In vitro effects of soluble epoxide hydrolase inhibition on chondrocyte apoptosis and sensitization of peripheral sensory neurons

2018 ◽  
Vol 45 (6) ◽  
pp. 885.e6
Author(s):  
Alonso Guedes ◽  
Troy Trumble ◽  
Laura Tucker ◽  
Caroline Baldo ◽  
Erin Wendt-Hornickle ◽  
...  
Keyword(s):  
Sensory Neurons ◽  
Epoxide Hydrolase ◽  
Soluble Epoxide Hydrolase ◽  
Chondrocyte Apoptosis ◽  
Peripheral Sensory Neurons ◽  
In Vitro Effects ◽  
Peripheral Sensory

scholarly journals Regulation of κ-Opioid Receptor Signaling in Peripheral Sensory Neurons In Vitro and In Vivo

2011 ◽  
Vol 338 (1) ◽  
pp. 92-99 ◽  
Author(s):  
Kelly A. Berg ◽  
Matthew P. Rowan ◽  
Teresa A. Sanchez ◽  
Michelle Silva ◽  
Amol M. Patwardhan ◽  
...  
Keyword(s):  
Opioid Receptor ◽  
Sensory Neurons ◽  
Receptor Signaling ◽  
Peripheral Sensory Neurons ◽  
Peripheral Sensory

scholarly journals Kappa opioid receptor (KOR) signaling on peripheral sensory neurons in vitro and in vivo

2011 ◽  
Vol 25 (S1) ◽  
Author(s):  
Matthew Rowan ◽  
Teresa Sanchez ◽  
Yamille Silva ◽  
Blaine McGuire ◽  
William Clarke ◽  
...  
Keyword(s):  
Opioid Receptor ◽  
Sensory Neurons ◽  
Kappa Opioid Receptor ◽  
Kappa Opioid ◽  
Peripheral Sensory Neurons ◽  
Peripheral Sensory

Soluble Epoxide Hydrolase as an Important Player in Intestinal Cell Differentiation

2020 ◽  
Vol 209 (4-6) ◽  
pp. 177-188
Author(s):  
Katerina Cizkova ◽  
Katerina Koubova ◽  
Tereza Foltynkova ◽  
Jana Jiravova ◽  
Zdenek Tauber
Keyword(s):  
Cell Differentiation ◽  
Colorectal Carcinoma ◽  
Cell Lines ◽  
Epoxide Hydrolase ◽  
Soluble Epoxide Hydrolase ◽  
Intestinal Cell ◽  
Caco2 Cells ◽  
Important Player ◽  
Ht 29

There is growing evidence that soluble epoxide hydrolase (sEH) may play a role in cell differentiation. sEH metabolizes biologically highly active and generally cytoprotective epoxyeicosatrienoic acids (EETs), generated from arachidonic acid metabolism by CYP epoxygenases (CYP2C and CYP2J subfamilies), to less active corresponding diols. We investigated the effect of sEH inhibitor (TPPU) on the expression of villin, CYP2C8, CYP2C9, CYP2J2, and sEH in undifferentiated and in vitro differentiated HT-29 and Caco2 cell lines. The administration of 10 μM TPPU on differentiated HT-29 and Caco2 cells resulted in a significant decrease in expression of villin, a marker for intestinal cell differentiation. It was accompanied by a disruption of the brush border when microvilli appeared sparse and short in atomic force microscope scans of HT-29 cells. Although inhibition of sEH in differentiated HT-29 and Caco2 cells led to an increase in sEH expression in both cell lines, this treatment had an opposite effect on CYP2J2 expression in HT-29 and Caco2 cells. In addition, tissue samples of colorectal carcinoma and adjacent normal tissues from 45 patients were immunostained for sEH and villin. We detected a significant decrease in the expression of both proteins in colorectal carcinoma in comparison to adjacent normal tissue, and the decrease in both sEH and villin expression revealed a moderate positive association. Taken together, our results showed that sEH is an important player in intestinal cell differentiation.

Modulation of μ-opioid receptor desensitization in peripheral sensory neurons by phosphoinositide 3-kinase γ

Neuroscience ◽  
2010 ◽  
Vol 169 (1) ◽  
pp. 449-454 ◽  
Author(s):  
C. König ◽  
O. Gavrilova-Ruch ◽  
G. Segond von Banchet ◽  
R. Bauer ◽  
M. Grün ◽  
...  
Keyword(s):  
Opioid Receptor ◽  
Sensory Neurons ◽  
Receptor Desensitization ◽  
Peripheral Sensory Neurons ◽  
Μ Opioid Receptor ◽  
Phosphoinositide 3 Kinase ◽  
Peripheral Sensory

scholarly journals A-kinase anchoring protein 79/150 coordinates metabotropic glutamate receptor sensitization of peripheral sensory neurons

Pain ◽  
2015 ◽  
Vol 156 (11) ◽  
pp. 2364-2372 ◽  
Author(s):  
Kalina Szteyn ◽  
Matthew P. Rowan ◽  
Ruben Gomez ◽  
Junhui Du ◽  
Susan M. Carlton ◽  
...  
Keyword(s):  
Glutamate Receptor ◽  
Sensory Neurons ◽  
Metabotropic Glutamate Receptor ◽  
Metabotropic Glutamate ◽  
Peripheral Sensory Neurons ◽  
Anchoring Protein ◽  
Peripheral Sensory

scholarly journals IN VITRO SOLUBLE EPOXIDE HYDROLASE ENZYME INHIBITORY ACTIVITY OF SOME NOVEL CHALCONE DERIVATIVES

2012 ◽  
Vol 2 (4) ◽  
Author(s):  
Kuppusamy Asokkumar ◽  
Lokeswari Prathyusha Tangella ◽  
Muthusamy Umamaheshwari ◽  
Thirumalaisamy Shivashanmugam ◽  
Varadharajan Subhadradevi ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Epoxide Hydrolase ◽  
Soluble Epoxide Hydrolase ◽  
Chalcone Derivatives
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