scholarly journals PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs

2017 ◽  
Vol 20 (2) ◽  
pp. 274-289.e7 ◽  
Author(s):  
Zhexin Zhu ◽  
Chunliang Li ◽  
Yanwu Zeng ◽  
Jianyi Ding ◽  
Zepeng Qu ◽  
...  
Keyword(s):  
Human Escs ◽  
Metabolic Circuit

scholarly journals PHB Associates with the HIRA Complex to Control an Epigenetic-Metabolic Circuit in Human ESCs

2020 ◽  
Vol 26 (2) ◽  
pp. 294
Author(s):  
Zhexin Zhu ◽  
Chunliang Li ◽  
Yanwu Zeng ◽  
Jianyi Ding ◽  
Zepeng Qu ◽  
...  
Keyword(s):  
Human Escs ◽  
Metabolic Circuit

scholarly journals The Autophagy Gene Atg16L1 is Necessary for Endometrial Decidualization

Endocrinology ◽  
2019 ◽  
Vol 161 (1) ◽  
Author(s):  
Arin K Oestreich ◽  
Sangappa B Chadchan ◽  
Pooja Popli ◽  
Alexandra Medvedeva ◽  
Marina N Rowen ◽  
...  
Keyword(s):  
Reproductive Tract ◽  
Implantation Rate ◽  
Conditional Knockout ◽  
Uterine Receptivity ◽  
Placental Development ◽  
Human Escs ◽  
Embryo Survival ◽  
Autophagy Gene ◽  
Decidual Cells

Abstract Uterine receptivity is critical for establishing and maintaining pregnancy. For the endometrium to become receptive, stromal cells must differentiate into decidual cells capable of secreting factors necessary for embryo survival and placental development. Although there are multiple reports of autophagy induction correlated with endometrial stromal cell (ESC) decidualization, the role of autophagy in decidualization has remained elusive. To determine the role of autophagy in decidualization, we utilized 2 genetic models carrying mutations to the autophagy gene Atg16L1. Although the hypomorphic Atg16L1 mouse was fertile and displayed proper decidualization, conditional knockout in the reproductive tract of female mice reduced fertility by decreasing the implantation rate. In the absence of Atg16L1, ESCs failed to properly decidualize and fewer blastocysts were able to implant. Additionally, small interfering RNA knock down of Atg16L1 was detrimental to the decidualization response of human ESCs. We conclude that Atg16L1 is necessary for decidualization, implantation, and overall fertility in mice. Furthermore, considering its requirement for human endometrial decidualization, these data suggest Atg16L1 may be a potential mediator of implantation success in women.

scholarly journals Function of Pumilio Genes in Human Embryonic Stem Cells and Their Effect in Stemness and Cardiomyogenesis

2019 ◽  
Author(s):  
Isabelle Leticia Zaboroski Silva ◽  
Anny Waloski Robert ◽  
Guillermo Cabrera Cabo ◽  
Lucia Spangenberg ◽  
Marco Augusto Stimamiglio ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Embryonic Stem ◽  
Mrna Levels ◽  
Human Escs ◽  
Mrna Targets ◽  
Cardiomyogenic Differentiation

AbstractPosttranscriptional regulation plays a fundamental role in the biology of embryonic stem cells (ESCs). Many studies have demonstrated that multiple mRNAs are coregulated by one or more RNA binding proteins (RBPs) that orchestrate the expression of these molecules. A family of RBPs, known as PUF (Pumilio-FBF), is highly conserved among species and has been associated with the undifferentiated and differentiated states of different cell lines. In humans, two homologs of the PUF family have been found: Pumilio 1 (PUM1) and Pumilio 2 (PUM2). To understand the role of these proteins in human ESCs (hESCs), we first demonstrated the influence of the silencing of PUM1 and PUM2 on pluripotency genes. OCT4 and NANOG mRNA levels decreased significantly with the knockdown of Pumilio, suggesting that PUMILIO proteins play a role in the maintenance of pluripotency in hESCs. Furthermore, we observed that the hESCs silenced for PUM1 and 2 exhibited an improvement in efficiency of in vitro cardiomyogenic differentiation. Using in silico analysis, we identified mRNA targets of PUM1 and PUM2 expressed during cardiomyogenesis. With the reduction of PUM1 and 2, these target mRNAs would be active and could be involved in the progression of cardiomyogenesis.

