Surface-enhanced Raman spectroscopy of molecules adsorbed on silver nanoparticles dispersed an agarose gel and their adsorption isotherms

2016 ◽  
Vol 86 ◽  
pp. 290-301 ◽  
Author(s):  
Karolyne V. de Oliveira ◽  
Joel C. Rubim
Author(s):  
Scott G. Harroun ◽  
Yaoting Zhang ◽  
Tzu-Heng Chen ◽  
Huan-Tsung Chang ◽  
Alexis Vallée-Bélisle

For simulation of SERS on silver nanoparticles, Ag2O can provide a more accurate result than standard model surfaces such as Ag+, Ag, Ag4+ and Ag4.


Nanoscale ◽  
2022 ◽  
Author(s):  
Da Li ◽  
Philippe Nizard ◽  
Delphine Onidas ◽  
Aazdine Lamouri ◽  
Jean Pinson ◽  
...  

The surface functionalization of silver nanoparticles (NPs) by Raman reporters has stimulated a wide interest in recent years for the design of Surface-Enhanced Raman Spectroscopy (SERS) labels. However, silver NPs...


2019 ◽  
Vol 50 (12) ◽  
pp. 1849-1860
Author(s):  
Cristina M. Muntean ◽  
Nicoleta E. Dina ◽  
Maria Coroş ◽  
Nicoleta Toşa ◽  
Alexandru I. Turza ◽  
...  

Nanoscale ◽  
2018 ◽  
Vol 10 (13) ◽  
pp. 5897-5905 ◽  
Author(s):  
Zhen Li ◽  
Shouzhen Jiang ◽  
Yanyan Huo ◽  
Tingyin Ning ◽  
Aihua Liu ◽  
...  

We explore the variation of SERS performance towards two different Ag NP layers using multilayer GO as a spacer.


Nanomaterials ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 1561 ◽  
Author(s):  
Maria João Oliveira ◽  
Miguel P. de Almeida ◽  
Daniela Nunes ◽  
Elvira Fortunato ◽  
Rodrigo Martins ◽  
...  

Immunoassays using Surface-Enhanced Raman Spectroscopy are especially interesting on account not only of their increased sensitivity, but also due to its easy translation to point-of-care formats. The bases for these assays are bioconjugates of polyclonal antibodies and anisotropic gold nanoparticles functionalized with a Raman reporter. These bioconjugates, once loaded with the antigen analyte, can react on a sandwich format with the same antibodies immobilized on a surface. This surface can then be used for detection, on a microfluidics or immunochromatographic platform. Here, we have assembled bioconjugates of gold nanostars functionalized with 4-mercaptobenzoic acid, and anti-horseradish peroxidase antibodies. The assembly was by simple incubation, and agarose gel electrophoresis determined a high gold nanostar to antibody binding constant. The functionality of the bioconjugates is easy to determine since the respective antigen presents peroxidase enzymatic activity. Furthermore, the chosen antibody is a generic immunoglobulin G (IgG) antibody, opening the application of these principles to other antibody-antigen systems. Surface-Enhanced Raman Spectroscopy analysis of these bioconjugates indicated antigen detection down to 50 µU of peroxidase activity. All steps of conjugation were fully characterized by ultraviolet-visible spectroscopy, dynamic light scattering, ζ -Potential, scanning electron microscopy, and agarose gel electrophoresis. Based on the latter technique, a proof-of-concept was established for the proposed immunoassay.


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