scholarly journals Variants of glutathione s-transferase pi 1 exhibit differential enzymatic activity and inhibition by heavy metals

2012 ◽  
Vol 26 (4) ◽  
pp. 630-635 ◽  
Author(s):  
Jaclyn M. Goodrich ◽  
Niladri Basu
Keyword(s):  
Heavy Metals ◽  
Enzymatic Activity ◽  
Glutathione S Transferase

scholarly journals Oxidative stress and antioxidant indices of the marine red alga Porphyra vietnamensis

2013 ◽  
Vol 72 (2) ◽  
pp. 197-209 ◽  
Author(s):  
Navnath M. Pise ◽  
Dattatry K. Gaikwad ◽  
Tanaji G. Jagtap
Keyword(s):  
Oxidative Stress ◽  
Heavy Metals ◽  
Red Alga ◽  
Glutathione S Transferase ◽  
Antioxidant Defence ◽  
Antioxidant Defences ◽  
Pollution Levels ◽  
Stress Markers ◽  
Marine Habitats ◽  
Assessment And Monitoring

Abstract -Oxidative stress and antioxidant defence systems were assessed in a marine red alga Porphyra vietnamensis Tanaka et Pham-Hoang Ho, from India. Lipid peroxidation (LPX) and hydrogen peroxide (H2O2) were measured as oxidative stress markers. Antioxidant defences were measured as catalase (CAT), glutathione S-transferase (GST) and ascorbic acid (AsA), in order to understand their dissimilarity with respect to environmental conditions (pollution levels) from selective locations along the central west coast of India. Levels of LPX, H2O2, CAT and GST were significantly higher in samples collected from Dona Paula than in samples from Malvan and Kunkeshwar, while a lower concentration of AsA was found in samples from Dona Paula. Heavy metals such as Cd, Pb and Hg in higher concentrations in these areas than in other sites were also observed. Variation of oxidative stress indices in response to the accumulation of heavy metals within P. vietnamensis could be used as molecular biomarkers for the assessment and monitoring of environmental quality in ecologically sensitive marine habitats.

Enzymatic Activity of Glutathione S-Transferase and Dental Fluorosis Among Children Receiving Two Different Levels of Naturally Fluoridated Water

2016 ◽  
Vol 176 (1) ◽  
pp. 40-47 ◽  
Author(s):  
Irvin Bonola-Gallardo ◽  
María Esther Irigoyen-Camacho ◽  
Liliana Vera-Robles ◽  
Antonio Campero ◽  
Luis Gómez-Quiroz
Keyword(s):  
Enzymatic Activity ◽  
Dental Fluorosis ◽  
Glutathione S Transferase ◽  
Different Levels

scholarly journals Diagnosis of the Content of Selected Heavy Metals in the Soils of the Pałuki Region Against their Enzymatic Activity

2013 ◽  
Vol 39 (3) ◽  
pp. 23-32 ◽  
Author(s):  
Joanna Lemanowicz ◽  
Agata Bartkowiak
Keyword(s):  
Heavy Metals ◽  
Enzymatic Activity ◽  
Plant Species ◽  
Hydrolytic Enzymes ◽  
Soil Samples ◽  
River Valley ◽  
Vegetable Crops ◽  
Soil Material ◽  
The Right ◽  
Very High

Abstract The paper presents the research results for the soils sampled from the area located in the eastern part of the Chodzieskie Lakes, between the Middle Noteć River Valley and the Wełna River Valley, the right tributary of the Warta River. The research involved 7 soil samples from the surface horizons, allocated to the cultivation of various plant species (cereals and vegetable crops). The following were determined in the soil material: the content of phytoavailable forms of selected heavy metals Zn, Cu, Pb, Ni, Fe and Mn, active and available to plants phosphorus against the activity of selected oxydo-reduction and hydrolytic enzymes. The soil under the vegetable crops showed a very high richness in phosphorus available to plants, which must have been related to an intensive fertilisation. There were identified relatively low contents of the available forms of the heavy metals investigated, the fact that points to their natural content in soil, which triggered the inhibition of neither the oxydo-reduction nor hydrolytic enzymes.

