scholarly journals Targeted Application of Human Genetic Variation Can Improve Red Blood Cell Production from Stem Cells

2016 ◽  
Vol 18 (1) ◽  
pp. 73-78 ◽  
Author(s):  
Felix C. Giani ◽  
Claudia Fiorini ◽  
Aoi Wakabayashi ◽  
Leif S. Ludwig ◽  
Rany M. Salem ◽  
...  
Keyword(s):  
Stem Cells ◽  
Genetic Variation ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Human Genetic Variation ◽  
Cell Production

Differentiation of Human Pluripotent Stem Cells for Red Blood Cell Production

2018 ◽  
pp. 47-62
Author(s):  
Mark C. Allenby ◽  
Susana Brito dos Santos ◽  
Nicki Panoskaltsis ◽  
Athanasios Mantalaris
Keyword(s):  
Stem Cells ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Pluripotent Stem Cells ◽  
Human Pluripotent Stem Cells ◽  
Cell Production

scholarly journals Controlling the production of blood cells

Blood ◽  
1979 ◽  
Vol 53 (1) ◽  
pp. 157-165 ◽  
Author(s):  
MJ Cline ◽  
DW Golde
Keyword(s):  
Stem Cells ◽  
Blood Cell ◽  
Blood Cells ◽  
Cellular Interactions ◽  
Cell Production

Abstract The development since 1966 of a technology for growing stem cells in vitro has provided new insights into the controls of blood cell production. Hematopoietic hormones have been purified and important cellular interactions in hematopoiesis have been defined.

GENETIC VARIATION OF RED BLOOD CELL ENZYMES IN THE DOG

1974 ◽  
Vol 17 (1) ◽  
pp. 115-120 ◽  
Author(s):  
P. WEIDEN ◽  
R. STORB ◽  
H. J. KOLB ◽  
T. GRAHAM ◽  
J. ANDERSON ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Blood Cell ◽  
Red Blood Cell

Lipocalin 2 functions as a negative regulator of red blood cell production in an autocrine fashion

2005 ◽  
Vol 19 (13) ◽  
pp. 1881-1883 ◽  
Author(s):  
Ken‐Ichi Miharada ◽  
Takashi Hiroyama ◽  
Kazuhiro Sudo ◽  
Toshiro Nagasawa ◽  
Yukio Nakamura
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Negative Regulator ◽  
Lipocalin 2 ◽  
Cell Production

Red blood cell generation from human induced pluripotent stem cells of different donor cell type origin

2013 ◽  
Vol 41 (8) ◽  
pp. S27
Author(s):  
Isabel Dorn ◽  
Katharina Klich ◽  
Martina Radstaak ◽  
Katherina Psathaki ◽  
Marcos Arauzo-Bravo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Blood Cell ◽  
Induced Pluripotent Stem Cells ◽  
Red Blood Cell ◽  
Pluripotent Stem Cells ◽  
Donor Cell ◽  
Cell Generation ◽  
Cell Type ◽  
Induced Pluripotent ◽  
Donor Cell Type

New Role of the Regulatory Gene SOX2 in Hematopoiesis.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 4195-4195
Author(s):  
Elena Levantini ◽  
Francesca Bertolotti ◽  
Francesco Cerisoli ◽  
Anna L. Ferri ◽  
Elisa Brescia ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Blood Cell ◽  
Molecular Mechanisms ◽  
Bone Marrow Cells ◽  
Regulatory Gene ◽  
Embryonic Stem ◽  
Mutant Mice ◽  
Wild Type ◽  
Cell Production

Abstract Several genes encoding transcription factors of different families have been implicated in the development and differentiation of multiple cell systems. The Sry-type high-mobility-group box 2 gene (Sox2) encodes a transcription factor that is expressed in very early cells such as embryonic stem cells and neural stem cells, where it plays important functional roles (Genes and Dev.17:126, 2003; Development131:3805, 2004). To investigate whether Sox2 plays a role also in blood cell production, we first analyzed its expression in murine hematopoietic cells. Results indicate that the gene is transcriptionally active at low levels in primitive progenitors. Furthermore, in order to address the functional implication of Sox2 in hematopoiesis we analyzed mature and precursor cells in mutant mice compound heterozygotes for a null Sox2 allele and for the deletion of a Sox2 5′ enhancer, as the complete inactivation of the gene in homozygosis is embryonic lethal. At the peripheral blood level we did not detect significant variations in the mutants. However analysis of bone marrow precursors in clonogenic assays showed that Sox2 knock-down mice exhibited a significant increase in the number of multipotent precursors, as compared to wild type animals. Moreover, bone marrow cells of wild type and mutant mice were analyzed for the expression of a panel of regulatory genes involved in the control of different somatic stem cells. Preliminary evidence suggests that some of these genes are modulated in the mutant cells. These observations support the view that Sox2 plays a role at early stages of blood cell production, providing further evidence that common molecular mechanisms may be involved in the regulation of several different types of multipotent cells.

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