scholarly journals Genetic Modification of Human Embryonic Stem Cells for Derivation of Target Cells

2008 ◽  
Vol 2 (5) ◽  
pp. 422-433 ◽  
Author(s):  
Antonietta Giudice ◽  
Alan Trounson
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Genetic Modification ◽  
Embryonic Stem ◽  
Target Cells

Generation of transducible versions of transcription factors Oct4 and Sox2

2008 ◽  
Vol 389 (7) ◽  
Author(s):  
Manal Bosnali ◽  
Frank Edenhofer
Keyword(s):  
Stem Cells ◽  
Transcription Factors ◽  
Embryonic Stem Cells ◽  
Genetic Modification ◽  
Fusion Proteins ◽  
Embryonic Stem ◽  
Vector System ◽  
Target Cells ◽  
Protein Transduction ◽  
Cellular Delivery

Abstract The transcription factors Oct4 and Sox2 are two of the main regulators of pluripotency in embryonic stem cells. Since the importance of non-genetic modification is continually increasing, particularly for therapeutic application of manipulated cells, the aim of the present study was to generate cell-permeant Oct4 and Sox2 proteins for the direct cellular delivery of active proteins. Protein transduction allowing cellular manipulation to circumvent genetic modification of target cells has recently been developed. We present a new expression vector system, pSESAME, that facilitates the generation of transducible proteins. Using pSESAME, both Oct4 and Sox2 were genetically fused with a TAT protein transduction domain that promotes cellular penetration. The recombinant purified Oct4 and Sox2 fusion proteins display DNA-binding properties comparable to their endogenous counterparts, and exhibit cellular entry and the ability to modulate the transcriptional machinery maintaining pluripotency of mouse embryonic stem cells. In a rescue assay we demonstrate that transducible Oct4 and Sox2 fusion proteins can compensate knockdown of Pou5f1 and Sox2, respectively. This study provides powerful tools for the modulation of stem cell properties without genetic interference.

A method for genetic modification of human embryonic stem cells using electroporation

2007 ◽  
Vol 2 (4) ◽  
pp. 792-796 ◽  
Author(s):  
Magdaline Costa ◽  
Mirella Dottori ◽  
Koula Sourris ◽  
Pegah Jamshidi ◽  
Tanya Hatzistavrou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Genetic Modification ◽  
Embryonic Stem

scholarly journals Stable genetic modification of human embryonic stem cells by lentiviral vectors

2003 ◽  
Vol 7 (2) ◽  
pp. 281-287 ◽  
Author(s):  
Michal Gropp ◽  
Pavel Itsykson ◽  
Orna Singer ◽  
Tamir Ben-Hur ◽  
Etti Reinhartz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Genetic Modification ◽  
Embryonic Stem ◽  
Lentiviral Vectors

Feeder-free culture of human embryonic stem cells in conditioned medium for efficient genetic modification

2008 ◽  
Vol 3 (9) ◽  
pp. 1435-1443 ◽  
Author(s):  
Stefan R Braam ◽  
Chris Denning ◽  
Elena Matsa ◽  
Lorraine E Young ◽  
Robert Passier ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Conditioned Medium ◽  
Human Embryonic Stem Cells ◽  
Genetic Modification ◽  
Embryonic Stem
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