scholarly journals Myogenic Progenitor Cells Control Extracellular Matrix Production by Fibroblasts during Skeletal Muscle Hypertrophy

2017 ◽  
Vol 20 (1) ◽  
pp. 56-69 ◽  
Author(s):  
Christopher S. Fry ◽  
Tyler J. Kirby ◽  
Kate Kosmac ◽  
John J. McCarthy ◽  
Charlotte A. Peterson
Keyword(s):  
Skeletal Muscle ◽  
Extracellular Matrix ◽  
Progenitor Cells ◽  
Muscle Hypertrophy ◽  
Skeletal Muscle Hypertrophy ◽  
Extracellular Matrix Production ◽  
Matrix Production ◽  
Myogenic Progenitor Cells ◽  
Myogenic Progenitor

scholarly journals The Combination of Electroacupuncture and Massage Therapy Alleviates Myofibroblast Transdifferentiation and Extracellular Matrix Production in Blunt Trauma-Induced Skeletal Muscle Fibrosis

2021 ◽  
Vol 2021 ◽  
pp. 1-10
Author(s):  
Na Zhao ◽  
Bo Liu ◽  
Si-Wen Liu ◽  
Wei Zhang ◽  
Hua-Nan Li ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Extracellular Matrix ◽  
Growth Factor ◽  
Blunt Trauma ◽  
Massage Therapy ◽  
Muscle Fibrosis ◽  
Extracellular Matrix Production ◽  
Matrix Production ◽  
Skeletal Muscle Fibrosis ◽  
Tgf Β1

Complementary therapies, such as acupuncture and massage, had been previously reported to have therapeutic effects on skeletal muscle contusions. However, the recovery mechanisms on skeletal muscles after blunt trauma via the combination of electroacupuncture (EA) and massage therapy remain unclear. In the present study, a rat model of the skeletal muscle fibrosis following blunt trauma to rat skeletal muscle was established, and the potential molecular mechanisms of EA + massage therapy on the skeletal muscle fibrosis were investigated. The results suggested that EA + massage therapy could significantly decrease inflammatory cells infiltration and collagenous fiber content and ameliorate the disarrangement of sarcomeres within myofibrils compared to the model group. Further analysis revealed that EA + massage therapy could reduce the degree of fibrosis and increase the degree of myofibroblast apoptosis by downregulating the mRNA and protein expression of transforming growth factor- (TGF-) β1 and connective tissue growth factor (CTGF). Furthermore, the fibrosis of injured skeletal muscle was inhibited after treatment through the normalization of balance between matrix metalloproteinase- (MMP-) 1 and tissue inhibitor of matrix metalloproteinase (TIMP). These findings suggested that the combination of electroacupuncture and massage therapy could alleviate the fibrotic process by regulating TGF β1-CTGF-induced myofibroblast transdifferentiation and MMP-1/TIMP-1 balance for extracellular matrix production.

Rad is temporally regulated within myogenic progenitor cells during skeletal muscle regeneration

2006 ◽  
Vol 290 (2) ◽  
pp. C379-C387 ◽  
Author(s):  
Thomas J. Hawke ◽  
Shane B. Kanatous ◽  
Cindy M. Martin ◽  
Sean C. Goetsch ◽  
Daniel J. Garry
Keyword(s):  
Skeletal Muscle ◽  
Progenitor Cells ◽  
Cell Population ◽  
Muscle Regeneration ◽  
Progenitor Cell ◽  
Transcriptional Activity ◽  
Skeletal Muscle Regeneration ◽  
Progenitor Cell Population ◽  
Myogenic Progenitor Cells ◽  
Myogenic Progenitor

The successful use of myogenic progenitor cells for therapeutic applications requires an understanding of the intrinsic and extrinsic cues involved in their regulation. Herein we demonstrate the expression pattern and transcriptional regulation of Rad, a prototypical member of a family of novel Ras-related GTPases, during mammalian development and skeletal muscle regeneration. Rad was identified using microarray analysis, which revealed robust upregulation of its expression during skeletal muscle regeneration. Our current findings demonstrate negligible Rad expression with resting adult skeletal muscle; however, after muscle injury, Rad is expressed within the myogenic progenitor cell population. Rad expression is significantly increased and localized to the myogenic progenitor cell population during the early phases of regeneration and within the newly regenerated myofibers during the later phases of regeneration. Immunohistochemical analysis demonstrated that Rad and MyoD are coexpressed within the myogenic progenitor cell population of regenerating skeletal muscle. This expression profile of Rad during skeletal muscle regeneration is consistent with the proposed roles for Rad in the inhibition of L-type Ca2+channel activity and the inhibition of Rho/RhoA kinase activity. We also have demonstrated that known myogenic transcription factors (MEF2, MyoD, and Myf-5) can increase the transcriptional activity of the Rad promoter and that this ability is significantly enhanced by the presence of the Ca2+-dependent phosphatase calcineurin. Furthermore, this enhanced transcriptional activity appears to be dependent on the presence of a conserved NFAT binding motif within the Rad promoter. Taken together, these data define Rad as a novel factor within the myogenic progenitor cells of skeletal muscle and identify key regulators of its transcriptional activity.

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