Growth factors and chondrogenic differentiation of mesenchymal stem cells

2012 ◽  
Vol 44 (2) ◽  
pp. 69-73 ◽  
Author(s):  
Ľ. Danišovič ◽  
I. Varga ◽  
Š. Polák
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factors ◽  
Chondrogenic Differentiation

Growth Factors Cross-Linked to Collagen Microcarriers Promote Expansion and Chondrogenic Differentiation of Human Mesenchymal Stem Cells

2015 ◽  
Vol 21 (19-20) ◽  
pp. 2618-2628 ◽  
Author(s):  
Alessandro Bertolo ◽  
Fanny Arcolino ◽  
Simona Capossela ◽  
Anna Rita Taddei ◽  
Martin Baur ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factors ◽  
Chondrogenic Differentiation ◽  
Human Mesenchymal Stem Cells

Comparative profiling of chondrogenic differentiation of mesenchymal stem cells (MSCs) driven by two different growth factors

2019 ◽  
Vol 37 (5) ◽  
pp. 359-367
Author(s):  
Xintang Zhan ◽  
Peian Cai ◽  
Danqing Lei ◽  
Yifeng Yang ◽  
Zetao Wang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factors ◽  
Chondrogenic Differentiation

The effect of non-growth factors on chondrogenic differentiation of mesenchymal stem cells

2013 ◽  
Vol 15 (3) ◽  
pp. 319-327 ◽  
Author(s):  
Xiujie Zhang ◽  
Yumin Zhang ◽  
Zhiqiang Wang ◽  
Qijia Li ◽  
Baoxing Li
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factors ◽  
Chondrogenic Differentiation

scholarly journals Effect of Activated Platelet-Rich Plasma on Chondrogenic Differentiation of Rabbit Bone Marrow-Derived Mesenchymal Stem Cells

2021 ◽  
Author(s):  
Zhen Wang ◽  
Zheng Wang ◽  
Bin Zhang ◽  
Qinghua Zhao ◽  
Yubao Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Growth Factors ◽  
Chondrogenic Differentiation ◽  
Platelet Rich Plasma ◽  
Cell Counting ◽  
Chondrogenic Medium ◽  
Tissue Repair And Regeneration ◽  
Rabbit Bone

Abstract Background Platelet-rich plasma (PRP) has revealed benefits in tissue repair and regeneration, however, the effect of PRP on proliferation and chondrogenesis of mesenchymal stem cells remains controversial.This study is to evaluate the effect of different concentration PRP on proliferation and chondrogenic differentiation of rabbit bone marrow-derived mesenchymal stem cells(BMSCs). Methods PRP was obtained by centrifugation and activation, in which growth factors and cytokines were detected. BMSCs were isolated from rabbit bone marrow and characterized by flow cytometry. About 5 × 103 BMSCs were cultured in high glucose Dulbecco’s modified Eagle’s medium (HG-DMEM) with 4 different compositions: 10% fetal bovine serum, 5%PRP, 10%PRP, and 15%PRP for consecutive 7 days. Cell counting assays were performed on days 1, 3, 5, and 7 to evaluate the BMSCs proliferation. In chondrogenic differentiation, high-density cell pellets composed of 5 × 105 BMSCs were induced in 4 conditions: commercial chondrogenic medium (control), 5%PRP (HG-DMEM + 5%PRP), 10%PRP (HG-DMEM + 10%PRP), and 15%PRP (HG-DMEM + 15%PRP) for 21 days. The gene expression levels of aggrecan (ACAN), collagen type Ⅱ Alpha 1 (COL2A1), and SRY-Box Transcription Factor 9 (SOX9) in pellets were detected. Histological assessments were performed by morphologically observation and pathological stain. Independent-samples t-test and one-way analysis of variance were used in statistical analyses. Results The concentrations of growth factors and cytokines were elevated in PRP. BMSCs proliferation was enhanced in all groups, and 10% PRP revealed more obvious outcome than the others from day 5. In chondrogenic differentiation, the levels of ACAN, COL2A1, and SOX9 were lower in 3 PRP groups than control, but ACAN and SOX9 was higher in 10% PRP group than 5% and 15%. Histological examinations showed 10% PRP-treated pellets showed more regular appearance, larger size, and abundant extracellular matrix than 5% and 10% groups, but still inferior to commercial chondrogenic medium. Conclusions PRP may enhance the proliferation of rabbit BMSCs, while with limited effect on chondrogenic differentiation compared with commercial chondrogenic medium in pellets culture. Whether optimizing and modifying PRP components would lead to satisfying chondrogenesis of BMSCs, it is deserved furthermore study.

Cell-Matrix Interactions Modulate Mesenchymal Stem Cell Response to Dynamic Compression

2011 ◽  
Author(s):  
Stephen D. Thorpe ◽  
Conor T. Buckley ◽  
Andrew J. Steward ◽  
Daniel J. Kelly
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Growth Factors ◽  
Chondrogenic Differentiation ◽  
Dynamic Compression ◽  
Compressive Loading ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Cell Matrix Interactions

Unconfined cyclic compressive loading has been shown to promote the chondrogenic differentiation of agarose encapsulated mesenchymal stem cells (MSCs) in the absence of chondrogenic growth factors [1, 2]. However, in general robust chondrogenesis has not been reported as a result of mechanical stimulation alone; with biochemical stimulation through TGF-β supplementation yielding a more potent pro-chondrogenic effect [2, 3].

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