H. Habte ◽  
Yali Qin ◽  
Michael W. Cho
Keyword(s):  
External Region ◽  
Membrane Proximal External Region ◽  
Helix Bundle ◽  
Immunogenic Properties ◽  
Hiv 1 ◽  
Bundle Structure

scholarly journals Destabilization of Highly Rigid Bilayers Enriched in Cholesterol by the Membrane-Proximal External Region of HIV-1 gp41

2011 ◽  
Vol 100 (3) ◽  
pp. 634a
Author(s):  
Beatriz Apellaniz ◽  
Andrey Ivankin ◽  
David Gidalevitz ◽  
Jose L. Nieva
Keyword(s):  
External Region ◽  
Membrane Proximal External Region ◽  
Hiv 1

The interaction between the membrane-proximal external region and the N-trimer region of HIV-1 gp41: Involvement in viral fusion

2009 ◽  
Vol 54 (10) ◽  
pp. 1707-1712
Author(s):  
Jing Li ◽  
Lu Lu ◽  
Fan Wu ◽  
Xi Chen ◽  
Ben Niu ◽  
...  
Keyword(s):  
Viral Fusion ◽  
External Region ◽  
Membrane Proximal External Region ◽  
Hiv 1

scholarly journals Anti-V3/Glycan and Anti-MPER Neutralizing Antibodies, but Not Anti-V2/Glycan Site Antibodies, Are Strongly Associated with Greater Anti-HIV-1 Neutralization Breadth and Potency

2015 ◽  
Vol 89 (10) ◽  
pp. 5264-5275 ◽  
Author(s):  
Rajesh Abraham Jacob ◽  
Thandeka Moyo ◽  
Michael Schomaker ◽  
Fatima Abrahams ◽  
Berta Grau Pujol ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Prediction Interval ◽  
Vaccine Design ◽  
Broadly Neutralizing Antibodies ◽  
External Region ◽  
Membrane Proximal External Region ◽  
Site Recognition ◽  
Anti Hiv ◽  
Glycan Site ◽  
Hiv 1

ABSTRACTThe membrane-proximal external region (MPER), the V2/glycan site (initially defined by PG9 and PG16 antibodies), and the V3/glycans (initially defined by PGT121–128 antibodies) are targets of broadly neutralizing antibodies and potential targets for anti-HIV-1 antibody-based vaccines. Recent evidence shows that antibodies with moderate neutralization breadth are frequently attainable, with 50% of sera from chronically infected individuals neutralizing ≥50% of a large, diverse set of viruses. Nonetheless, there is little systematic information addressing which specificities are preferentially targeted among such commonly found, moderately broadly neutralizing sera. We explored associations between neutralization breadth and potency and the presence of neutralizing antibodies targeting the MPER, V2/glycan site, and V3/glycans in sera from 177 antiretroviral-naive HIV-1-infected (>1 year) individuals. Recognition of both MPER and V3/glycans was associated with increased breadth and potency. MPER-recognizing sera neutralized 4.62 more panel viruses than MPER-negative sera (95% prediction interval [95% PI], 4.41 to 5.20), and V3/glycan-recognizing sera neutralized 3.24 more panel viruses than V3/glycan-negative sera (95% PI, 3.15 to 3.52). In contrast, V2/glycan site-recognizing sera neutralized only 0.38 more panel viruses (95% PI, 0.20 to 0.45) than V2/glycan site-negative sera and no association between V2/glycan site recognition and breadth or potency was observed. Despite autoreactivity of many neutralizing antibodies recognizing MPER and V3/glycans, antibodies to these sites are major contributors to neutralization breadth and potency in this cohort. It may therefore be appropriate to focus on developing immunogens based upon the MPER and V3/glycans.IMPORTANCEPrevious candidate HIV vaccines have failed either to induce wide-coverage neutralizing antibodies or to substantially protect vaccinees. Therefore, current efforts focus on novel approaches never before successfully used in vaccine design, including modeling epitopes. Candidate immunogen models identified by broadly neutralizing antibodies include the membrane-proximal external region (MPER), V3/glycans, and the V2/glycan site. Autoreactivity and polyreactivity of anti-MPER and anti-V3/glycan antibodies are thought to pose both direct and indirect barriers to achieving neutralization breadth. We found that antibodies to the MPER and the V3/glycans contribute substantially to neutralization breadth and potency. In contrast, antibodies to the V2/glycan site were not associated with neutralization breadth/potency. This suggests that the autoreactivity effect is not critical and that the MPER and the V3/glycans should remain high-priority vaccine candidates. The V2/glycan site result is surprising because broadly neutralizing antibodies to this site have been repeatedly observed. Vaccine design priorities should shift toward the MPER and V3/glycans.

Exposure of the Membrane-Proximal External Region of HIV-1 gp41 in the Course of HIV-1 Envelope Glycoprotein-Mediated Fusion†

Biochemistry ◽  
2007 ◽  
Vol 46 (5) ◽  
pp. 1398-1401 ◽  
Author(s):  
Antony S. Dimitrov ◽  
Amy Jacobs ◽  
Catherine M. Finnegan ◽  
Gabriela Stiegler ◽  
Hermann Katinger ◽  
...  
Keyword(s):  
Envelope Glycoprotein ◽  
External Region ◽  
Membrane Proximal External Region ◽  
Hiv 1

scholarly journals HIV-1 gp41 Core with Exposed Membrane-Proximal External Region Inducing Broad HIV-1 Neutralizing Antibodies

PLoS ONE ◽  
2011 ◽  
Vol 6 (3) ◽  
pp. e18233 ◽  
Author(s):  
Ji Wang ◽  
Pei Tong ◽  
Lu Lu ◽  
Leilei Zhou ◽  
Liling Xu ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
External Region ◽  
Membrane Proximal External Region ◽  
Hiv 1

scholarly journals N-terminal residues of an HIV-1 gp41 membrane-proximal external region antigen influence broadly neutralizing 2F5-like antibodies

2015 ◽  
Vol 30 (6) ◽  
pp. 449-456 ◽  
Author(s):  
Dezhi Li ◽  
Jie Liu ◽  
Li Zhang ◽  
Tianshu Xu ◽  
Junheng Chen ◽  
...  
Keyword(s):  
External Region ◽  
Membrane Proximal External Region ◽  
Hiv 1

scholarly journals Bovine papillomavirus-like particles presenting conserved epitopes from membrane-proximal external region of HIV-1 gp41 induced mucosal and systemic antibodies

Vaccine ◽  
2013 ◽  
Vol 31 (46) ◽  
pp. 5422-5429 ◽  
Author(s):  
Yougang Zhai ◽  
Zhenyu Zhong ◽  
Mohammadreza Zariffard ◽  
Gregory T. Spear ◽  
Liang Qiao
Keyword(s):  
Bovine Papillomavirus ◽  
External Region ◽  
Membrane Proximal External Region ◽  
Hiv 1
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