Effects of pre-culture holding time and temperature on interferon-γ responses in whole blood cultures from Mycobacterium bovis-infected cattle

2007 ◽  
Vol 119 (2-4) ◽  
pp. 277-282 ◽  
Author(s):  
W.R. Waters ◽  
B.J. Nonnecke ◽  
S.C. Olsen ◽  
M.V. Palmer
Keyword(s):  
Mycobacterium Bovis ◽  
Whole Blood ◽  
Interferon Γ ◽  
Blood Cultures ◽  
Holding Time ◽  
Infected Cattle

Effects of culture conditions and tuberculin source on interferon-γ production in whole blood cultures from Mycobacterium bovis infected cattle

2009 ◽  
Vol 128 (1-3) ◽  
pp. 230-231
Author(s):  
Irene Schiller ◽  
Ray Waters ◽  
Martin Vordermeier ◽  
Mitchell Palmer ◽  
Teklu Egnuni ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Whole Blood ◽  
Interferon Γ ◽  
Culture Conditions ◽  
Blood Cultures ◽  
Infected Cattle

scholarly journals Interleukin‐18 Enhances Lipopolysaccharide‐Induced Interferon‐γ Production in Human Whole Blood Cultures

1998 ◽  
Vol 178 (6) ◽  
pp. 1830-1834 ◽  
Author(s):  
Adrian J. Puren ◽  
Peter Razeghi ◽  
Giamila Fantuzzi ◽  
Charles A. Dinarello
Keyword(s):  
Whole Blood ◽  
Interferon Γ ◽  
Blood Cultures ◽  
Interleukin 18 ◽  
Human Whole Blood

scholarly journals Stimulation of Bovine Whole-Blood Samples Cultured in Media Supplemented with Recombinant Interleukin-7 (IL-7) and IL-12 Extends the Life Span of the Gamma Interferon Assay To Detect Mycobacterium bovis-Infected Cattle

2016 ◽  
Vol 54 (9) ◽  
pp. 2315-2320 ◽  
Author(s):  
E. Gerace ◽  
P. Pasquali ◽  
B. Oesch ◽  
M. Falduto ◽  
F. Mandanici ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Whole Blood ◽  
Gamma Interferon ◽  
Infected Cattle ◽  
Blood Samples ◽  
Content Type ◽  
Rpmi Medium ◽  
Interleukin 7 ◽  
Ifn Γ ◽  
Whole Blood Samples

The gamma interferon (IFN-γ) assay is widely used to measure cell-mediated immune (CMI) response for the early detection of tuberculosis infection. Processing whole-blood samples for CMI-based diagnostics is time sensitive and usually must occur within 8 h of collection to ensure optimal assay performance. In this study, we developed and tested a modified protocol, in which whole-blood samples fromMycobacterium bovis-infected cattle were diluted 1:1 in RPMI medium containing 0.3% fetal bovine serum (FBS) added or not to recombinant mouse interleukin-7 (rmIL-7) or rmIL-12, alone or in combination, and stored at 4°C. At 3 and 6 days postcollection, the diluted blood samples were adjusted to 10% FBS, dispensed into culture trays, stimulated with a bovine purified protein derivative fromM. bovis, and incubated at 37°C in 5% CO2in air. Plasma was removed and assayed for an IFN-γ response using bovine IFN-γ enzyme-linked immunosorbent assay (ELISA) (Bovigam). The results were then compared with those obtained from the conventional procedure. The IFN-γ responses of the samples stored up to 6 days postcollection in the supplemented RPMI medium were similar to those observed in the samples processed within 8 h after sampling, indicating that lymphocyte vitality and response were preserved. The addition of rmIL-7 and rmIL-12, alone or in combination, to culture medium can enhance lymphocyte survival and thus extends the time limit within which the IFN-γ assay can be applied as a diagnostic tool in bovine tuberculosis surveillance and eradication.

