scholarly journals Cyclic AMP Signaling through Epac Axis Modulates Human Hemogenic Endothelium and Enhances Hematopoietic Cell Generation

2016 ◽  
Vol 6 (5) ◽  
pp. 692-703 ◽  
Author(s):  
Shobhit Saxena ◽  
Roger E. Rönn ◽  
Carolina Guibentif ◽  
Roksana Moraghebi ◽  
Niels-Bjarne Woods
Keyword(s):  
Cyclic Amp ◽  
Hematopoietic Cell ◽  
Cell Generation ◽  
Hemogenic Endothelium ◽  
Cyclic Amp Signaling

Elucidating cyclic AMP signaling in subcellular domains with optogenetic tools and fluorescent biosensors

2019 ◽  
Vol 47 (6) ◽  
pp. 1733-1747 ◽  
Author(s):  
Christina Klausen ◽  
Fabian Kaiser ◽  
Birthe Stüven ◽  
Jan N. Hansen ◽  
Dagmar Wachten
Keyword(s):  
Cyclic Amp ◽  
Adenosine Monophosphate ◽  
Adenylyl Cyclases ◽  
Temporal Precision ◽  
Fluorescent Biosensors ◽  
Subcellular Domains ◽  
Spatio Temporal ◽  
Hydrolysis Of ◽  
Shed Light ◽  
Cyclic Amp Signaling

The second messenger 3′,5′-cyclic nucleoside adenosine monophosphate (cAMP) plays a key role in signal transduction across prokaryotes and eukaryotes. Cyclic AMP signaling is compartmentalized into microdomains to fulfil specific functions. To define the function of cAMP within these microdomains, signaling needs to be analyzed with spatio-temporal precision. To this end, optogenetic approaches and genetically encoded fluorescent biosensors are particularly well suited. Synthesis and hydrolysis of cAMP can be directly manipulated by photoactivated adenylyl cyclases (PACs) and light-regulated phosphodiesterases (PDEs), respectively. In addition, many biosensors have been designed to spatially and temporarily resolve cAMP dynamics in the cell. This review provides an overview about optogenetic tools and biosensors to shed light on the subcellular organization of cAMP signaling.

CYCLIC AMP SIGNALING AND GENE REGULATION

1998 ◽  
Vol 18 (1) ◽  
pp. 353-383 ◽  
Author(s):  
Philip B. Daniel ◽  
William H. Walker ◽  
Joel F. Habener
Keyword(s):  
Gene Regulation ◽  
Cyclic Amp ◽  
Cyclic Amp Signaling
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