Directly Bactericidal Anti-Escherichia coli Antibody

2018 ◽  
Vol 26 (8) ◽  
pp. 642-644 ◽  
Author(s):  
Valéria Szijártó ◽  
Eszter Nagy ◽  
Gábor Nagy
Keyword(s):  
Escherichia Coli ◽  
Coli Antibody

scholarly journals Immunoglobulin and anti-Escherichia coli antibody in lower respiratory tract secretions from infants weighing less than 1500 g at birth.

1990 ◽  
Vol 65 (1 Spec No) ◽  
pp. 48-53 ◽  
Author(s):  
F H Sennhauser ◽  
A Balloch ◽  
M J Shelton ◽  
L W Doyle ◽  
V Y Yu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Respiratory Tract ◽  
Lower Respiratory Tract ◽  
Coli Antibody

THE PASSIVE TRANSFER OF ANTIBODIES TO ESCHERICHIA COLI 0111:B4 FROM MOTHER TO OFFSPRING

PEDIATRICS ◽  
1961 ◽  
Vol 27 (2) ◽  
pp. 308-313
Author(s):  
Sidney Sussman
Keyword(s):  
Escherichia Coli ◽  
Antibody Titer ◽  
Passive Transfer ◽  
The Third ◽  
Coli Antibody ◽  
Antibody Titers

Esch. coli antibody titers in 27 mothers and their respective offspring were studied by the trypsinated and nontrypsinated hemagglutination technic. All of the maternal sera and colostra contained Esch. coli 0111-B4 antibody. In 19 cases the antibody titer in the specimens of colostrum on the first day was higher than that of the corresponding sera. The antibody titer in the colstrum fell rapidly during the next 3 to 4 days. Five cord sera had a low antibody titer to Esch. coli 0111:B4 when tested by the trypsinated hemagglutination method. By contrast, only two cord sera were positive for Esch. coli 0111:B4 antibody when tested by the untrypsinated hemagglutination technic. With the trypsinated method, two infants showed a 2-tube rise and one infant had a 1-tube rise in titer at the end of the third colostrum day; one infant demonstrated a 1-tube rise in titer when tested by the untrypsinated hemagglutination technic. In general, there was a 1-to-3-tube difference between the trypsinated and untrypsinated hemagglutination procedures.

scholarly journals Relationship to coagglutination of immunoglobulin class dissociated from Escherichia coli-antibody complexes.

1980 ◽  
Vol 12 (4) ◽  
pp. 608-609 ◽  
Author(s):  
D L Hovanec ◽  
D R Absolom ◽  
C J van Oss ◽  
E A Gorzynski
Keyword(s):  
Escherichia Coli ◽  
Immunoglobulin Class ◽  
Coli Antibody

scholarly journals Latex Test for Quantitative Determination of Escherichia coli Antibody

1974 ◽  
Vol 28 (6) ◽  
pp. 1073-1075
Author(s):  
Karim Hechemy ◽  
Roy Stevens ◽  
Jean Sroka ◽  
Hassan Gaafar
Keyword(s):  
Escherichia Coli ◽  
Quantitative Determination ◽  
Coli Antibody ◽  
Latex Test

scholarly journals Latex Test for Quantitative Determination of Escherichia coli Antibody

1974 ◽  
Vol 28 (6) ◽  
pp. 1073-1075 ◽  
Author(s):  
Karim Hechemy ◽  
Roy Stevens ◽  
Jean Sroka ◽  
Hassan Gaafar
Keyword(s):  
Escherichia Coli ◽  
Quantitative Determination ◽  
Coli Antibody ◽  
Latex Test

scholarly journals Prognostic indicators of post partum viability of kids born to Escherichia coli-vaccinated or unvaccinated does : research communication

2000 ◽  
Vol 71 (1) ◽  
Author(s):  
S.J.M. Munyua ◽  
D.I. Karioki ◽  
D.M. Chibeu ◽  
J.K. Wabacha ◽  
A.G. Thaiya ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Body Weight ◽  
Blood Glucose ◽  
Antibody Titre ◽  
Total Protein ◽  
Post Partum ◽  
Prognostic Indicators ◽  
Male And Female ◽  
E Coli ◽  
Coli Antibody