IL-10 suppression of IFN-γ responses in tuberculin-stimulated whole blood from Mycobacterium bovis infected cattle

2017 ◽  
Vol 189 ◽  
pp. 36-42 ◽  
Author(s):  
Michael P. Sheridan ◽  
John A. Browne ◽  
Mairead B. Doyle ◽  
Tara Fitzsimons ◽  
Kevina McGill ◽  
...  
Keyword(s):  
Mycobacterium Bovis ◽  
Whole Blood ◽  
Infected Cattle ◽  
Ifn Γ

scholarly journals Severity of bovine tuberculosis is associated with innate immune-biased transcriptional signatures of whole blood in early weeks after experimental Mycobacterium bovis infection

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0239938
Author(s):  
Jayne E. Wiarda ◽  
Paola M. Boggiatto ◽  
Darrell O. Bayles ◽  
W. Ray Waters ◽  
Tyler C. Thacker ◽  
...  
Keyword(s):  
Disease Severity ◽  
Mycobacterium Bovis ◽  
Whole Blood ◽  
Post Infection ◽  
Bovine Tuberculosis ◽  
Severe Disease ◽  
Clinical Status ◽  
Cell Types ◽  
Innate Immune ◽  
Infected Cattle

Mycobacterium bovis, the causative agent of bovine tuberculosis, is a pathogen that impacts both animal and human health. Consequently, there is a need to improve understanding of disease dynamics, identification of infected animals, and characterization of the basis of immune protection. This study assessed the transcriptional changes occurring in cattle during the early weeks following a M. bovis infection. RNA-seq analysis of whole blood-cell transcriptomes revealed two distinct transcriptional clusters of infected cattle at both 4- and 10-weeks post-infection that correlated with disease severity. Cattle exhibiting more severe disease were transcriptionally divergent from uninfected animals. At 4-weeks post-infection, 25 genes had commonly increased expression in infected cattle compared to uninfected cattle regardless of disease severity. Ten weeks post-infection, differential gene expression was only observed when severely-affected cattle were compared to uninfected cattle. This indicates a transcriptional divergence based on clinical status following infection. In cattle with more severe disease, biological processes and cell type enrichment analyses revealed overrepresentation of innate immune-related processes and cell types in infected animals. Collectively, our findings demonstrate two distinct transcriptional profiles occur in cattle following M. bovis infection, which correlate to clinical status.

Supplemental fish oil does not alter immune competence or the pathophysiological response to an intramammary infusion of endotoxin in peri-partum multiparous Holstein cows

2009 ◽  
Vol 76 (2) ◽  
pp. 165-172 ◽  
Author(s):  
Michael A Ballou ◽  
Rodrigo C Gomes ◽  
Edward J DePeters
Keyword(s):  
Antimicrobial Activity ◽  
Fish Oil ◽  
Acute Phase ◽  
Whole Blood ◽  
Acute Phase Response ◽  
Post Partum ◽  
Interferon Γ ◽  
Phase Response ◽  
Holstein Cows ◽  
Immune Competence

The objective was to determine the effects of supplementing the diet with fish oil during the peri-partum period on the immune competence and the pathophysiological response to a lipopolysaccharide-induced mastitis challenge. Multiparous Holstein cows (n=30) were completely randomized to one of two treatments at 3 weeks pre-partum. Treatments differed only in the source of supplemental lipid and included either Energy Booster® or fish oil. Treatment diets were fed from −21 d relative to expected date of parturition until 10 d post partum. Treatments were fed as a bolus prior to the a.m. feeding. The dose of lipid during the pre-partum period was 250 g/d, whereas the amount of lipid supplemented post partum was adjusted to the level of intake, approximately 0·92% of the previous day's dry matter intake. Ex-vivo analyses of immune competence were measured including the antimicrobial activity of whole blood against Escherichia coli, Salmonella typhimurium and Candida albicans as well as the production of interferon-γ by peripheral blood mononuclear cultures. At 7 days in milk cows were infused with 100 μg of Esch. coli lipopolysaccharide into one rear quarter. Supplementing fish oil increased plasma concentrations of eicosapentaenoic and docosahexaenoic acids, but had no affect on the proportions of arachidonic acid at calving. Fish oil did not influence the production of interferon-γ or the antimicrobial activity of whole blood against any of the microorganisms. Furthermore, fish oil had no ameliorative effect on either the local or the systemic acute phase response following an intramammary lipopolysaccharide challenge in early lactating Holstein cows. Supplementing fish oil in the diet of peri-partum cows will not protect them from deleterious effects of an excessive acute phase response.

Serial blood cultures from canine stored whole blood held at room temperature for 24 h

2009 ◽  
Vol 18 (3) ◽  
pp. 279-281 ◽  
Author(s):  
J. S. Beymer ◽  
E. Rudloff ◽  
R. Kirby ◽  
T. J. Novicki ◽  
F. M. Moore
Keyword(s):  
Whole Blood ◽  
Room Temperature ◽  
Blood Cultures ◽  
Serial Blood
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