One-pot and one-step colorimetric detection of aminopeptidase N activity based on gold nanoparticles-based supramolecular structure

2018 ◽  
Vol 267 ◽  
pp. 336-341 ◽  
Author(s):  
Xiaoxia Mao ◽  
Yifei Li ◽  
Peng Han ◽  
Xiaohong Wang ◽  
Shiqi Yang ◽  
...  
2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Sidhureddy Boopathi ◽  
Shanmugam Senthilkumar ◽  
Kanala Lakshminarasimha Phani

In this work, we reported for the first time, a facile and one step synthesis of gold nanoparticles from HAuCl4, employing tetraphenylborate as the reducing agent. The synthesis is not only facile but also yields “dumb-bell-shaped”particles. This shape appears to arise from a possible emulsion of the products of oxidation/decomposition of tetraphenylborate by HAuCl4, surrounding the particle. The size and shape of the AuNPs were characterized by Transmission electron microscopy (TEM) and UV-visible Spectroscopy. Interestingly, the addition of polyvinylpyrrolidone (PVP) during the synthesis was found to enhance the stability of the nanoparticle dispersion. The particles synthesized under these conditions assume “spherical” shape with the appearance of only transverse surface plasmon resonance band. The highlight of the observations is that the gold nanoparticles synthesized using tetraphenylborate as reducing agent and PVP as stabilizer are highly stable in alkaline medium, in contrast to the synthesis wherein borohydride is used as reducing agent. The AuNPs synthesized using tetraphenylborate and PVP show their mercury sensing behavior only in the alkaline medium. The color of the nanoparticle dispersion undergoes distinct color change from pink to blue with the addition of mercury ions. They also show dramatic selectivity to mercury ions in presence of other interfering ions, Pb2+, Zn2+and Ca2+.


RSC Advances ◽  
2015 ◽  
Vol 5 (15) ◽  
pp. 11458-11468 ◽  
Author(s):  
M. Annadhasan ◽  
J. Kasthuri ◽  
N. Rajendiran

A novel, green, one-pot and energy efficient route has been developed for the synthesis of gold nanoparticles by natural sunlight irradiation, and they were utilized effectively for the colorimetric detection of Ni2+ and Co2+ ions.


2021 ◽  
Vol 8 (5) ◽  
pp. 210185
Author(s):  
Daim Asif Raja ◽  
Fazeelah Munir ◽  
Muhammad Raza Shah ◽  
Muhammad Iqbal Bhanger ◽  
Muhammad Imran Malik

The development of metal nanoparticle-based facile colorimetric assays for drugs and insecticides is an emerging area of current scientific research. In the present work, polypropylene glycol was used for stabilization of gold nanoparticles (AuNPs) in a simple one-pot two-phase process and subsequently employed it for the specific detection of cephradine (CPH). The characterization of the prepared PPG-AuNPs was conducted through various analytical techniques such as UV-visible spectrophotometry, Fourier transform infrared spectroscopy, atomic force microscopy (AFM), zeta potential and zetasizer techniques. As the major target of the study, the stabilized PPG-AuNPs were employed for colorimetric detection of CPH and other drugs. Typical wine-red colour of PPG-AuNPs disappeared immediately and surface plasmon resonance band quenched by addition of CPH in the presence of several other interferents (drugs and salts) and in real samples. PPG-AuNPs permitted efficient, selective, reliable and rapid determination in a concentration range of 0.01–120 mM with a detection limit (LoD) of 11.0 mM. The developed sensor has the potential to be used for fast scanning of pharmaceutical formulations for quantification of CPH at production facilities.


2020 ◽  
Vol 6 (5) ◽  
pp. 2805-2811 ◽  
Author(s):  
Ying Zhou ◽  
Congdi Li ◽  
Rong Liu ◽  
Zenglong Chen ◽  
Li Li ◽  
...  

2017 ◽  
Vol 9 (19) ◽  
pp. 2805-2811 ◽  
Author(s):  
Jianyu Wei ◽  
Luming Yang ◽  
Guozong Yue ◽  
Jing Zhu ◽  
Deshun Huang ◽  
...  

Nitrilotriacetate (NTA) has been reported to behave as both a reductant and a stabilizer for gold nanoparticle (AuNP) preparation in a one-pot synthesis and colorimetric assay.


2021 ◽  
Vol 5 (1) ◽  
pp. 6
Author(s):  
Nadezhda S. Komova ◽  
Ksenya V. Serebrennikova ◽  
Anna N. Berlina ◽  
Svetlana M. Pridvorova ◽  
Anatoly V. Zherdev ◽  
...  

The application of mercaptosuccinic acid-capped gold nanoparticles as a sensing probe for the colorimetric detection of Fe(III) is reported. The well-dispersed gold nanoparticles (AuNPs) with a diameter of around 20 nm were obtained by a one-step reaction of tetrachloroauratic acid with mercaptosuccinic acid (MSA) as a reducing and capping agent, respectively. Fe(III) reportedly causes the aggregation of prepared MSA-capped AuNPs followed by a change in color and a shift to long wavelengths in the absorbance spectra. The resulting method allows for a visual and spectrophotometric Fe(III) determination with detection limits of 30 ng/mL and 23 ng/mL, respectively. MSA-capped AuNPs have been used as sensing probes for the detection of Fe(III) in drinking water samples with a detection limit that is much lower than the maximum permissible level of Fe(III) specified by official regulations (300 ng/mL).


2018 ◽  
Vol 265 ◽  
pp. 285-292 ◽  
Author(s):  
Juan Du ◽  
Hina Singh ◽  
Wei-jie Dong ◽  
Yan-hong Bai ◽  
Tae-Hoo Yi

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