Crossing the Intestinal Barrier via Listeria Adhesion Protein and Internalin A

2019 ◽  
Vol 27 (5) ◽  
pp. 408-425 ◽  
Author(s):  
Rishi Drolia ◽  
Arun K. Bhunia
Keyword(s):  
Intestinal Barrier ◽  
Adhesion Protein ◽  
Listeria Adhesion Protein ◽  
Internalin A

scholarly journals Listeria adhesion protein-expressing bioengineered probiotics prevent fetoplacental transmission of Listeria monocytogenes in a pregnant Guinea pig model

2021 ◽  
Vol 151 ◽  
pp. 104752
Author(s):  
Valerie E. Ryan ◽  
Taylor W. Bailey ◽  
Dongqi Liu ◽  
Tracy Vemulapalli ◽  
Bruce Cooper ◽  
...  
Keyword(s):  
Listeria Monocytogenes ◽  
Guinea Pig ◽  
Pig Model ◽  
Adhesion Protein ◽  
Listeria Adhesion Protein ◽  
Guinea Pig Model

scholarly journals Recombinant Probiotic Expressing Listeria Adhesion Protein Attenuates Listeria monocytogenes Virulence In Vitro

PLoS ONE ◽  
2012 ◽  
Vol 7 (1) ◽  
pp. e29277 ◽  
Author(s):  
Ok Kyung Koo ◽  
Mary Anne Roshni Amalaradjou ◽  
Arun K. Bhunia
Keyword(s):  
Listeria Monocytogenes ◽  
Adhesion Protein ◽  
Listeria Adhesion Protein

scholarly journals Variation inListeria monocytogenesDose Responses in Relation to Subtypes Encoding a Full-Length or Truncated Internalin A

2010 ◽  
Vol 77 (4) ◽  
pp. 1171-1180 ◽  
Author(s):  
Yuhuan Chen ◽  
William H. Ross ◽  
Richard C. Whiting ◽  
Anna Van Stelten ◽  
Kendra K. Nightingale ◽  
...  
Keyword(s):  
Stop Codon ◽  
Premature Stop Codon ◽  
Intestinal Barrier ◽  
Full Length ◽  
Quantitative Evidence ◽  
Consumption Data ◽  
Genes Encoding ◽  
A Cell ◽  
Dose Responses ◽  
Internalin A

ABSTRACTInternalin A (InlA; encoded byinlA) facilitates the crossing of the intestinal barrier byListeria monocytogenes. Mutations leading to a premature stop codon (PMSC) ininlAand thus attenuated mammalian virulence have been reported. We recently characterized 502L. monocytogenesfood isolates from a retail survey and 507 human clinical isolates from multiple U.S. states with respect to the presence/absence ofinlAmutations. The objective of this study was to investigate the hypothesis that dose responses for human listeriosis vary betweenL. monocytogenesstrains with and those without a PMSC ininlA. Subtype-specific prevalence and concentration distributions in food, along with epidemiologic and consumption data, were input into established dose-response models to generate anrvalue (probability of a cell causing illness). Under the conservative assumption thatL. monocytogeneslevels at retail represent levels consumed, mean log10rvalues were −8.1 and −10.7 forL. monocytogenessubtypes with genes encoding a full-length and a truncated InlA, respectively.L. monocytogenescarrying a 5′ frameshift mutation in a homopolymeric tract showed a mean log10rvalue of −12.1. Confidence intervals for thervalues and their differences varied depending on subtypes. When the increase in concentration ofL. monocytogenessubtypes between retail and consumption was considered, mean log10rvalues were reduced to −10.4, −13.8, and −12.8 for the subtypes with genes encoding a full-length InlA, for the subtypes carrying a PMSC ininlA, and for allL. monocytogenesisolates regardless of subtype, respectively. Our study provides further quantitative evidence thatL. monocytogenessubtypes vary in abilities and relative likelihoods of causing human disease, which were mechanistically related to defined genetic markers.

scholarly journals Receptor-targeted engineered probiotics mitigate lethal Listeria infection

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Rishi Drolia ◽  
Mary Anne Roshni Amalaradjou ◽  
Valerie Ryan ◽  
Shivendra Tenguria ◽  
Dongqi Liu ◽  
...  
Keyword(s):  
Intestinal Barrier ◽  
Probiotic Strain ◽  
Nuclear Factor Κb ◽  
Heat Shock Protein 60 ◽  
New Paradigm ◽  
Barrier Dysfunction ◽  
Adhesion Protein ◽  
Systemic Dissemination ◽  
Lethal Infection ◽  
Listeria Infection

AbstractProbiotic bacteria reduce the intestinal colonization of pathogens. Yet, their use in preventing fatal infection caused by foodborne Listeria monocytogenes (Lm), is inconsistent. Here, we bioengineered Lactobacillusprobiotics (BLP) to express the Listeria adhesion protein (LAP) from a non-pathogenic Listeria (L. innocua) and a pathogenic Listeria (Lm) on the surface of Lactobacillus casei. The BLP strains colonize the intestine, reduce Lm mucosal colonization and systemic dissemination, and protect mice from lethal infection. The BLP competitively excludes Lm by occupying the surface presented LAP receptor, heat shock protein 60 and ameliorates the Lm-induced intestinal barrier dysfunction by blocking the nuclear factor-κB and myosin light chain kinase-mediated redistribution of the major epithelial junctional proteins. Additionally, the BLP increases intestinal immunomodulatory functions by recruiting FOXP3+T cells, CD11c+ dendritic cells and natural killer cells. Engineering a probiotic strain with an adhesion protein from a non-pathogenic bacterium provides a new paradigm to exclude pathogens and amplify their inherent health benefits.

scholarly journals Listeria monocytogenes Relies on the Heme-Regulated Transporter hrtAB to Resist Heme Toxicity and Uses Heme as a Signal to Induce Transcription of lmo1634, Encoding Listeria Adhesion Protein

2018 ◽  
Vol 9 ◽  
Author(s):  
Patrícia Teixeira dos Santos ◽  
Pernille Tholund Larsen ◽  
Pilar Menendez-Gil ◽  
Eva Maria Sternkopf Lillebæk ◽  
Birgitte Haahr Kallipolitis
Keyword(s):  
Listeria Monocytogenes ◽  
Adhesion Protein ◽  
Listeria Adhesion Protein

scholarly journals N-Terminal Gly224–Gly411 Domain in Listeria Adhesion Protein Interacts with Host Receptor Hsp60

PLoS ONE ◽  
2011 ◽  
Vol 6 (6) ◽  
pp. e20694 ◽  
Author(s):  
Balamurugan Jagadeesan ◽  
Amy E. Fleishman Littlejohn ◽  
Mary Anne Roshni Amalaradjou ◽  
Atul K. Singh ◽  
Krishna K. Mishra ◽  
...  
Keyword(s):  
Adhesion Protein ◽  
Listeria Adhesion Protein
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