A molybdenum disulfide/gold nanorod composite-based electrochemical immunosensor for sensitive and quantitative detection of microcystin-LR in environmental samples

2017 ◽  
Vol 244 ◽  
pp. 606-615 ◽  
Author(s):  
Yanli Zhang ◽  
Meng Chen ◽  
Haiyan Li ◽  
Fuqing Yan ◽  
Pengfei Pang ◽  
...  
Keyword(s):  
Molybdenum Disulfide ◽  
Environmental Samples ◽  
Electrochemical Immunosensor ◽  
Gold Nanorod ◽  
Quantitative Detection

Layer-by-layer self-assembly of 2D graphene nanosheets, 3D copper oxide nanoflowers and 0D gold nanoparticles for ultrasensitive electrochemical detection of alpha fetoprotein

RSC Advances ◽  
2015 ◽  
Vol 5 (70) ◽  
pp. 56583-56589 ◽  
Author(s):  
Yulan Wang ◽  
Dan Wu ◽  
Yong Zhang ◽  
Xiang Ren ◽  
Yaoguang Wang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Copper Oxide ◽  
Electrochemical Detection ◽  
Self Assembly ◽  
Graphene Nanosheets ◽  
Electrochemical Immunosensor ◽  
Alpha Fetoprotein ◽  
Quantitative Detection ◽  
Layer By Layer ◽  
Label Free

In this work, a novel and ultrasensitive label-free electrochemical immunosensor was developed for the quantitative detection of alpha fetoprotein (AFP).

A sensitive and regenerative electrochemical immunosensor for quantitative detection of Escherichia coli O157:H7 based on stable polyaniline coated screen-printed carbon electrode and rGO-NR-Au@Pt

2019 ◽  
Vol 11 (11) ◽  
pp. 1475-1482 ◽  
Author(s):  
Xiaoyan Mo ◽  
Zunyi Wu ◽  
Jianfeng Huang ◽  
Guangying Zhao ◽  
Wenchao Dou
Keyword(s):  
Escherichia Coli ◽  
Electrochemical Immunosensor ◽  
Quantitative Detection ◽  
Carbon Electrode ◽  
Escherichia Coli O157 ◽  
E Coli ◽  
Screen Printed Carbon Electrode ◽  
Screen Printed

An electrochemical immunosensor was constructed for the detection of E. coli O157:H7 using Au@Pt, rGO and regenerative leucoemeraldine PANI/AuNPs.

scholarly journals Comparison of culture-based, vital stain and PMA-qPCR methods for the quantitative detection of viable hookworm ova

2017 ◽  
Vol 75 (11) ◽  
pp. 2615-2621 ◽  
Author(s):  
P. Gyawali ◽  
J. P. S. Sidhu ◽  
W. Ahmed ◽  
P. Jagals ◽  
S. Toze
Keyword(s):  
Environmental Samples ◽  
Quantitative Measurement ◽  
Quantitative Polymerase Chain Reaction ◽  
Detection Methods ◽  
Quantitative Detection ◽  
Propidium Monoazide ◽  
Chain Reaction ◽  
Vital Stain ◽  
Polymerase Chain ◽  
Hookworm Ova

Accurate quantitative measurement of viable hookworm ova from environmental samples is the key to controlling hookworm re-infections in the endemic regions. In this study, the accuracy of three quantitative detection methods [culture-based, vital stain and propidium monoazide-quantitative polymerase chain reaction (PMA-qPCR)] was evaluated by enumerating 1,000 ± 50 Ancylostoma caninum ova in the laboratory. The culture-based method was able to quantify an average of 397 ± 59 viable hookworm ova. Similarly, vital stain and PMA-qPCR methods quantified 644 ± 87 and 587 ± 91 viable ova, respectively. The numbers of viable ova estimated by the culture-based method were significantly (P < 0.05) lower than vital stain and PMA-qPCR methods. Therefore, both PMA-qPCR and vital stain methods appear to be suitable for the quantitative detection of viable hookworm ova. However, PMA-qPCR would be preferable over the vital stain method in scenarios where ova speciation is needed.

Nonenzymatic Electrochemical Immunosensor Using Ferroferric Oxide–Manganese Dioxide–Reduced Graphene Oxide Nanocomposite as Label for α-Fetoprotein Detection

NANO ◽  
2016 ◽  
Vol 11 (10) ◽  
pp. 1650116 ◽  
Author(s):  
Wensi Jian ◽  
Chunping Wang ◽  
Zuanguang Chen ◽  
Yanyan Yu ◽  
Duanping Sun ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Manganese Dioxide ◽  
Reduced Graphene Oxide ◽  
Photoelectron Spectroscopy ◽  
Low Cost ◽  
Synergetic Effect ◽  
Electrochemical Immunosensor ◽  
Quantitative Detection ◽  
Ferroferric Oxide ◽  
Reduced Graphene

A novel nonenzymatic electrochemical immunosensor was fabricated for quantitative detection of [Formula: see text]-fetoprotein (AFP). The immunosensor was constructed by modifying gold electrode with electrochemical reduction of graphene oxide-carboxyl multi-walled carbon nanotube composites (ERGO–CMWCNTs) and electrodeposition of gold nanoparticles (AuNPs) for effective immobilization of primary antibody (Ab[Formula: see text]. Ferroferric oxide–manganese dioxide–reduced graphene oxide nanocomposites (Fe3O4@MnO2–rGO) were designed as labels for signal amplification. On one hand, the excellent electroconductivity and outstanding electron transfer capability of ERGO–CMWCNTs/AuNPs improved the sensitivity of the immunosensor. On the other hand, introduction of rGO could not only increase the specific surface area for immobilization of secondary antibody (Ab[Formula: see text] but also build a synergetic effect to reinforce the electrocatalytic properties of catalysts. Fe3O4@MnO2–rGO nanocomposites were characterized by scanning electron microscope, Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. Using AFP as a model analyte, the proposed sandwich-type electrochemical immunosensor exhibited a wide linear range of 0.01–50[Formula: see text][Formula: see text] with a low detection limit of 5.8[Formula: see text][Formula: see text]. Moreover, the Fe3O4@MnO2–rGO-based peroxidase mimetic system displayed an excellent analytical performance with low cost, satisfactory reproducibility and high selectivity, which could be further extended for detecting other disease-related biomarkers.

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