scholarly journals The THO Complex Regulates Pluripotency Gene mRNA Export and Controls Embryonic Stem Cell Self-Renewal and Somatic Cell Reprogramming

2013 ◽  
Vol 13 (6) ◽  
pp. 676-690 ◽  
Author(s):  
Li Wang ◽  
Yi-Liang Miao ◽  
Xiaofeng Zheng ◽  
Brad Lackford ◽  
Bingying Zhou ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Somatic Cell ◽  
Mrna Export ◽  
Embryonic Stem ◽  
Cell Reprogramming ◽  
Self Renewal ◽  
Somatic Cell Reprogramming ◽  
Pluripotency Gene ◽  
Gene Mrna

scholarly journals E‐cadherin is crucial for embryonic stem cell pluripotency and can replace OCT4 during somatic cell reprogramming

EMBO Reports ◽  
2011 ◽  
Vol 12 (7) ◽  
pp. 720-726 ◽  
Author(s):  
Torben Redmer ◽  
Sebastian Diecke ◽  
Tamara Grigoryan ◽  
Angel Quiroga‐Negreira ◽  
Walter Birchmeier ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Somatic Cell ◽  
Embryonic Stem ◽  
Cell Reprogramming ◽  
Somatic Cell Reprogramming ◽  
Stem Cell Pluripotency ◽  
Embryonic Stem Cell Pluripotency ◽  
E Cadherin

scholarly journals INO80 Facilitates Pluripotency Gene Activation in Embryonic Stem Cell Self-Renewal, Reprogramming, and Blastocyst Development

2014 ◽  
Vol 14 (5) ◽  
pp. 575-591 ◽  
Author(s):  
Li Wang ◽  
Ying Du ◽  
James M. Ward ◽  
Takashi Shimbo ◽  
Brad Lackford ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Gene Activation ◽  
Embryonic Stem ◽  
Blastocyst Development ◽  
Self Renewal ◽  
Pluripotency Gene

scholarly journals Impaired stem cell differentiation and somatic cell reprogramming in DIDO3 mutants with altered RNA processing and increased R-loop levels

2021 ◽  
Vol 12 (7) ◽  
Author(s):  
Agnes Fütterer ◽  
Amaia Talavera-Gutiérrez ◽  
Tirso Pons ◽  
Jesús de Celis ◽  
Julio Gutiérrez ◽  
...  
Keyword(s):  
Stem Cell ◽  
Somatic Cell ◽  
Rna Processing ◽  
Transcription Termination ◽  
Regulation Of Gene Expression ◽  
Embryonic Stem ◽  
Stem Cell Differentiation ◽  
Cell Reprogramming ◽  
Main Role ◽  
Somatic Cell Reprogramming

AbstractEmbryonic stem cell (ESC) differentiation and somatic cell reprogramming are biological processes governed by antagonistic expression or repression of a largely common set of genes. Accurate regulation of gene expression is thus essential for both processes, and alterations in RNA processing are predicted to negatively affect both. We show that truncation of the DIDO gene alters RNA splicing and transcription termination in ESC and mouse embryo fibroblasts (MEF), which affects genes involved in both differentiation and reprogramming. We combined transcriptomic, protein interaction, and cellular studies to identify the underlying molecular mechanism. We found that DIDO3 interacts with the helicase DHX9, which is involved in R-loop processing and transcription termination, and that DIDO3-exon16 deletion increases nuclear R-loop content and causes DNA replication stress. Overall, these defects result in failure of ESC to differentiate and of MEF to be reprogrammed. MEF immortalization restored impaired reprogramming capacity. We conclude that DIDO3 has essential functions in ESC differentiation and somatic cell reprogramming by supporting accurate RNA metabolism, with its exon16-encoded domain playing the main role.

Sign in / Sign up

Export Citation Format

Share Document