Oxidative stress is involved in Dasatinib-induced apoptosis in rat primary hepatocytes

2012 ◽  
Vol 261 (3) ◽  
pp. 280-291 ◽  
Author(s):  
Tao Xue ◽  
Peihua Luo ◽  
Hong Zhu ◽  
Yuqin Zhao ◽  
Honghai Wu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Primary Hepatocytes ◽  
Rat Primary Hepatocytes ◽  
Induced Apoptosis

Cadmium induced apoptosis in mouse primary hepatocytes: the role of oxidative stress-mediated ERK pathway activation and the involvement of histone H3 phosphorylation

RSC Advances ◽  
2015 ◽  
Vol 5 (40) ◽  
pp. 31798-31806 ◽  
Author(s):  
Jing Wang ◽  
Minglu Hao ◽  
Chunguang Liu ◽  
Rutao Liu
Keyword(s):  
Oxidative Stress ◽  
Histone H3 ◽  
Erk Signaling ◽  
Erk Pathway ◽  
Primary Hepatocytes ◽  
H3 Phosphorylation ◽  
Pathway Activation ◽  
Induced Apoptosis ◽  
Histone H3 Phosphorylation

Time-delayed apoptosis induced by cadmium in primary hepatocytes through DNA damage, histone modification and ERK signaling cascade, which are all mediated by oxidative stress.

Tetrandrine-induced apoptosis in rat primary hepatocytes is initiated from mitochondria: Caspases and Endonuclease G (Endo G) pathway

Toxicology ◽  
2006 ◽  
Vol 218 (1) ◽  
pp. 1-12 ◽  
Author(s):  
C YAN ◽  
Q XINMING ◽  
G LIKUN ◽  
L LINLIN ◽  
C FANGPING ◽  
...  
Keyword(s):  
Primary Hepatocytes ◽  
Endonuclease G ◽  
Rat Primary Hepatocytes ◽  
Induced Apoptosis

Protective effects of dried flower extracts of Hibiscus sabdariffa L. against oxidative stress in rat primary hepatocytes

1997 ◽  
Vol 35 (12) ◽  
pp. 1159-1164 ◽  
Author(s):  
T.-H. Tseng ◽  
E.-S. Kao ◽  
C.-Y. Chu ◽  
F.-P. Chou ◽  
H.-W. Lin Wu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hibiscus Sabdariffa ◽  
Protective Effects ◽  
Primary Hepatocytes ◽  
Rat Primary Hepatocytes

scholarly journals Investigation of sphingosin-1-phosphate-triggered matriptase activation using a rat primary hepatocyte model

2019 ◽  
Vol 67 (4) ◽  
pp. 578-587
Author(s):  
Réka Fanni Barna ◽  
Judit Mercédesz Pomothy ◽  
Zsuzsanna Paréj ◽  
Erzsébet Pásztiné Gere
Keyword(s):  
Oxidative Stress ◽  
Cell Death ◽  
Cell Viability ◽  
Mechanism Of Action ◽  
Sandwich Elisa ◽  
Colorimetric Method ◽  
Redox Status ◽  
Primary Hepatocytes ◽  
Rat Primary Hepatocytes ◽  
Sphingosine 1 Phosphate

Sphingosine-1-phosphate (S1P) has been reported as a matriptase activator. The aim of this study was to reveal if S1P can influence hepcidin production. Furthermore, we investigated how S1P can affect the viability and the redox status of primary hepatocytes. Rat primary hepatocytes were cultivated for 72 h and were treated with 50, 200, 1000 ng/ml S1P. Cell-free supernatants were collected every 24 h. Cell viability was tested by a colorimetric method using tetrazolium compound (MTS). The hepcidin levels in the cell-free supernatants were examined with hepcidin sandwich ELISA to determine the effect of S1P on the hepcidin-modulating ability of matriptase. In order to estimate the extent of S1P-generated oxidative stress, extracellular H2O2 measurements were performed by the use of fluorescent dye. Based on the findings, S1P treatment did not cause cell death for 72 h at concentrations up to 1000 ng/ml. S1P did not influence the extracellular H2O2 production for 72 h. The hepcidin levels were significantly suppressed in hepatocytes exposed to S1P treatment. Further studies would be needed to explore the exact mechanism of action of S1P.

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