scholarly journals Ligand-Release Pathways in the Pheromone-Binding Protein of Bombyx mori

Structure ◽  
2006 ◽  
Vol 14 (10) ◽  
pp. 1567-1576 ◽  
Author(s):  
Frauke Gräter ◽  
Bert L. de Groot ◽  
Hualiang Jiang ◽  
Helmut Grubmüller
Keyword(s):  
Bombyx Mori ◽  
Binding Protein ◽  
Pheromone Binding Protein

scholarly journals Responses of the Pheromone-Binding Protein of the Silk Moth Bombyx mori on a Graphene Biosensor Match Binding Constants in Solution

Sensors ◽  
2021 ◽  
Vol 21 (2) ◽  
pp. 499
Author(s):  
Caroline Bonazza ◽  
Jiao Zhu ◽  
Roger Hasler ◽  
Rosa Mastrogiacomo ◽  
Paolo Pelosi ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Binding Protein ◽  
Binding Constants ◽  
Isoamyl Acetate ◽  
The Other ◽  
Oxide Surface ◽  
Binding Assays ◽  
Pheromone Binding Protein ◽  
Silk Moth ◽  
Good Agreement

An electronic biosensor for odors was assembled by immobilizing the silk moth Bombyx mori pheromone binding protein (BmorPBP1) on a reduced graphene oxide surface of a field-effect transistor. At physiological pH, the sensor detects the B. mori pheromones, bombykol and bombykal, with good affinity and specificity. Among the other odorants tested, only eugenol elicited a strong signal, while terpenoids and other odorants (linalool, geraniol, isoamyl acetate, and 2-isobutyl-3-methoxypyrazine) produced only very weak responses. Parallel binding assays were performed with the same protein and the same ligands, using the common fluorescence approach adopted for similar proteins. The results are in good agreement with the sensor’s responses: bombykol and bombykal, together with eugenol, proved to be strong ligands, while the other compounds showed only poor affinity. When tested at pH 4, the protein failed to bind bombykol both in solution and when immobilized on the sensor. This result further indicates that the BmorPBP1 retains its full activity when immobilized on a surface, including the conformational change observed in acidic conditions. The good agreement between fluorescence assays and sensor responses suggests that ligand-binding assays in solution can be used to screen mutants of a binding protein when selecting the best form to be immobilized on a biosensor.

Disulfide structure of the pheromone binding protein from the silkworm moth, Bombyx mori

FEBS Letters ◽  
1999 ◽  
Vol 464 (1-2) ◽  
pp. 85-90 ◽  
Author(s):  
Walter S Leal ◽  
Larisa Nikonova ◽  
Guihong Peng
Keyword(s):  
Bombyx Mori ◽  
Binding Protein ◽  
Pheromone Binding Protein ◽  
Disulfide Structure

Synthesis of an immobilized Bombyx mori pheromone-binding protein liquid chromatography stationary phase

Talanta ◽  
2006 ◽  
Vol 70 (4) ◽  
pp. 752-755 ◽  
Author(s):  
Armenak Margaryan ◽  
Ruin Moaddel ◽  
Jeffrey R. Aldrich ◽  
Jennifer M. Tsuruda ◽  
Angela M. Chen ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phase ◽  
Bombyx Mori ◽  
Binding Protein ◽  
Pheromone Binding Protein

scholarly journals Pheromone discrimination by a pH-tuned polymorphism of the Bombyx mori pheromone-binding protein

2013 ◽  
Vol 110 (46) ◽  
pp. 18680-18685 ◽  
Author(s):  
F. F. Damberger ◽  
E. Michel ◽  
Y. Ishida ◽  
W. S. Leal ◽  
K. Wuthrich
Keyword(s):  
Bombyx Mori ◽  
Binding Protein ◽  
Pheromone Binding Protein

Dynamic Conformational Equilibria in the Physiological Function of the Bombyx mori Pheromone-Binding Protein

2011 ◽  
Vol 408 (5) ◽  
pp. 922-931 ◽  
Author(s):  
Erich Michel ◽  
Fred F. Damberger ◽  
Yuko Ishida ◽  
Francesco Fiorito ◽  
Donghan Lee ◽  
...  
Keyword(s):  
Bombyx Mori ◽  
Physiological Function ◽  
Binding Protein ◽  
Pheromone Binding Protein ◽  
Conformational Equilibria

Coil-to-helix transition and ligand release of Bombyx mori pheromone-binding protein

2005 ◽  
Vol 335 (4) ◽  
pp. 1044-1050 ◽  
Author(s):  
Catherine Lautenschlager ◽  
Walter S. Leal ◽  
Jon Clardy
Keyword(s):  
Bombyx Mori ◽  
Binding Protein ◽  
Pheromone Binding Protein

scholarly journals Bombyx mori Pheromone-Binding Protein Binding Nonpheromone Ligands: Implications for Pheromone Recognition

Structure ◽  
2007 ◽  
Vol 15 (9) ◽  
pp. 1148-1154 ◽  
Author(s):  
Catherine Lautenschlager ◽  
Walter S. Leal ◽  
Jon Clardy
Keyword(s):  
Protein Binding ◽  
Bombyx Mori ◽  
Binding Protein ◽  
Pheromone Binding Protein

Deciphering the Selectivity of Bombyx mori Pheromone Binding Protein for Bombykol over Bombykal: A Theoretical Approach

ChemPhysChem ◽  
2008 ◽  
Vol 9 (18) ◽  
pp. 2785-2793 ◽  
Author(s):  
Landry Charlier ◽  
Serge Antonczak ◽  
Emmanuelle Jacquin-Joly ◽  
Daniel Cabrol-Bass ◽  
Jérôme Golebiowski
Keyword(s):  
Bombyx Mori ◽  
Theoretical Approach ◽  
Binding Protein ◽  
Pheromone Binding Protein
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