scholarly journals Directed Differentiation of Human Embryonic Stem Cells into Functional Retinal Pigment Epithelium Cells

2009 ◽  
Vol 5 (4) ◽  
pp. 396-408 ◽  
Author(s):  
Maria Idelson ◽  
Ruslana Alper ◽  
Alexey Obolensky ◽  
Etti Ben-Shushan ◽  
Itzhak Hemo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Retinal Pigment Epithelium ◽  
Human Embryonic Stem Cells ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Embryonic Stem ◽  
Directed Differentiation ◽  
Retinal Pigment Epithelium Cells ◽  
Epithelium Cells

scholarly journals Investigation of Differentiated Embryonic Stem Cells Growth on Optimized Porous Polymeric Bed with Fuzzy System

2021 ◽  
Author(s):  
Saleheh Shahmoradi ◽  
Fatemeh Yazdian ◽  
Amin Janghorbani ◽  
Leila Satarian ◽  
Farnaz Behroozi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Embryonic Stem ◽  
Age Related Macular Degeneration ◽  
Age Related ◽  
Retinal Pigment Epithelium Cells ◽  
Epithelium Cells

Introduction: Age-related macular degeneration (AMD) is one of the retina diseases in which retinal pigment epithelium cells are degraded and lead to blindness. Available treatments only slow down the progression of it. In this study, human embryonic stem cells (hESCs) differentiated into retinal pigment epithelium cells were cultured on a polycaprolactone scaffold. Methods: The optimization of the diameter of the produced scaffolds by electrospinning method was done using the fuzzy method for the first time. To improve cell adhesion and proliferation, related parameters to alkaline hydrolysis method were optimized and hydrophobic surface of scaffold was modified. After in vitro analysis, cells were cultured on different groups of scaffolds. In vivo analyses were done and cells culture on scaffolds observed. Results: The optimal parameters for the scaffold based on the fuzzy model were 18.1 kV for voltage, 0.07 g / ml for solution concentration and 115 nm for scaffold diameter, respectively. The immersion time of the scaffold in alkaline solution and concentration of solution were measured 97 min and 3.7 M, respectively. The treated scaffold had a higher degradation rate and water adsorption. MTT-Assay results showed that scaffolds with modified surfaces had a higher amount of cell viability and proliferation after 7 days. SEM image results confirmed this finding after almost two months. Additionally, the results of ICC test showed that after passing this time, cells kept their RPE and epithelium. Conclusion: Based on the results, the hydrolyzed scaffold is a suitable substrate for cell proliferation and can be a good option for AMD treatment.

Semi-automatic Method for Ca2+ Imaging Data Analysis of Maturing Human Embryonic Stem Cells-Derived Retinal Pigment Epithelium

2016 ◽  
Vol 44 (11) ◽  
pp. 3408-3420 ◽  
Author(s):  
Amna E. Abu Khamidakh ◽  
Florentino Caetano dos Santos ◽  
Heli Skottman ◽  
Kati Juuti-Uusitalo ◽  
Jari Hyttinen
Keyword(s):  
Stem Cells ◽  
Data Analysis ◽  
Embryonic Stem Cells ◽  
Retinal Pigment Epithelium ◽  
Human Embryonic Stem Cells ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Embryonic Stem ◽  
Automatic Method ◽  
Imaging Data

scholarly journals Derivation and Comparative Assessment of Retinal Pigment Epithelium from Human Embryonic Stem Cells Using Transcriptomics

2004 ◽  
Vol 6 (3) ◽  
pp. 217-245 ◽  
Author(s):  
Irina Klimanskaya ◽  
Jason Hipp ◽  
Kourous A. Rezai ◽  
Michael West ◽  
Anthony Atala ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Retinal Pigment Epithelium ◽  
Human Embryonic Stem Cells ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Embryonic Stem ◽  
Comparative Assessment
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