scholarly journals Generation of Functional Liver Sinusoidal Endothelial Cells from Human Pluripotent Stem-Cell-Derived Venous Angioblasts

2020 ◽  
Vol 27 (2) ◽  
pp. 254-269.e9
Author(s):  
Blair K. Gage ◽  
Jeff C. Liu ◽  
Brendan T. Innes ◽  
Sonya A. MacParland ◽  
Ian D. McGilvray ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell ◽  
Liver Sinusoidal Endothelial Cells ◽  
Sinusoidal Endothelial Cells

scholarly journals A Genome-wide Analysis of Human Pluripotent Stem Cell-Derived Endothelial Cells in 2D or 3D Culture

2017 ◽  
Vol 8 (4) ◽  
pp. 907-918 ◽  
Author(s):  
Jue Zhang ◽  
Michael P. Schwartz ◽  
Zhonggang Hou ◽  
Yongsheng Bai ◽  
Hamisha Ardalani ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
3D Culture ◽  
Human Pluripotent Stem Cell ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
A Genome

scholarly journals Human pluripotent stem cell-derived epicardial progenitors can differentiate to endocardial-like endothelial cells

2017 ◽  
Vol 2 (2) ◽  
pp. 191-201 ◽  
Author(s):  
Xiaoping Bao ◽  
Vijesh J. Bhute ◽  
Tianxiao Han ◽  
Tongcheng Qian ◽  
Xiaojun Lian ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell

scholarly journals PU.1 drives specification of pluripotent stem cell-derived endothelial cells to LSEC-like cells

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Jonathan De Smedt ◽  
Elise Anne van Os ◽  
Irene Talon ◽  
Sreya Ghosh ◽  
Burak Toprakhisar ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Cell Lineage ◽  
Specific Protein ◽  
Immune Functions ◽  
Protein Markers ◽  
Liver Sinusoidal Endothelial Cells ◽  
Lineage Specification

AbstractTo date, there is no representative in vitro model for liver sinusoidal endothelial cells (LSECs), as primary LSECs dedifferentiate very fast in culture and no combination of cytokines or growth factors can induce an LSEC fate in (pluripotent stem cell (PSC)-derived) endothelial cells (ECs). Furthermore, the transcriptional programmes driving an LSEC fate have not yet been described. Here, we first present a computational workflow (CenTFinder) that can identify transcription factors (TFs) that are crucial for modulating pathways involved in cell lineage specification. Using CenTFinder, we identified several novel LSEC-specific protein markers, such as FCN2 and FCN3, which were validated by analysis of previously published single-cell RNAseq data. We also identified PU.1 (encoded by the SPI1 gene) as a major regulator of LSEC-specific immune functions. We show that SPI1 overexpression (combined with the general EC TF ETV2) in human PSCs induces ECs with an LSEC-like phenotype. The ETV2-SPI1-ECs display increased expression of LSEC markers, such as CD32B and MRC1, as well as several of the proposed novel markers. More importantly, ETV2-SPI1-ECs acquire LSEC functions, including uptake of FSA-FITC, as well as labelled IgG. In conclusion, we present the CenTFinder computational tool to identify key regulatory TFs within specific pathways, in this work pathways of lineage specification, and we demonstrate its use by the identification and validation of PU.1 as a master regulator for LSEC fating.

scholarly journals Enhanced Therapeutic and Long-Term Dynamic Vascularization Effects of Human Pluripotent Stem Cell–Derived Endothelial Cells Encapsulated in a Nanomatrix Gel

Circulation ◽  
2017 ◽  
Vol 136 (20) ◽  
pp. 1939-1954 ◽  
Author(s):  
Shin-Jeong Lee ◽  
Young-Doug Sohn ◽  
Adinarayana Andukuri ◽  
Sangsung Kim ◽  
Jaemin Byun ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Pluripotent Stem Cell ◽  
Human Pluripotent Stem Cell
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