Atomic Force Microscopy (AFM) requires immobilization of samples to a suitable substrate. Immobilized samples must withstand forces, particularly lateral forces, exerted by the tip during imaging. Air-drying is the simplest way of immobilizing molecules onto a substrate. However, this often compromises the integrity of structural features because of forces at the air-liquid interface. In solution the most common method is simple adsorption. While very convenient, this method works only for a limited number of samples. To overcome this problem procedures involving covalent chemical cross-linking have been developed. We have developed an alternative AFM substrate based on the mussel adhesive protein (MAP) from Mytilus edulis. This substrate is simple to prepare and can immobilize a wide range of samples, including gold particles, silica spheres, proteins, and nucleic acids.MAP is a polyphenols marine adhesive by which the mussel attaches itself to different surfaces. Studies of purified MAP indicate that it possesses a unique chemistry.