scholarly journals Regulation of the DNA Methylation Landscape in Human Somatic Cell Reprogramming by the miR-29 Family

2016 ◽  
Vol 7 (1) ◽  
pp. 43-54 ◽  
Author(s):  
Eriona Hysolli ◽  
Yoshiaki Tanaka ◽  
Juan Su ◽  
Kun-Yong Kim ◽  
Tianyu Zhong ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Somatic Cell ◽  
Cell Reprogramming ◽  
Human Somatic Cell ◽  
Somatic Cell Reprogramming

scholarly journals X Chromosome of Female Cells Shows Dynamic Changes in Status during Human Somatic Cell Reprogramming

2014 ◽  
Vol 2 (6) ◽  
pp. 896-909 ◽  
Author(s):  
Kun-Yong Kim ◽  
Eriona Hysolli ◽  
Yoshiaki Tanaka ◽  
Brandon Wang ◽  
Yong-Wook Jung ◽  
...  
Keyword(s):  
Somatic Cell ◽  
X Chromosome ◽  
Cell Reprogramming ◽  
Dynamic Changes ◽  
Human Somatic Cell ◽  
Somatic Cell Reprogramming

scholarly journals RNAi Reveals Phase-Specific Global Regulators of Human Somatic Cell Reprogramming

Cell Reports ◽  
2016 ◽  
Vol 15 (12) ◽  
pp. 2597-2607 ◽  
Author(s):  
Cheng-Xu Delon Toh ◽  
Jun-Wei Chan ◽  
Zheng-Shan Chong ◽  
Hao Fei Wang ◽  
Hong Chao Guo ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Reprogramming ◽  
Global Regulators ◽  
Human Somatic Cell ◽  
Somatic Cell Reprogramming

scholarly journals Transcriptome Signature and Regulation in Human Somatic Cell Reprogramming

2015 ◽  
Vol 4 (6) ◽  
pp. 1125-1139 ◽  
Author(s):  
Yoshiaki Tanaka ◽  
Eriona Hysolli ◽  
Juan Su ◽  
Yangfei Xiang ◽  
Kun-Yong Kim ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Reprogramming ◽  
Human Somatic Cell ◽  
Somatic Cell Reprogramming ◽  
Transcriptome Signature

scholarly journals Enhanced human somatic cell reprogramming efficiency by fusion of the MYC transactivation domain and OCT4

2017 ◽  
Vol 25 ◽  
pp. 88-97 ◽  
Author(s):  
Ling Wang ◽  
Delun Huang ◽  
Chang Huang ◽  
Yexuan Yin ◽  
Kaneha Vali ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Reprogramming ◽  
Transactivation Domain ◽  
Human Somatic Cell ◽  
Somatic Cell Reprogramming ◽  
Reprogramming Efficiency

AP-1 activity is a major barrier of human somatic cell reprogramming

2021 ◽  
Author(s):  
Yuting Liu ◽  
Jiangping He ◽  
Ruhai Chen ◽  
He Liu ◽  
Jocelyn Chen ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Reprogramming ◽  
Major Barrier ◽  
Human Somatic Cell ◽  
Somatic Cell Reprogramming

scholarly journals An Efficient Chemical-Defined Condition for Generation of Human Induced Pluripotent Stem Cells

2021 ◽  
Author(s):  
Xu Jinhong ◽  
Fang Shi ◽  
Wang Naweng ◽  
Li Bo ◽  
Huang Yongheng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Small Molecules ◽  
Somatic Cell ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Cell Reprogramming ◽  
Human Somatic Cell ◽  
Somatic Cell Reprogramming ◽  
Induced Pluripotent

Abstract Background: Human induced pluripotent stem cells (hiPSCs) hold great potential in disease modeling, drug screening and cell therapy. However, efficiency and costs of hiPSCs preparation still need to be improved.Methods: We screened the compounds that target signaling pathways, epigenetic modifications or metabolic-process regulation to replace the growth factors. After small molecules treatment, TRA-1-60 staining was performed to quantify the efficiency of somatic cell reprogramming. Next, small molecule cocktail induced ESCs or iPSCs were examined with pluripotent markers expression. Finally, Genome-wide gene expression profile was then analyzed by RNA-seq to illustrate the mechanism of human somatic cell reprogramming. Result: Here, we found that a dual-specificity tyrosine phosphorylation-regulated kinase inhibitor ID-8 robustly enhanced human somatic cell reprogramming by upregulation of PDK4 and activation of glycolysis. Furthermore, we identified a novel growth-factor-free hiPSC generation system using small molecules ID-8/Kartogenin (IK). Finally, we developed IK medium combined with Low-dose bFGF to support the long-term expansion of human pluripotent stem cells. IK-iPSCs showed pluripotency and normal karyotype. Conclusions: Our studies may provide a novel growth-factor-free culture system to facilitate the generation of hiPSCs for multiple application in regenerative medicine.

scholarly journals Tracking and Predicting Human Somatic Cell Reprogramming Using Nuclear Characteristics

2020 ◽  
Vol 118 (9) ◽  
pp. 2086-2102 ◽  
Author(s):  
Kaivalya Molugu ◽  
Ty Harkness ◽  
Jared Carlson-Stevermer ◽  
Ryan Prestil ◽  
Nicole J. Piscopo ◽  
...  
Keyword(s):  
Somatic Cell ◽  
Cell Reprogramming ◽  
Human Somatic Cell ◽  
Somatic Cell Reprogramming
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