scholarly journals Biochar alleviates metal toxicity and improves microbial community functions in a soil co-contaminated with cadmium and lead

Biochar ◽  
2021 ◽  
Author(s):  
Nahid Azadi ◽  
Fayez Raiesi
Keyword(s):  
Heavy Metals ◽  
Low Temperature ◽  
Organic Carbon ◽  
Enzymatic Activity ◽  
Microbial Activity ◽  
Contaminated Soils ◽  
Metal Availability ◽  
Soil Microbial ◽  
Labile C ◽  
Cadmium And Lead

AbstractSoil amendment with biochar alleviates the toxic effects of heavy metals on microbial functions in single-metal contaminated soils. Yet, it is unclear how biochar application would improve microbial activity and enzymatic activity in soils co-polluted with toxic metals. The present research aimed at determining the response of microbial and biochemical attributes to addition of sugarcane bagasse biochar (SCB) in cadmium (Cd)-lead (Pb) co-contaminated soils. SCBs (400 and 600 °C) decreased the available concentrations of Cd and Pb, increased organic carbon (OC) and dissolved organic carbon (DOC) contents in soil. The decrease of metal availability was greater with 600 °C SCB than with 400 °C SCB, and metal immobilization was greater for Cd (16%) than for Pb (12%) in co-spiked soils amended with low-temperature SCB. Biochar application improved microbial activity and biomass, and enzymatic activity in the soils co-spiked with metals, but these positive impacts of SCB were less pronounced in the co-spiked soils than in the single-spiked soils. SCB decreased the adverse impacts of heavy metals on soil properties largely through the enhanced labile C for microbial assimilation and partly through the immobilization of metals. Redundancy analysis further confirmed that soil OC was overwhelmingly the dominant driver of changes in the properties and quality of contaminated soils amended with SCB. The promotion of soil microbial quality by the low-temperature SCB was greater than by high-temperature SCB, due to its higher labile C fraction. Our findings showed that SCB at lower temperatures could be applied to metal co-polluted soils to mitigate the combined effects of metal stresses on microbial and biochemical functions.

scholarly journals Relationship of Impairment of Schistosome 28-Kilodalton Glutathione S-Transferase (GST) Activity to Expression of Immunity to Schistosoma mattheei in Calves Vaccinated with Recombinant Schistosoma bovis28-Kilodalton GST

1998 ◽  
Vol 66 (3) ◽  
pp. 1142-1148 ◽  
Author(s):  
Jean-Marie Grzych ◽  
Jan De Bont ◽  
Jinli Liu ◽  
Jean-Loup Neyrinck ◽  
Josette Fontaine ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Natural Infection ◽  
Egg Load ◽  
Glutathione S Transferase ◽  
Catalytic Function ◽  
Schistosoma Bovis ◽  
Specific Immunoglobulin ◽  
Iga Antibody ◽  
Relationship Of ◽  
Gst Activity

ABSTRACT Sera from calves vaccinated with the recombinant Schistosoma bovis-derived 28-kDa glutathione S-transferase (28GST) and subsequently naturally or experimentally exposed toSchistosoma mattheei were studied for their content of specific immunoglobulin G (IgG) and IgA antibodies to recombinantS. bovis 28GST as well as for their capacity to inhibit the enzymatic activity of the antigen. The results were analyzed in regard to the presence (natural infection) or absence (experimental infection) of a protective effect(s) (reductions in worm burden, egg load, fecal egg counts, and excretion of viable eggs) toward S. mattheei challenge. Under such conditions, no differences in the IgG- and IgA-specific antibodies to recombinant S. bovis28GST or in the ability to block the catalytic function of the antigen between the two groups were recorded. Nevertheless, correlation analysis between the specific antibody responses to recombinantS. bovis 28GST and the inhibition of GST activity suggested an association with IgG in experimentally infected vaccinated animals, while in naturally infected vaccinated calves, the inhibitory activity appeared to be linked to a greater degree with IgA. These results suggest that in contrast to schistosomiasis in humans, IgG antibodies in calves with schistosomiasis may exhibit inhibitory functions toward GST enzymatic activity or have a modulatory effect on IgA antibody properties. Furthermore, sera from animals immunized with recombinantS. bovis 28GST recognized the native S. mattheei 28GST and achieved comparable levels of inhibition of activity of recombinant S. bovis 28GST and S. matthei 28GST, indicating the presence of cross-reactive epitopes on these two molecules.

