scholarly journals Characterization of a spliced exon product of herpes simplex type-1 latency-associated transcript in productively infected cells

Virology ◽  
2006 ◽  
Vol 356 (1-2) ◽  
pp. 106-114 ◽  
Author(s):  
Wen Kang ◽  
Ruma Mukerjee ◽  
Jared J. Gartner ◽  
Artemis G. Hatzigeorgiou ◽  
Rozanne M. Sandri-Goldin ◽  
...  
Keyword(s):  
Herpes Simplex ◽  
Herpes Simplex Type ◽  
Infected Cells

scholarly journals Characterization of Herpes Simplex Viruses Selected in Culture for Resistance to Penciclovir or Acyclovir

2001 ◽  
Vol 75 (4) ◽  
pp. 1761-1769 ◽  
Author(s):  
Robert T. Sarisky ◽  
Matthew R. Quail ◽  
Philip E. Clark ◽  
Tammy T. Nguyen ◽  
Wendy S. Halsey ◽  
...  
Keyword(s):  
Herpes Simplex ◽  
Sequence Similarity ◽  
Cross Resistance ◽  
Phenotypic Analysis ◽  
Herpes Simplex Viruses ◽  
Infected Cells ◽  
Simplex Virus ◽  
Hsv 1

ABSTRACT Penciclovir (PCV), an antiherpesvirus agent in the same class as acyclovir (ACV), is phosphorylated in herpes simplex virus (HSV)-infected cells by the viral thymidine kinase (TK). Resistance to ACV has been mapped to mutations within either the TK or the DNA polymerase gene. An identical activation pathway, the similarity in mode of action, and the invariant cross-resistance of TK-negative mutants argue that the mechanisms of resistance to PCV and ACV are likely to be analogous. A total of 48 HSV type 1 (HSV-1) and HSV-2 isolates were selected after passage in the presence of increasing concentrations of PCV or ACV in MRC-5 cells. Phenotypic analysis suggested these isolates were deficient in TK activity. Moreover, sequencing of the TK genes from ACV-selected mutants identified two homopolymeric G-C nucleotide stretches as putative hot spots, thereby confirming previous reports examining Acvr clinical isolates. Surprisingly, mutations identified in PCV-selected mutants were generally not in these regions but distributed throughout the TK gene and at similar frequencies of occurrence within A-T or G-C nucleotides, regardless of virus type. Furthermore, HSV-1 isolates selected in the presence of ACV commonly included frameshift mutations, while PCV-selected HSV-1 mutants contained mostly nonconservative amino acid changes. Data from this panel of laboratory isolates show that Pcvr mutants share cross-resistance and only limited sequence similarity with HSV mutants identified following ACV selection. Subtle differences between PCV and ACV in the interaction with viral TK or polymerase may account for the different spectra of genotypes observed for the two sets of mutants.

Nucleoside pools of acyclovir-treated herpes simplex type 1 infected cells

1985 ◽  
Vol 5 (2) ◽  
pp. 75-81 ◽  
Author(s):  
Johan Harmenberg ◽  
Gunnar Abele ◽  
Britta Wahren
Keyword(s):  
Herpes Simplex ◽  
Herpes Simplex Type ◽  
Infected Cells

Fixed herpes simplex type 1-infected cells as antigen for in vitro lymphocyte proliferation

1985 ◽  
Vol 76 (2) ◽  
pp. 239-246 ◽  
Author(s):  
Sytske Welling-Wester ◽  
Bertha A. Huitema ◽  
Jan B. Wilterdink
Keyword(s):  
Herpes Simplex ◽  
Lymphocyte Proliferation ◽  
Herpes Simplex Type ◽  
Infected Cells

Construction and characterization of a recombinant plasmid encoding the gene for the thymidine kinase of herpes simplex type 1 virus

Gene ◽  
1979 ◽  
Vol 7 (3-4) ◽  
pp. 335-342 ◽  
Author(s):  
Lynn W. Enquist ◽  
George F. Vande Woude ◽  
Michael Wagner ◽  
James R. Smiley ◽  
William C. Summers
Keyword(s):  
Herpes Simplex ◽  
Thymidine Kinase ◽  
Recombinant Plasmid ◽  
Herpes Simplex Type
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