Inhibition of Swine Leukocyte Antigen-I Presentation in Transgenic Mini-pig Cell Lines by Expressing Human Cytomegalovirus US6

2010 ◽  
Vol 42 (10) ◽  
pp. 4648-4650 ◽  
Author(s):  
J.Y. Yoo ◽  
K.M. Choi ◽  
S.P. Hong ◽  
S.H. Kim ◽  
K.-W. Park ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cytomegalovirus ◽  
Swine Leukocyte Antigen ◽  
Leukocyte Antigen

193 HUMAN CYTOMEGALOVIRUS PROTEIN US11 DOWN-REGULATES THE EXPRESSION OF SWINE LEUKOCYTE ANTIGEN-I IN MINIPIG CELLS

2008 ◽  
Vol 20 (1) ◽  
pp. 176
Author(s):  
J. Y. Yoo ◽  
K. M. Choi ◽  
S. P. Hong ◽  
G. S. Han ◽  
E. J. Kim ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cytomegalovirus ◽  
Cytotoxic T Lymphocyte ◽  
Early Stage ◽  
Swine Leukocyte Antigen ◽  
Leukocyte Antigen ◽  
Clonal Cell ◽  
Heavy Chains ◽  
Fetal Fibroblasts ◽  
Clonal Cell Lines

The CD8+ cytotoxic T lymphocyte (CTL)-mediated immune response is important in porcine xenotransplantation, and pigs could be used as a good model for organ donor if these immunological barriers are overcome. Human cytomegalovirus (HCMV) encodes unique short (US) 11 gene, which interferes with cellular immune responses by inducing rapid degradation of newly synthesized heavy chains of major histocompatibility (MHC) class I. The destruction of heavy chains by US11 helps the virus to hide from recognition by cytotoxic T lymphocytes at early stage. In this study we demonstrated the inhibitory effect of US11 on the cytotoxicity of CTL cells by down-regulation of swine leukocyte antigen (SLA)-I expression. We established five US11 clonal cell lines by transfection into minipig fetal fibroblasts and confirmed the integration of US11 gene by PCR and Southern blot assay. The reduction of SLA-I, which was expressed on the cell surface, was also detected by flow cytometry assay. The level (14.6% to 21.2%) of SLA-1 expression in US11 clonal cell lines was decreased relative to that in the control. In the CTL assay, the rate of CD8+ T cell-mediated cytotoxicity was significantly reduced to 31.9 � 11.3%, compared to that of the control (81.4 � 5.3%; P < 0.01; n = 4). These results indicate that HCMV viral protein US11 can effectively suppress the presentation of SLA-I in pig fetal fibroblast cells. This work was supported by a grant (Code # 20070101034005) from the BioGreen 21 Program, Rural Development Administration, Republic of Korea.

A NONCLASSICAL CLASS I SWINE LEUKOCYTE ANTIGEN (PD-6) FUNCTION AS A NOVEL PORCINE NATURAL KILLER CELL INHIBITORY MOLECULE.

2000 ◽  
Vol 69 (Supplement) ◽  
pp. S384
Author(s):  
Hitomi Sasaki ◽  
Xiao-Chun Xu ◽  
Bashoo Naziruddin ◽  
Toru Higuchi ◽  
Douglas M. Smith ◽  
...  
Keyword(s):  
Natural Killer Cell ◽  
Natural Killer ◽  
Killer Cell ◽  
Class I ◽  
Swine Leukocyte Antigen ◽  
Leukocyte Antigen ◽  
Inhibitory Molecule ◽  
Nonclassical Class

scholarly journals Effect of Genetic Diversity in Swine Leukocyte Antigen-DRA Gene on Piglet Diarrhea

