scholarly journals Human iPSC Glial Mouse Chimeras Reveal Glial Contributions to Schizophrenia

2017 ◽  
Vol 21 (2) ◽  
pp. 195-208.e6 ◽  
Author(s):  
Martha S. Windrem ◽  
Mikhail Osipovitch ◽  
Zhengshan Liu ◽  
Janna Bates ◽  
Devin Chandler-Militello ◽  
...  
Keyword(s):  
Mouse Chimeras ◽  
Human Ipsc

B15 Human ipsc-mouse chimeras to study huntington’s disease phenotypes

2018 ◽  
Author(s):  
Josep M Canals ◽  
Andres Miguez ◽  
Phil Sanders ◽  
Georgina Bombau ◽  
Cristina Vila
Keyword(s):  
Huntington's Disease ◽  
Huntington’S Disease ◽  
Disease Phenotypes ◽  
Mouse Chimeras ◽  
Human Ipsc

scholarly journals Rapid 3D BioPrinting of a human iPSC-derived cardiac micro-tissue for high-throughput drug testing

2021 ◽  
Vol 3 ◽  
pp. 100007
Author(s):  
Kathleen L. Miller ◽  
Yi Xiang ◽  
Claire Yu ◽  
Jacob Pustelnik ◽  
Jerry Wu ◽  
...  
Keyword(s):  
High Throughput ◽  
Drug Testing ◽  
3D Bioprinting ◽  
Human Ipsc

scholarly journals An Efficient Method for the Differentiation of Human iPSC-Derived Endoderm toward Enterocytes and Hepatocytes

Cells ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 812
Author(s):  
Shimeng Qiu ◽  
Yaling Li ◽  
Yuki Imakura ◽  
Shinji Mima ◽  
Tadahiro Hashita ◽  
...  
Keyword(s):  
Small Intestine ◽  
Activin A ◽  
Double Positive ◽  
Differentiation Method ◽  
Drug Metabolizing Enzyme ◽  
Human Ipscs ◽  
Novel Method ◽  
Induced Pluripotent ◽  
Metabolizing Enzyme ◽  
Human Ipsc

The endoderm, differentiated from human induced pluripotent stem cells (iPSCs), can differentiate into the small intestine and liver, which are vital for drug absorption and metabolism. The development of human iPSC-derived enterocytes (HiEnts) and hepatocytes (HiHeps) has been reported. However, pharmacokinetic function-deficiency of these cells remains to be elucidated. Here, we aimed to develop an efficient differentiation method to induce endoderm formation from human iPSCs. Cells treated with activin A for 168 h expressed higher levels of endodermal genes than those treated for 72 h. Using activin A (days 0–7), CHIR99021 and PI−103 (days 0–2), and FGF2 (days 3–7), the hiPSC-derived endoderm (HiEnd) showed 97.97% CD−117 and CD−184 double-positive cells. Moreover, HiEnts derived from the human iPSC line Windy had similar or higher expression of small intestine-specific genes than adult human small intestine. Activities of the drug transporter P-glycoprotein and drug-metabolizing enzyme cytochrome P450 (CYP) 3A4/5 were confirmed. Additionally, Windy-derived HiHeps expressed higher levels of hepatocyte- and pharmacokinetics-related genes and proteins and showed higher CYP3A4/5 activity than those derived through the conventional differentiation method. Thus, using this novel method, the differentiated HiEnts and HiHeps with pharmacokinetic functions could be used for drug development.

scholarly journals Establishment of MHHi001-A-5, a GCaMP6f and RedStar dual reporter human iPSC line for in vitro and in vivo characterization and in situ tracing of iPSC derivatives

2021 ◽  
Vol 52 ◽  
pp. 102206
Author(s):  
Alexandra Haase ◽  
Tim Kohrn ◽  
Veronika Fricke ◽  
Maria Elena Ricci Signorini ◽  
Merlin Witte ◽  
...  
Keyword(s):  
Ipsc Line ◽  
Dual Reporter ◽  
In Vivo Characterization ◽  
Human Ipsc

Culture Variabilities of Human iPSC-Derived Cerebral Organoids Are a Major Issue for the Modelling of Phenotypes Observed in Alzheimer’s Disease

2021 ◽  
Author(s):  
Damián Hernández ◽  
Louise A. Rooney ◽  
Maciej Daniszewski ◽  
Lerna Gulluyan ◽  
Helena H. Liang ◽  
...  
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Human Ipsc

scholarly journals Generation and characterization of a human iPSC line SANi006-A from a Gray Platelet Syndrome patient

2021 ◽  
pp. 102443
Author(s):  
Cathelijn E.M. Aarts ◽  
Eszter Varga ◽  
Steven Webbers ◽  
Judy Geissler ◽  
Marieke von Lindern ◽  
...  
Keyword(s):  
Syndrome Patient ◽  
Ipsc Line ◽  
Gray Platelet Syndrome ◽  
Human Ipsc
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