scholarly journals Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State

2020 ◽  
Vol 27 (5) ◽  
pp. 784-797.e11
Author(s):  
Liang Zhang ◽  
John Avery ◽  
Amelia Yin ◽  
Amar M. Singh ◽  
Timothy S. Cliff ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Paraxial Mesoderm ◽  
Brown Adipocytes

scholarly journals Production of Functional Classical Brown Adipocytes from Human Pluripotent Stem Cells using Specific Hemopoietin Cocktail without Gene Transfer

2012 ◽  
Vol 16 (5) ◽  
pp. 684-685 ◽  
Author(s):  
Miwako Nishio ◽  
Takeshi Yoneshiro ◽  
Masako Nakahara ◽  
Shinnosuke Suzuki ◽  
Koichi Saeki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Transfer ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Brown Adipocytes

scholarly journals Production of Functional Classical Brown Adipocytes from Human Pluripotent Stem Cells using Specific Hemopoietin Cocktail without Gene Transfer

2012 ◽  
Vol 16 (3) ◽  
pp. 394-406 ◽  
Author(s):  
Miwako Nishio ◽  
Takeshi Yoneshiro ◽  
Masako Nakahara ◽  
Shinnosuke Suzuki ◽  
Koichi Saeki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Gene Transfer ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Brown Adipocytes

scholarly journals Exogenous Cytokine-Free Differentiation of Human Pluripotent Stem Cells into Classical Brown Adipocytes

Cells ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 373 ◽  
Author(s):  
Oka ◽  
Kobayashi ◽  
Matsumura ◽  
Nishio ◽  
Saeki
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Pancreatic Beta Cells ◽  
Developmental Process ◽  
Human Pluripotent Stem Cells ◽  
Directed Differentiation ◽  
Brown Adipocytes ◽  
Exogenous Cytokine ◽  
Physiologically Active Substances ◽  
Adrenergic Receptor Agonist

We previously established a method for a directed differentiation of human pluripotent stem cells into classical brown adipocytes (BA) by forming aggregates via massive floating culture in the presence of a specific cytokine cocktail. However, use of recombinant cytokines requires significant cost. Moreover, an enforced differentiation by exogenously added cytokines may amend skewed differentiation propensity of patient’s pluripotent stem cells, providing unsatisfactory disease models. Therefore, an exogenous cytokine-free method, where cytokines required for differentiation are provided in an auto/paracrine manner mimicking natural developmental process, is beneficial. Here we show that, if human pluripotent stem cells are cultured as size-controlled spheroids (100–120 µm radius, 2000–2500 cells/spheroid) in a mutually segregated manner with half-change of the medium every other day, they differentiate into classical BA via an authentic MYF5-positive myoblast route in the absence of exogenous cytokines. Differentiated BA exerted thermogenic activity in transplanted mice in response to beta-adrenergic receptor agonist stimuli. The cytokine-free differentiation method has further advantages in exploring BATokines, BA-derived physiologically active substances. Indeed, we have found that BA produces an unknown small (<1000 Da), highly hydrophilic molecule that augments insulin secretion from pancreatic beta cells. Our upgraded technique will contribute to an advancement of stem cell study for diverse purposes.

scholarly journals Programming human pluripotent stem cells into white and brown adipocytes

2012 ◽  
Vol 14 (2) ◽  
pp. 209-219 ◽  
Author(s):  
Tim Ahfeldt ◽  
Robert T. Schinzel ◽  
Youn-Kyoung Lee ◽  
David Hendrickson ◽  
Adam Kaplan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Brown Adipocytes

Differentiation of human pluripotent stem cells into airway epithelial cells

Pneumologie ◽  
2015 ◽  
Vol 69 (07) ◽  
Author(s):  
S Ulrich ◽  
S Weinreich ◽  
R Haller ◽  
S Menke ◽  
R Olmer ◽  
...  
Keyword(s):  
Stem Cells ◽  
Epithelial Cells ◽  
Pluripotent Stem Cells ◽  
Airway Epithelial Cells ◽  
Human Pluripotent Stem Cells ◽  
Airway Epithelial

326-LB: BCL-xL/BCL2L1 Is a Critical Anti-Apoptotic Protein that Suppresses BAK to Promote Pancreatic Specification from Human Pluripotent Stem Cells

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 326-LB
Author(s):  
LARRY SAI WENG LOO ◽  
ADRIAN TEO ◽  
SOUMITA GHOSH ◽  
ANDREAS ALVIN PURNOMO SOETEDJO ◽  
LINH NGUYEN ◽  
...  
Keyword(s):  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Apoptotic Protein
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