Sampling and repeatability of radiometric faecal culture in bovine Johne's disease

2007 ◽  
Vol 119 (2-4) ◽  
pp. 184-193 ◽  
Author(s):  
Graeme J. Eamens ◽  
Mark J. Turner ◽  
Richard J. Whittington
Keyword(s):  
Johne's Disease ◽  
Johne’S Disease ◽  
Faecal Culture

Johne’s disease: a successful eradication programme in a dairy goat herd

2018 ◽  
Vol 182 (17) ◽  
pp. 483-483 ◽  
Author(s):  
William G Gavin ◽  
Catherine A Porter ◽  
Nathan Hawkins ◽  
Michael J Schofield ◽  
John M Pollock
Keyword(s):  
Johne's Disease ◽  
Johne’S Disease ◽  
Dairy Goat ◽  
Dairy Goats ◽  
Eradication Programme ◽  
Agar Gel ◽  
Serological Screening ◽  
Surveillance Programme ◽  
Oral Transmission ◽  
Faecal Culture

This retrospective analysis and report describes the successful eradication and posteradication surveillance programme for Johne’s disease (Mycobacterium avium subspecies paratuberculosis (MAP)) in a closed herd of dairy goats. In 1994, MAP’s presence in the goat herd was first suspected through individual annual serological screening and then subsequently confirmed through faecal culture and histopathology in 1997 when implementation of a more aggressive programme of testing and eradication of the diseased animals began. This programme included frequent serological screening of all adult goats using ELISA and agar gel immunodiffusion assays. Faecal cultures for bacteria were performed on suspect or positive animals and for all goats found dead or euthanased, and tissues were submitted for histopathology and acid-fast staining. Additional disease eradication measures included maintaining a closed herd and minimising faecal-oral transmission of MAP. Following a more aggressive testing regimen and euthanasia of goats with positive faecal culture, the herd was first considered free of MAP in 2003 and has remained free to the present day.

scholarly journals Within-herd prevalence thresholds for the detection of Mycobacterium avium subspecies paratuberculosis-positive dairy herds using boot swabs and liquid manure samples

2015 ◽  
Vol 144 (2) ◽  
pp. 413-424 ◽  
Author(s):  
K. DONAT ◽  
N. HAHN ◽  
T. EISENBERG ◽  
K. SCHLEZ ◽  
H. KÖHLER ◽  
...  
Keyword(s):  
Mycobacterium Avium ◽  
Mycobacterium Avium Subspecies Paratuberculosis ◽  
Threshold Level ◽  
Johne's Disease ◽  
Probability Of Detection ◽  
Johne’S Disease ◽  
Dairy Herds ◽  
Liquid Manure ◽  
Herd Prevalence ◽  
Faecal Culture

SUMMARYThe control of Johne's disease requires the identification of Mycobacterium avium ssp. paratuberculosis (MAP)-positive herds. Boot swabs and liquid manure samples have been suggested as an easy-to-use alternative to sampling individual animals in order to diagnose subclinical Johne's disease at the herd level, but there is a need to evaluate performance of this approach in the field. Using a logistic regression model, this study aimed to calculate the threshold level of the apparent within-herd prevalence as determined by individual faecal culture, thus allowing the detection of whether a herd is MAP positive. A total of 77 boot swabs and 75 liquid manure samples were taken from 19 certified negative and 58 positive dairy herds. Faecal culture, three different polymerase chain reaction (PCR) methods and the combination of faecal culture with PCR were applied in order to detect MAP. For 50% probability of detection, a within-herd prevalence threshold of 1·5% was calculated for testing both matrices simultaneously by faecal culture and PCR, with the threshold increased to 4·0% for 90% probability of detection. The results encourage the use of boot swabs or liquid manure samples, or a combination both, for identifying MAP-positive herds and, to a certain extent, for monitoring certified Johne's disease-negative cattle herds.

Changes to Johne's disease testing regime

2021 ◽  
Vol 188 (11) ◽  
pp. 438-438
Author(s):  
Keith Cutler ◽  
Bridget Taylor ◽  
Gareth Boyes ◽  
Darren Todd ◽  
Abi Reader
Keyword(s):  
Johne's Disease ◽  
Johne’S Disease ◽  
Disease Testing

Factors influencing the effectiveness of Gudair® vaccine for controlling Johne’s disease in sheep flocks in Australia

2021 ◽  
pp. 105394
Author(s):  
Navneet K. Dhand ◽  
Karren M. Plain ◽  
Alexandra C. Green ◽  
Esteban Martinez ◽  
Jeff Eppleston ◽  
...  
Keyword(s):  
Johne's Disease ◽  
Johne’S Disease ◽  
Factors Influencing
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