scholarly journals CSE–MECC two-dimensional capillary electrophoresis analysis of proteins in the mouse tumor cell (AtT-20) homogenate

Talanta ◽  
2007 ◽  
Vol 71 (5) ◽  
pp. 1981-1985 ◽  
Author(s):  
X CHEN ◽  
M ABULFAZAL ◽  
N DOVICHI
Keyword(s):  
Capillary Electrophoresis ◽  
Tumor Cell ◽  
Mouse Tumor ◽  
Two Dimensional

scholarly journals Fully Automated Two-dimensional Capillary Electrophoresis for High Sensitivity Protein Analysis

2001 ◽  
Vol 1 (1) ◽  
pp. 69-74 ◽  
Author(s):  
David A. Michels ◽  
Shen Hu ◽  
Regine M. Schoenherr ◽  
Michael J. Eggertson ◽  
Norman J. Dovichi
Keyword(s):  
Capillary Electrophoresis ◽  
High Sensitivity ◽  
Protein Analysis ◽  
Two Dimensional

scholarly journals Amplicon structure in multidrug-resistant murine cells: a nonrearranged region of genomic DNA corresponding to large circular DNA.

1992 ◽  
Vol 12 (3) ◽  
pp. 1179-1187 ◽  
Author(s):  
F Ståhl ◽  
Y Wettergren ◽  
G Levan
Keyword(s):  
Tumor Cell ◽  
Cell Line ◽  
Single Unit ◽  
Parental Cell ◽  
Parental Cell Line ◽  
Mouse Tumor ◽  
Dna Molecules ◽  
Circular Dna ◽  
Double Minutes ◽  
Mdr Genes

Multidrug resistance (MDR) in tumor cell lines is frequently correlated with amplification of one or more mdr genes. Usually the amplified domain also includes several neighboring genes. Using pulsed-field gel electrophoresis, we have established a restriction map covering approximately 2,200 kb in the drug-sensitive mouse tumor cell line TC13K. The mapped region is located on mouse chromosome 5 and includes the three mdr genes, the gene for the calcium-binding sorcin protein, and a gene with unknown function designated class 5. Long-range maps of the amplified DNA sequences in five of six MDR sublines that had been independently derived from TC13K generally displayed the same pattern as did the parental cell line. All six MDR sublines exhibited numerous double minutes, and one of them displayed a homogeneously staining region in a subpopulation. Large circular molecules, most likely identical to one chromatid of the double minutes, were detected in four of the sublines by linearization with gamma irradiation. The size of the circles was about 2,500 kb, which correlated to a single unit of the amplified domain. We therefore propose that in four independent instances of MDR development, a single unit of about 2,500 kb has been amplified in the form of circular DNA molecules. The restriction enzyme map of the amplified unit is unchanged compared with that of the parental cell line, whereas the joining sites of the circular DNA molecules are not identical but are in the same region.

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