This study was undertaken to determine some blood and other physiological parameters with potential for use as prognostic indicators of viability of newborn goat kids. Of the 143 kids born during the on-farmstudy, 97 were crosses of Galla × Small East African (SEA) and 46 were pure SEA. The SEA × Galla kids were 46 single males, with a mean body weight at birth of 2.77 ± 0.22 kg , 43 females with a mean body weight at birth of 2.36 ± 0.76 kg and 5 and 3 sets of female and male twins (mean body weight at birth of 1.8 ± 0.19 kg and 2.05 ± 0.07 kg for the female and male kids, respectively). The SEA kids comprised 36 single male and female kids (mean body weight at birth of 2.48 ± 0.04 kg and 10 sets of twins (both male and female) (mean body weight at birth of 1.50 ± 0.04 kg ). Pre-suckling sera obtained on-station from kids born of does vaccinated against Escherichia coli (n = 8) and unvaccinated does (n = 7) had a total protein content of < 40.0 g/l and no detectable levels of IgG and A or E. coli antibodies. Sera obtained 12 hours post partum from kids that survived in both groups contained about 19-22 g of Ig g/ , 50-80 g total protein/ , blood glucose of >5 mmol/l and had an E. coli antibody titre of between 1/160 and 1/640. On the other hand, kids that died within 48 hours of birth (parturient deaths) and had been classified in categories 3 and 4 righting reaction had low (< 40 g//l ) total protein, low white blood cell count (4000/m/l ) and low blood glucose concentration (< 4.9 mmol//l ). It is concluded that kids with delayed righting reaction (> 45 minutes), low rectal temperature (< 36 °C), low birth weights (< 1.5 kg for singles and < 1.0 kg for twins), low white blood cells (< 4000/ m/l ), low (< 2 mmol/l ) blood glucose levels, low total protein (< 40.0 g//l ), low (< 1:160) E. coli antibody titre and IgG ( < 3350 mg//l ) in sera obtained 12 hours after birth have a poor prognosis for survival.

scholarly journals Effectiveness of Sensors Contact Metallization (Ti, Au, and Ru) and Biofunctionalization for Escherichia coli Detection

Sensors ◽  
2018 ◽  
Vol 18 (9) ◽  
pp. 2912 ◽  
Author(s):  
Sabina Górska ◽  
Artur Rydosz ◽  
Ewa Brzozowska ◽  
Marek Drab ◽  
Krzysztof Wincza ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
High Frequency ◽  
Iminodiacetic Acid ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sensor Surface ◽  
Lipopolysaccharide Binding Protein ◽  
E Coli ◽  
Contact Metallization ◽  
Coli Antibody ◽  
Lipopolysaccharide Binding

In designing a bacteria biosensor, various issues must be addressed: the specificity of bacteria recognition, the immobilization of biomolecules that act as the bacteria receptor, and the selectivity of sensor surface. The aim of this paper was to examine how the biofunctionalized surface of Ti, Au, and Ru metals reacts in contact with strains of Escherichia coli (E. coli). The focus on metal surfaces results from their future use as electrodes in high frequency biosensors, e.g., resonant circuits or transmission-line sections. First, the surfaces of different metals were chemically functionalized with 3-aminopropyltriethoxysilane (APTES) and glutaraldehyde or with 3-glycidylooxypropyltrimethoxysilane (GPTMS) followed by N-(5-amino-1-carboxypentyl) iminodiacetic acid (AB-NTA) and NiCl2. Secondly, the lipopolysaccharide binding protein (LBP), polyclonal anti-Escherichia coli antibody and bacteriophage protein gp37 were tested as bacteria receptors. The selectivity and specificity have been confirmed by the Enzyme-Linked Immunosorbent Assay (ELISA) and visualized by scanning electron microscopy at low landing energies. We noticed that LBP, polyclonal antibody, and gp37 were successfully immobilized on all studied metals and recognized the E. coli bacteria selectively. However, for the antibody, the highest reactivity was observed when Ti surface was modified, whereas the bacteria binding was comparable between LBP and gp37 on the functionalized Ru surfaces, independent from modification. Thus, all surfaces were biocompatible within the scope of biosensor functionality, with titanium functionalization showing the best performance.

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