Sequential high-pressure extraction to obtain capsinoids and phenolic compounds from biquinho pepper (Capsicum chinense)

2019 ◽  
Vol 150 ◽  
pp. 112-121 ◽  
Author(s):  
Ana Carolina de Aguiar ◽  
Ana Paula da Fonseca Machado ◽  
Célio Fernando Figueiredo Angolini ◽  
Damila Rodrigues de Morais ◽  
Andressa Mara Baseggio ◽  
...  
Antioxidants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1469 ◽  
Author(s):  
Mário Bordalo ◽  
Inês J. Seabra ◽  
Andreia Bento Silva ◽  
Ana Paula Terrasso ◽  
Catarina Brito ◽  
...  

Bravo de Esmolfe (BE) is a traditional Portuguese apple highly appreciated by consumers due to its peculiar flavor and aroma. This apple contains higher concentration of phenolic compounds than other cultivars and is thus considered a rich source of antioxidants. Its sensorial and functional properties have attracted farmers’ associations to increase BE production. However, a large quantity of apples is wasted due to storage/transportation procedures that impact BE’s quality attributes. In this work, we applied high-pressure extraction methodologies to generate antioxidant-rich fractions from BE residues aiming at adding high value to these agro-food by-products. We performed a first extraction step using supercritical CO2, followed by a second extraction step where different CO2 + ethanol mixtures (10–100% v/v) were tested. All experiments were carried out at 25 MPa and 50 °C. Extracts were characterized in terms of global yield, phenolic content and antioxidant activity using chemical (ORAC, HOSC, HORAC) and cell-based assays (CAA). We demonstrated that, although the pressurized 100% ethanol condition promoted the highest recovery of phenolic compounds (509 ± 8 mg GAE/100 g BE residues), the extract obtained with 40% ethanol presented the highest CAA (1.50 ± 0.24 µmol QE/g dw) and ORAC (285 ± 16 µmol TEAC/g dw), as well as HOSC and HORAC values, which correlated with its content of epicatechin and procyanidin B2. Noteworthy, this fraction inhibited free radical production in human neurospheroids derived from NT2 cells, a robust 3D cell model for neuroprotective testing.


2014 ◽  
Vol 34 (4) ◽  
pp. 447-451 ◽  
Author(s):  
R. Casquete ◽  
S.M. Castro ◽  
M.C. Villalobos ◽  
M.J. Serradilla ◽  
R.P. Queirós ◽  
...  

2009 ◽  
Vol 38 (9) ◽  
pp. 1243-1252 ◽  
Author(s):  
Myoung-Hoon Jeong ◽  
Seung-Seop Kim ◽  
Ji-Hye Ha ◽  
Ling Jin ◽  
Hak-Ju Lee ◽  
...  

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