Metabolic Circuit Design Automation by Multi-objective BioCAD

2016 ◽  
pp. 30-44 ◽  
Author(s):  
Andrea Patané ◽  
Piero Conca ◽  
Giovanni Carapezza ◽  
Andrea Santoro ◽  
Jole Costanza ◽  
...  
Keyword(s):  
Circuit Design ◽  
Design Automation ◽  
Multi Objective ◽  
Metabolic Circuit

scholarly journals Transfer of Synthetic Human Chromosome into Human Induced Pluripotent Stem Cells for Biomedical Applications

Cells ◽  
2018 ◽  
Vol 7 (12) ◽  
pp. 261 ◽  
Author(s):  
Sergey Sinenko ◽  
Elena Skvortsova ◽  
Mikhail Liskovykh ◽  
Sergey Ponomartsev ◽  
Andrey Kuzmin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Biomedical Applications ◽  
Fluorescent Protein ◽  
Leukemia Virus ◽  
Embryonic Stem ◽  
Induced Pluripotent Stem Cell ◽  
Human Artificial Chromosome ◽  
Human Escs ◽  
Human Ipscs ◽  
Induced Pluripotent

AlphoidtetO-type human artificial chromosome (HAC) has been recently synthetized as a novel class of gene delivery vectors for induced pluripotent stem cell (iPSC)-based tissue replacement therapeutic approach. This HAC vector was designed to deliver copies of genes into patients with genetic diseases caused by the loss of a particular gene function. The alphoidtetO-HAC vector has been successfully transferred into murine embryonic stem cells (ESCs) and maintained stably as an independent chromosome during the proliferation and differentiation of these cells. Human ESCs and iPSCs have significant differences in culturing conditions and pluripotency state in comparison with the murine naïve-type ESCs and iPSCs. To date, transferring alphoidtetO-HAC vector into human iPSCs (hiPSCs) remains a challenging task. In this study, we performed the microcell-mediated chromosome transfer (MMCT) of alphoidtetO-HAC expressing the green fluorescent protein into newly generated hiPSCs. We used a recently modified MMCT method that employs an envelope protein of amphotropic murine leukemia virus as a targeting cell fusion agent. Our data provide evidence that a totally artificial vector, alphoidtetO-HAC, can be transferred and maintained in human iPSCs as an independent autonomous chromosome without affecting pluripotent properties of the cells. These data also open new perspectives for implementing alphoidtetO-HAC as a gene therapy tool in future biomedical applications.

scholarly journals Doxycycline supplementation allows for the culture of human ESCs/iPSCs with media changes at 3-day intervals

2015 ◽  
Vol 15 (3) ◽  
pp. 608-613 ◽  
Author(s):  
Mi-Yoon Chang ◽  
Boram Oh ◽  
Yong-Hee Rhee ◽  
Sang-Hun Lee
Keyword(s):  
Human Escs

Sequential Genetic Modification of the hprt Locus in Human ESCs Combining Gene Targeting and Recombinase-Mediated Cassette Exchange

2008 ◽  
Vol 10 (2) ◽  
pp. 217-230 ◽  
Author(s):  
Alexandra I. Di Domenico ◽  
Ioannis Christodoulou ◽  
Steve C. Pells ◽  
Jim McWhir ◽  
Alison J. Thomson
Keyword(s):  
Gene Targeting ◽  
Genetic Modification ◽  
Human Escs ◽  
Cassette Exchange ◽  
Hprt Locus

scholarly journals A Non-canonical BCOR-PRC1.1 Complex Represses Differentiation Programs in Human ESCs

2018 ◽  
Vol 22 (2) ◽  
pp. 235-251.e9 ◽  
Author(s):  
Zheng Wang ◽  
Micah D. Gearhart ◽  
Yu-Wei Lee ◽  
Ishan Kumar ◽  
Bulat Ramazanov ◽  
...  
Keyword(s):  
Human Escs
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