Effect of oxidative stress and endotoxin on human serum albumin in brain-dead organ donors

2012 ◽  
Vol 159 (6) ◽  
pp. 487-496 ◽  
Author(s):  
Bettina Leber ◽  
Vanessa Stadlbauer ◽  
Philipp Stiegler ◽  
Stefanie Stanzer ◽  
Ursula Mayrhauser ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Organ Donors ◽  
Brain Dead

scholarly journals Allysine and α-Aminoadipic Acid as Markers of the Glyco-Oxidative Damage to Human Serum Albumin under Pathological Glucose Concentrations

Antioxidants ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 474 ◽  
Author(s):  
Carolina Luna ◽  
Alexis Arjona ◽  
Carmen Dueñas ◽  
Mario Estevez
Keyword(s):  
Oxidative Stress ◽  
Plasma Concentration ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Typical Feature ◽  
Advanced Glycation ◽  
Chemical Mechanisms ◽  
Fasting Plasma ◽  
Insight Into

Understanding the molecular basis of the disease is of the utmost scientific interest as it contributes to the development of targeted strategies of prevention, diagnosis, and therapy. Protein carbonylation is a typical feature of glyco-oxidative stress and takes place in health disorders such as diabetes. Allysine as well as its oxidation product, the α-amino adipic acid (α-AA) have been found to be markers of diabetes risk whereas little is known about the chemistry involved in its formation under hyperglycemic conditions. To provide insight into this issue, human serum albumin was incubated in the presence of FeCl3 (25 μM) and increasing glucose concentrations for 32 h at 37 °C. These concentrations were selected to simulate (i) physiological fasting plasma concentration (4 mM), (ii) pathological pre-diabetes fasting plasma concentration (8 mM), and pathological diabetes fasting plasma concentration (12 mM) of glucose. While both allysine and α-AA were found to increase with increasing glucose concentrations, the carboxylic acid was only detected at pathological glucose concentrations and appeared to be a more reliable indicator of glyco-oxidative stress. The underlying chemical mechanisms of lysine glycation as well as of the depletion of tryptophan and formation of fluorescent and colored advanced glycation products are discussed.

scholarly journals Cys34-Cysteinylated Human Serum Albumin Is a Sensitive Plasma Marker in Oxidative Stress-Related Chronic Diseases

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e85216 ◽  
Author(s):  
Kohei Nagumo ◽  
Motohiko Tanaka ◽  
Victor Tuan Giam Chuang ◽  
Hiroko Setoyama ◽  
Hiroshi Watanabe ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Chronic Diseases ◽  
Plasma Marker

scholarly journals Oxidation of Human Serum Albumin Exhibits Inter-Individual Variability after an Ultra-Marathon Mountain Race

2017 ◽  
Author(s):  
Ypatios Spanidis ◽  
Alexandros Priftis ◽  
Dimitrios Stagos ◽  
George A. Stravodimos ◽  
Demetres D. Leonidas ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Reduction Potential ◽  
Individual Variability ◽  
Protein Carbonyls ◽  
Marathon Race ◽  
Oxidation Reduction ◽  
Oxidation Reduction Potential

The aim of this study was to examine the oxidation of human serum albumin (HSA) caused by oxidative stress after an exhaustive exercise such as ultra-marathon race. Thus, blood samples from 12 adult runners who underwent a 103 km mountain ultra-marathon race were collected pre- and 24, 48 and 72 h post race. HSA was partially purified using affinity chromatography and then was subjected to Western blot analysis for disulfide dimers determination, indicating oxidation. The results were correlated with those from a previous study in which the same samples were analyzed using different oxidative stress markers and a good correlation with protein carbonyls (PC) at all time points was observed. Moreover there was a significant correlation with static oxidation-reduction potential (sORP) at 24 h, and a negative correlation with capacity oxidation-reduction potential (cORP) at 24 and 48 h. In addition, an individual analysis of albumin dimers exhibited great inter-individual differences. This inter-individual variability in the oxidation of HSA may suggest different interventions (e.g. through diet) in order to confront the effects on athletes’ organism after a strenuous exercise. In conclusion, this study supported the importance of the assessment of albumin dimers as a predictive marker for exercise-induced oxidative stress.

Human Serum Albumin and Its Relation with Oxidative Stress

2013 ◽  
Vol 59 (09+10/2013) ◽  
Author(s):  
Mustafa Sitar ◽  
Seval Aydin ◽  
Ufuk çAkatay
Keyword(s):  
Oxidative Stress ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum

pH-responsive Pt-based nanoradiosensitizer for enhanced radiotherapy via oxidative stress amplification

Nanoscale ◽  
2021 ◽  
Author(s):  
Licheng Yu ◽  
Xiaolei Zhang ◽  
Xiaomin Li ◽  
Zhenjie Zhang ◽  
Xiaoyan Niu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Chloroplatinic Acid ◽  
Ph Responsive ◽  
Simple Method ◽  
Stress Amplification

Tumor radioresistance is a major issue in radiotherapy. To address it, a pH-responsive nanoradiosensitizer was synthesized employing a simple method. Initially, chloroplatinic acid was reduced by human serum albumin (HSA)...

scholarly journals Neuroprotection Mediated by Human Blood Plasma in Mouse Hippocampal Slice Cultures and in Oxidatively Stressed Human Neurons

2021 ◽  
Vol 22 (17) ◽  
pp. 9567
Author(s):  
Lucia M. Ruiz-Perera ◽  
Anna L. Höving ◽  
Kazuko E. Schmidt ◽  
Sule Cenan ◽  
Max Wohllebe ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Neurodegenerative Diseases ◽  
Blood Plasma ◽  
Human Serum ◽  
Human Plasma ◽  
Neuroprotective Effect ◽  
Human Blood Plasma ◽  
Human Neurons

Neuroprotection from oxidative stress is critical during neuronal development and maintenance but also plays a major role in the pathogenesis and potential treatment of various neurological disorders and neurodegenerative diseases. Emerging evidence in the murine system suggests neuroprotective effects of blood plasma on the aged or diseased brain. However, little is known about plasma-mediated effects on human neurons. In the present study, we demonstrate the neuroprotective effect mediated by human plasma and the most abundant plasma–protein human serum albumin against oxidative stress in glutamatergic neurons differentiated from human neural crest-derived inferior turbinate stem cells. We observed a strong neuroprotective effect of human plasma and human serum albumin against oxidative stress-induced neuronal death on the single cell level, similar to the one mediated by tumor necrosis factor alpha. Moreover, we detected neuroprotection of plasma and human serum albumin against kainic acid-induced excitatory stress in ex vivo cultured mouse hippocampal tissue slices. The present study provides deeper insights into plasma-mediated neuroprotection ultimately resulting in the development of novel therapies for a variety of neurological and, in particular, neurodegenerative diseases.

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