Characterization of molecular assemblies on silicon surfaces by attenuated total reflectance infrared spectroscopy

2006 ◽  
Vol 499 (1-2) ◽  
pp. 8-12 ◽  
Author(s):  
Masato Ara ◽  
Ryo Yamada ◽  
Hirokazu Tada
Lab on a Chip ◽  
2021 ◽  
Author(s):  
David Perez-Guaita ◽  
Zack Richardson ◽  
G. Quintas ◽  
Julia Kuligowski ◽  
Diana Eva Bedolla ◽  
...  

Infrared Spectroscopy (IR) enables the direct and rapid characterization of cells at the molecular level. Achieving a rapid and consistent cell preparation is critical for the development of Point-of-Care diagnostics...


Materials ◽  
2021 ◽  
Vol 14 (4) ◽  
pp. 931
Author(s):  
Ioana-Codruţa Mirică ◽  
Gabriel Furtos ◽  
Ondine Lucaciu ◽  
Petru Pascuta ◽  
Mihaela Vlassa ◽  
...  

The aim of this research was to develop new electrospun membranes (EMs) based on polycaprolactone (PCL) with or without metronidazole (MET)/nano-hydroxyapatite (nHAP) content. New nHAP with a mean diameter of 34 nm in length was synthesized. X-ray diffraction (XRD) and attenuated total reflectance Fourier transform infrared spectroscopy (FTIR-ATR) were used for structural characterization of precursors and EMs. The highest mechanical properties (the force at maximum load, Young’s modulus and tensile strength) were found for the PCL membranes, and these properties decreased for the other samples in the following order: 95% PCL + 5% nHAP > 80% PCL + 20% MET > 75% PCL + 5% nHAP + 20% MET. The stiffness increased with the addition of 5 wt.% nHAP. The SEM images of EMs showed randomly oriented bead-free fibers that generated a porous structure with interconnected macropores. The fiber diameter showed values between 2 and 16 µm. The fiber diameter increased with the addition of nHAP filler and decreased when MET was added. New EMs with nHAP and MET could be promising materials for guided bone regeneration or tissue engineering.


2021 ◽  
Vol 13 (13) ◽  
pp. 1601-1611
Author(s):  
Way Koon Teoh ◽  
Noor Zuhartini Md Muslim ◽  
Md Lukmi Ismail ◽  
Kah Haw Chang ◽  
Ahmad Fahmi Lim Abdullah

Quick determination and discrimination of commercial hand sanitisers for forensic investigation.


RSC Advances ◽  
2016 ◽  
Vol 6 (94) ◽  
pp. 92065-92072 ◽  
Author(s):  
Sónia Silva ◽  
Flávia Tobaldini-Valerio ◽  
Sofia Costa-de-Oliveira ◽  
Mariana Henriques ◽  
Joana Azeredo ◽  
...  

Accurate Candida species identification remains a challenge due to their phenotypic and genotypic similarity.


Cartilage ◽  
2021 ◽  
pp. 194760352199322
Author(s):  
Vesa Virtanen ◽  
Ervin Nippolainen ◽  
Rubina Shaikh ◽  
Isaac O. Afara ◽  
Juha Töyräs ◽  
...  

Objective Joint injuries may lead to degeneration of cartilage tissue and initiate development of posttraumatic osteoarthritis. Arthroscopic surgeries can be used to treat joint injuries, but arthroscopic evaluation of articular cartilage quality is subjective. Fourier transform infrared spectroscopy combined with fiber optics and attenuated total reflectance crystal could be used for the assessment of tissue quality during arthroscopy. We hypothesize that fiber-optic mid-infrared spectroscopy can detect enzymatically and mechanically induced damage similar to changes occurring during progression of osteoarthritis. Design Bovine patellar cartilage plugs were extracted and degraded enzymatically and mechanically. Adjacent untreated samples were utilized as controls. Enzymatic degradation was done using collagenase and trypsin enzymes. Mechanical damage was induced by (1) dropping a weight impactor on the cartilage plugs and (2) abrading the cartilage surface with a rotating sandpaper. Fiber-optic mid-infrared spectroscopic measurements were conducted before and after treatments, and spectral changes were assessed with random forest, partial least squares discriminant analysis, and support vector machine classifiers. Results All models had excellent classification performance for detecting the different enzymatic and mechanical damage on cartilage matrix. Random forest models achieved accuracies between 90.3% and 77.8%, while partial least squares model accuracies ranged from 95.8% to 84.7%, and support vector machine accuracies from 91.7% to 80.6%. Conclusions The results suggest that fiber-optic Fourier transform infrared spectroscopy attenuated total reflectance spectroscopy is a viable way to detect minor and major degeneration of articular cartilage. Objective measures provided by fiber-optic spectroscopic methods could improve arthroscopic evaluation of cartilage damage.


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