scholarly journals Histone Deacetylase Inhibition Elicits an Evolutionarily Conserved Self-Renewal Program in Embryonic Stem Cells

2009 ◽  
Vol 4 (4) ◽  
pp. 359-369 ◽  
Author(s):  
Carol B. Ware ◽  
Linlin Wang ◽  
Brigham H. Mecham ◽  
Lanlan Shen ◽  
Angelique M. Nelson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Histone Deacetylase ◽  
Embryonic Stem ◽  
Self Renewal ◽  
Histone Deacetylase Inhibition ◽  
Renewal Program ◽  
Evolutionarily Conserved

scholarly journals Lysines Acetylome and Methylome Profiling of H3 and H4 Histones in Trichostatin A—Treated Stem Cells

2021 ◽  
Vol 22 (4) ◽  
pp. 2063
Author(s):  
Flora Cozzolino ◽  
Ilaria Iacobucci ◽  
Vittoria Monaco ◽  
Tiziana Angrisano ◽  
Maria Monti
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Histone Deacetylase ◽  
Trichostatin A ◽  
Limited Proteolysis ◽  
Embryonic Stem ◽  
Epigenetic Modifications ◽  
Lysine Methylation ◽  
Histone Deacetylase Inhibition ◽  
Lysine Residues

Trichostatin A ([R-(E,E)]-7-[4-(dimethylamino) phenyl]-N-hydroxy- 4,6-dimethyl- 7-oxo-2,4-heptadienamide, TSA) affects chromatin state through its potent histone deacetylase inhibitory activity. Interfering with the removal of acetyl groups from lysine residues in histones is one of many epigenetic regulatory processes that control gene expression. Histone deacetylase inhibition drives cells toward the differentiation stage, favoring the activation of specific genes. In this paper, we investigated the effects of TSA on H3 and H4 lysine acetylome and methylome profiling in mice embryonic stem cells (ES14), treated with trichostatin A (TSA) by using a new, untargeted approach, consisting of trypsin-limited proteolysis experiments coupled with MALDI-MS and LC-MS/MS analyses. The method was firstly set up on standard chicken core histones to probe the optimized conditions in terms of enzyme:substrate (E:S) ratio and time of proteolysis and, then, applied to investigate the global variations of the acetylation and methylation state of lysine residues of H3 and H4 histone in the embryonic stem cells (ES14) stimulated by TSA and addressed to differentiation. The proposed strategy was found in its simplicity to be extremely effective in achieving the identification and relative quantification of some of the most significant epigenetic modifications, such as acetylation and lysine methylation. Therefore, we believe that it can be used with equal success in wider studies concerning the characterization of all epigenetic modifications.

scholarly journals Effects of Ionizing Radiation on Self-Renewal and Pluripotency of Human Embryonic Stem Cells

2010 ◽  
Vol 70 (13) ◽  
pp. 5539-5548 ◽  
Author(s):  
Kitchener D. Wilson ◽  
Ning Sun ◽  
Mei Huang ◽  
Wendy Y. Zhang ◽  
Andrew S. Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Ionizing Radiation ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Self Renewal

scholarly journals Musashi2 Is Required for the Self-Renewal and Pluripotency of Embryonic Stem Cells

PLoS ONE ◽  
2012 ◽  
Vol 7 (4) ◽  
pp. e34827 ◽  
Author(s):  
Erin L. Wuebben ◽  
Sunil K. Mallanna ◽  
Jesse L. Cox ◽  
Angie Rizzino
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
The Self ◽  
Self Renewal

scholarly journals Fam60a defines a variant Sin3a‐Hdac complex in embryonic stem cells required for self‐renewal

2017 ◽  
Vol 36 (15) ◽  
pp. 2216-2232 ◽  
Author(s):  
Gundula Streubel ◽  
Darren J Fitzpatrick ◽  
Giorgio Oliviero ◽  
Andrea Scelfo ◽  
Bruce Moran ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Self Renewal

scholarly journals Environmental Oxygen Tension Regulates the Energy Metabolism and Self-Renewal of Human Embryonic Stem Cells

PLoS ONE ◽  
2013 ◽  
Vol 8 (5) ◽  
pp. e62507 ◽  
Author(s):  
Catherine E. Forristal ◽  
David R. Christensen ◽  
Fay E. Chinnery ◽  
Raffaella Petruzzelli ◽  
Kate L. Parry ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Energy Metabolism ◽  
Oxygen Tension ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Self Renewal

scholarly journals RNA Polymerase II-Association Factor 1 (Paf1/PD2) Maintains Self-renewal by Its Interaction with Oct3/4 in Mouse Embryonic Stem Cells

Stem Cells ◽  
2009 ◽  
pp. N/A-N/A ◽  
Author(s):  
Moorthy P. Ponnusamy ◽  
Shonali Deb ◽  
Parama Dey ◽  
Subhankar Chakraborty ◽  
Satyanarayana Rachagani ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Rna Polymerase ◽  
Rna Polymerase Ii ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Self Renewal

scholarly journals Comprehensive Identification of Krüppel-Like Factor Family Members Contributing to the Self-Renewal of Mouse Embryonic Stem Cells and Cellular Reprogramming

PLoS ONE ◽  
2016 ◽  
Vol 11 (3) ◽  
pp. e0150715 ◽  
Author(s):  
Hyojung Jeon ◽  
Tsuyoshi Waku ◽  
Takuya Azami ◽  
Le Tran Phuc Khoa ◽  
Jun Yanagisawa ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Family Members ◽  
Embryonic Stem ◽  
Mouse Embryonic Stem Cells ◽  
Cellular Reprogramming ◽  
The Self ◽  
Self Renewal
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