scholarly journals A novel thiophene functionalized silicon-cored compounds: Aggregation-induced emission enhancement and aqueous fluorogenic Fe3+ probes in bovine serum albumin

2017 ◽  
Vol 241 ◽  
pp. 65-72 ◽  
Author(s):  
Xuefeng Wang ◽  
Hua Wang ◽  
Shengyu Feng
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Aggregation Induced Emission ◽  
Emission Enhancement

Aggregation-induced emission enhancement of anthracene-derived Schiff base compounds and their application as a sensor for bovine serum albumin and optical cell imaging

Luminescence ◽  
2018 ◽  
Vol 33 (4) ◽  
pp. 780-789 ◽  
Author(s):  
Simon Densil ◽  
Chien-Huei Chang ◽  
Chia-Ling Chen ◽  
Alagarsamy Mathavan ◽  
Arumugam Ramdass ◽  
...  
Keyword(s):  
Schiff Base ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Cell Imaging ◽  
Optical Cell ◽  
Aggregation Induced Emission ◽  
Emission Enhancement

Aggregation-Induced Emission Probes for Specific Turn-On Quantification of Bovine Serum Albumin

2020 ◽  
Vol 3 (8) ◽  
pp. 5193-5201
Author(s):  
Yuan Yu ◽  
Qing-Tian Gong ◽  
Wei-Fan Lu ◽  
Yan-Hong Liu ◽  
Zeng-Jie Yang ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Aggregation Induced Emission ◽  
Turn On

A multistimuli responsive heteroleptic iridium(iii) complex: role of hydrogen bonding in probing solvent, pH and bovine serum albumin (BSA)

2020 ◽  
Vol 8 (19) ◽  
pp. 6605-6614
Author(s):  
Vishal Kachwal ◽  
Parva Kumar Sharma ◽  
Amrit Sarmah ◽  
Shibasish Chowdhury ◽  
Inamur Rahaman Laskar
Keyword(s):  
Hydrogen Bonding ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Solvent Effect ◽  
Bovine Serum ◽  
Vital Role ◽  
Aggregation Induced Emission

This article focuses on the vital role of hydrogen bonding to explain unusual photophysical behaviours (sensing BSA, pH probing, specific solvent effect) of an ‘Aggregation-induced Emission’ (AIE) active Ir(iii) complex.

scholarly journals Potassium-Induced Emission Enhancement of Bovine Serum Albumin-Stabilized Red-Emitting Au Nanoclusters: Mechanism and Application to Blood Plasma

2021 ◽  
Author(s):  
Achmad Hidayat ◽  
Nicole Wawrzyniak ◽  
Adam Domanski ◽  
Yutaka Shibata ◽  
Akiko Hori ◽  
...  
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Blood Plasma ◽  
Emission Intensity ◽  
Bovine Serum ◽  
Gold Nanoclusters ◽  
Normal Function ◽  
Quantum Yields ◽  
Physiological Concentration ◽  
Emission Enhancement

Abstract Aggregation-induced emission (AIE) enhancement is attractive for bioimaging as it offers higher quantum yields (QYs) from fluorophores via modulation of their immediate environment. Fluorophores with high QYs are essential probes for investigating the spatiotemporal distribution of physiologically important metal ions such sodium and potassium. Potassium ions are vital for normal function of living organisms. The research reported here evaluates the emission intensity of the bovine serum albumin-stabilized red-emitting gold nanoclusters (BSA-rAuNCs) upon uptake of K+. The integrated sphere method was used to determine the absolute QYs and a custom-built fluorescence setup recorded the emission spectra of BSA-rAuNCs. Enhancement of emission intensity was observed upon increasing K+ concentration, within the physiological concentration range of 0 - 150 mM. The emission enhancement was correlated with the particle size and charge analysis. Aggregation of BSA-rAuNCs was found to be responsible for the observed emission enhancement. The QY of BSA-rAuNCs in bovine blood plasma was found to be four times lower than corresponding QY in water.

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

Stability of Prostacyclin in Human Plasma and Whole Blood: Studies on the Protective Effect of Albumin

1981 ◽  
Vol 46 (03) ◽  
pp. 645-647 ◽  
Author(s):  
M A Orchard ◽  
C Robinson
Keyword(s):  
Platelet Aggregation ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Protective Effect ◽  
Whole Blood ◽  
Bovine Serum ◽  
Fatty Acid Content ◽  
Krebs Solution ◽  
Antiaggregatory Activity ◽  
Free Plasma

SummaryThe biological half-life of prostacyclin in Krebs solution, human cell-free plasma or whole blood was measured by bracket assay on ADP-induced platelet aggregation. At 37°C, pH 7.4, plasma and blood reduced the rate of loss of antiaggregatory activity compared with Krebs solution. The protective effect of plasma was greater than that of whole blood. This effect could be partially mimicked by the addition of human or bovine serum albumin to the Krebs solution. The stabilisation afforded by human serum albumin was dependent on the fatty acid content of the albumin, although this was less important for bovine serum albumin.

RADIOIMMUNOASSAY OF OESTRONE AND OESTRADIOL IN THE PERIPHERAL PLASMA OF THE DOMESTIC FOWL IN VARIOUS PHYSIOLOGICAL STATES AND OF HYPOPHYSECTOMIZED AND OVARIECTOMIZED FOWLS

1974 ◽  
Vol 75 (1) ◽  
pp. 133-140 ◽  
Author(s):  
B. E. Senior
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Diethyl Ether ◽  
Bovine Serum ◽  
Domestic Fowl ◽  
Laying Hens ◽  
Peripheral Plasma ◽  
The Mean ◽  
Precision And Accuracy ◽  
Chicken Ovary

ABSTRACT A radioimmunoassay was developed to measure the levels of oestrone and oestradiol in 0.5–1.0 ml of domestic fowl peripheral plasma. The oestrogens were extracted with diethyl ether, chromatographed on columns of Sephadex LH-20 and assayed with an antiserum prepared against oestradiol-17β-succinyl-bovine serum albumin using a 17 h incubation at 4°C. The specificity, sensitivity, precision and accuracy of the assays were satisfactory. Oestrogen concentrations were determined in the plasma of birds in various reproductive states. In laying hens the ranges of oestrone and oestradiol were 12–190 pg/ml and 29–327 pg/ml respectively. Levels in immature birds, in adult cockerels and in an ovariectomized hen were barely detectable. The mean concentrations of oestrone and oestradiol in the plasma of four non-laying hens (55 pg/ml and 72 pg/ml respectively) and one partially ovariectomized hen (71 pg/ml and 134 pg/ml respectively) were well within the range for laying hens. It is evident that the large, yolk-filled follicles are not the only source of oestrogens in the chicken ovary.

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