Impact of heavy metals on enzymatic activity of substrate and on composting worms Eisenia fetida

2006 ◽  
Vol 97 (13) ◽  
pp. 1498-1502 ◽  
Author(s):  
Christopher Malley ◽  
Jaya Nair ◽  
Goen Ho
Keyword(s):  
Heavy Metals ◽  
Enzymatic Activity ◽  
Eisenia Fetida

scholarly journals Heavy Metal-Induced Changes in the Glutathione Levels and Glutathione Reductase/Glutathione S-Transferase Activities in the Liver of Male Mice

1998 ◽  
Vol 17 (4) ◽  
pp. 383-392 ◽  
Author(s):  
Salah A. Sheweita
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Single Dose ◽  
Glutathione Reductase ◽  
Lead Acetate ◽  
Cadmium Chloride ◽  
Cobalt Chloride ◽  
Male Mice ◽  
Glutathione S Transferase ◽  
Hepatic Level

Glutathione S-transferases catalyze the metabolism of reactive substances of exogenous or endogenous origin and are involved in inactivation processes of xenobiotics and their metabolites. The present study aims at investigating the influence of heavy metals on the hepatic level of reduced glutathione (GSH), glutathione S-transferase, and glutathione reductase activities in the liver of male mice after single-dose (1 and 24 h) and repeated-dose treatments (three consecutive days). The hepatic level of GSH was depleted after single-dose treatments with cadmium chloride, mercuric chloride, cobalt chloride, cesium chloride, lead acetate, and silver nitrate, and percentage of GSH depletion was greater still after the repeated-dose treatments. Nickel chloride, on the other hand, did not cause any change in the level of GS H after any period of treatment. Glutathione reductase activity was increased 24 hours after treatment with cadmium chloride, mercuric chloride, lead acetate, and silver nitrate, whereas cobalt chloride decreased such activity after repeated doses. With the exception of cadmium chloride, glutathione S-transferase activity was significantly decreased 24 hours after a single-dose treatment with all of the tested heavy metals. Such alterations in the activities of phase II drug-metabolizing enzymes as a result of heavy metal treatment may change the hepatic capacity for the detoxification of many toxic compounds from endogenous or exogenous sources.

Circular dichroic evidence for regulation of enzymatic activity by nonsubstrate hydrophobic ligand on glutathione S-transferase P

1992 ◽  
Vol 189 (2) ◽  
pp. 1243-1251 ◽  
Author(s):  
Jun Nishihira ◽  
Teruo Ishibashi ◽  
Masaharu Sakai ◽  
Shinzo Nishi ◽  
Takashi Kumazaki ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Glutathione S Transferase ◽  
Hydrophobic Ligand ◽  
Circular Dichroic

Effect of heavy metals on microbial communities and enzymatic activity in soil column experiment

2006 ◽  
Vol 52 (2) ◽  
pp. 161-169 ◽  
Author(s):  
R. Vaisvalavicius ◽  
A. Motuzas ◽  
I. Prosycevas ◽  
L. Levinskaite ◽  
D. Zakarauskaite ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Enzymatic Activity ◽  
Microbial Communities ◽  
Soil Column ◽  
Column Experiment ◽  
Soil Column Experiment
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