Genes ◽  
2016 ◽  
Vol 7 (7) ◽  
pp. 36 ◽  
Author(s):  
Xiaoyu Huang ◽  
Qiaoli Yang ◽  
Junhu Yuan ◽  
Lixia Liu ◽  
Wenyang Sun ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Swine Leukocyte Antigen ◽  
Leukocyte Antigen

scholarly journals Swine leukocyte antigen class II genes (SLA-DRA, SLA-DRB1, SLA-DQA, SLA-DQB1) polymorphism and genotyping in Guizhou minipigs

2015 ◽  
Vol 14 (4) ◽  
pp. 15256-15266 ◽  
Author(s):  
Z.Z. Liu ◽  
J.H. Xia ◽  
L.L. Xin ◽  
Z.G. Wang ◽  
L. Qian ◽  
...  
Keyword(s):  
Class Ii ◽  
Swine Leukocyte Antigen ◽  
Leukocyte Antigen

scholarly journals Development of Cell Lines That Provide Tightly Controlled Temporal Translation of the Human Cytomegalovirus IE2 Proteins for Complementation and Functional Analyses of Growth-Impaired and Nonviable IE2 Mutant Viruses

2008 ◽  
Vol 82 (14) ◽  
pp. 7059-7077 ◽  
Author(s):  
Rebecca L. Sanders ◽  
Charles L. Clark ◽  
Christopher S. Morello ◽  
Deborah H. Spector
Keyword(s):  
Cell Lines ◽  
Human Cytomegalovirus ◽  
Dna Recombination ◽  
Virus Production ◽  
Protein Translation ◽  
Temporal Expression ◽  
Late Gene Expression ◽  
Artificial Chromosomes ◽  
Functional Analyses ◽  
Early Late

ABSTRACT The human cytomegalovirus (HCMV) IE2 86 protein is essential for viral replication. Two other proteins, IE2 60 and IE2 40, which arise from the C-terminal half of IE2 86, are important for later stages of the infection. Functional analyses of IE2 86 in the context of the infection have utilized bacterial artificial chromosomes as vectors to generate mutant viruses. One limitation is that many mutations result in debilitated or nonviable viruses. Here, we describe a novel system that allows tightly controlled temporal expression of the IE2 proteins and provides complementation of both growth-impaired and nonviable IE2 mutant viruses. The strategy involves creation of cell lines with separate lentiviruses expressing a bicistronic RNA with a selectable marker as the first open reading frame (ORF) and IE2 86, IE2 60, or IE2 40 as the second ORF. Induction of expression of the IE2 proteins occurs only following DNA recombination events mediated by Cre and FLP recombinases that delete the first ORF. HCMV encodes Cre and FLP, which are expressed at immediate-early (for IE2 86) and early-late (for IE2 40 and IE2 60) times, respectively. We show that the presence of full-length IE2 86 alone provides some complementation for virus production, but the correct temporal expression of IE2 86 and IE2 40 together has the most beneficial effect for early-late gene expression and synthesis of infectious virus. This approach for inducible protein translation can be used for complementation of other mutations as well as controlled expression of toxic cellular and microbial proteins.

The infection of human primary cells and cell lines by human cytomegalovirus: New tropism and new reservoirs for HCMV

2008 ◽  
Vol 131 (2) ◽  
pp. 160-169 ◽  
Author(s):  
Wei Wang ◽  
Ping Yu ◽  
Peng Zhang ◽  
Yujun Shi ◽  
Hong Bu ◽  
...  
Keyword(s):  
Cell Lines ◽  
Human Cytomegalovirus ◽  
Primary Cells ◽  
Human Primary Cells

Characterization of swine leukocyte antigen polymorphism by sequence-based and PCR-SSP methods in Meishan pigs

2006 ◽  
Vol 58 (11) ◽  
pp. 873-882 ◽  
Author(s):  
Chak-Sum Ho ◽  
Erin S. Rochelle ◽  
Gregory W. Martens ◽  
Lawrence B. Schook ◽  
Douglas M. Smith
Keyword(s):  
Swine Leukocyte Antigen ◽  
Leukocyte Antigen
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