Characterization of human liver cytochromes P450 by combining the biochemical and proteomic approaches

2006 ◽  
Vol 20 (6) ◽  
pp. 966-974 ◽  
Author(s):  
N.A. Petushkova ◽  
I.P. Kanaeva ◽  
A.V. Lisitsa ◽  
G.F. Sheremetyeva ◽  
V.G. Zgoda ◽  
...  
Keyword(s):  
Human Liver ◽  
Cytochromes P450

scholarly journals Characterization of O-demethylations and Aromatic Hydroxylations Mediated by Cytochromes P450 in the Metabolism of Flavonoid Aglycons

2019 ◽  
Vol 92 (1) ◽  
pp. 115-123 ◽  
Author(s):  
Goran Benković ◽  
Hrvoje Rimac ◽  
Željan Maleš ◽  
Siniša Tomić ◽  
Zoran Lončar ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Human Liver ◽  
Cytochromes P450 ◽  
Liver Microsomes ◽  
Cytochrome P450 Enzymes ◽  
Uv Detector ◽  
Aromatic Hydroxylation ◽  
Metabolism Of Xenobiotics ◽  
Kinetics Of

One of the most important groups of metabolic enzymes is cytochrome P450 superfamily. These enzymes are important in terms of the catalytic diversity and the large number of xenobiotics that are detoxified or activated by converting to reactive metabolites. Flavonoids are xenobiotics to which humans are exposed through diet. Data on their oxidative metabolism mediated by cytochromes P450 are limited. The aim of this study was to determine the enzymatic kinetics of O-demethylation and aromatic hydroxylation of flavonoid aglycons on recombinant cytochrome P450 enzymes and human liver microsomes systems. The study was performed on ten flavonoids, namely 3,7-dihydroxyflavone, 7-hydroxyflavone, acacetin, apigenin, flavone, galangin, kaempferol, naringenin, sakuranetin, and tangeretin using liquid chromatography coupled with mass spectrometry and UV detector. Most relevant enzyme involved in metabolism of flavonoid aglycons is CYP1A2, and its catalytic effectiveness ranges from 0.5 to 2.9 × 106 M–1 min–1. Having in mind high expression and involvement of CYP1A2 in metabolism of xenobiotics including drugs, and its intraindividual differences in expression and activity, potential of drug-flavonoid competitive interactions/inhibitions should be considered when consuming dietary supplement and foods rich in flavonoids.

CHARACTERIZATION OF TESTOSTERONE 11β-HYDROXYLATION CATALYZED BY HUMAN LIVER MICROSOMAL CYTOCHROMES P450

2005 ◽  
Vol 33 (6) ◽  
pp. 714-718 ◽  
Author(s):  
Man Ho Choi ◽  
Paul L. Skipper ◽  
John S. Wishnok ◽  
Steven R. Tannenbaum
Keyword(s):  
Human Liver ◽  
Cytochromes P450

Characterization of brimonidine metabolism with rat, rabbit, dog, monkey and human liver fractions and rabbit liver aldehyde oxidase

Xenobiotica ◽  
1996 ◽  
Vol 26 (1) ◽  
pp. 1035-1055 ◽  
Author(s):  
A. A. Acheampong ◽  
D-S. Chien ◽  
S. Lam ◽  
S. Vekich ◽  
A. Breau ◽  
...  
Keyword(s):  
Human Liver ◽  
Aldehyde Oxidase ◽  
Rabbit Liver

scholarly journals Isolation and characterization of the apolipoprotein E receptor from canine and human liver.

1986 ◽  
Vol 261 (9) ◽  
pp. 4256-4267 ◽  
Author(s):  
D Y Hui ◽  
W J Brecht ◽  
E A Hall ◽  
G Friedman ◽  
T L Innerarity ◽  
...  
Keyword(s):  
Apolipoprotein E ◽  
Human Liver ◽  
Isolation And Characterization

scholarly journals Purification and characterization of cytochrome P-450-dependent arachidonic acid epoxygenase from human liver.

1988 ◽  
Vol 263 (5) ◽  
pp. 2536-2542
Author(s):  
M Laniado-Schwartzman ◽  
K L Davis ◽  
J C McGiff ◽  
R D Levere ◽  
N G Abraham
Keyword(s):  
Arachidonic Acid ◽  
Human Liver ◽  
Purification And Characterization ◽  
Cytochrome P 450

scholarly journals Characterization of the antioxidant enzyme, thioredoxin peroxidase, from the carcinogenic human liver fluke, Opisthorchis viverrini

2008 ◽  
Vol 160 (2) ◽  
pp. 116-122 ◽  
Author(s):  
Sutas Suttiprapa ◽  
Alex Loukas ◽  
Thewarach Laha ◽  
Sopit Wongkham ◽  
Sasithorn Kaewkes ◽  
...  
Keyword(s):  
Antioxidant Enzyme ◽  
Human Liver ◽  
Liver Fluke ◽  
Opisthorchis Viverrini ◽  
Thioredoxin Peroxidase

Architectural and Immunohistochemical Characterization of Biliary Ductules in Normal Human Liver

2009 ◽  
Vol 18 (10) ◽  
pp. 1417-1422 ◽  
Author(s):  
Katalin Dezső ◽  
Sándor Paku ◽  
Veronika Papp ◽  
Eszter Turányi ◽  
Peter Nagy
Keyword(s):  
Human Liver ◽  
Normal Human Liver ◽  
Normal Human
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