Label-free real-time investigation of the effect of telomerase inhibitors based on quartz crystal microbalance measurement

2017 ◽  
Vol 239 ◽  
pp. 943-950 ◽  
Author(s):  
Jie Wang ◽  
Lei Liu ◽  
Hongwei Ma
Keyword(s):  
Real Time ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Label Free ◽  
Telomerase Inhibitors

scholarly journals Graphene oxide-Au nano particle coated quartz crystal microbalance biosensor for the real time analysis of carcinoembryonic antigen

RSC Advances ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 4118-4128 ◽  
Author(s):  
P. J. Jandas ◽  
Jingting Luo ◽  
Aojie Quan ◽  
Chong Li ◽  
Chen Fu ◽  
...  
Keyword(s):  
Carcinoembryonic Antigen ◽  
Real Time ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Time Estimation ◽  
Nano Particle ◽  
Label Free ◽  
Real Time Analysis ◽  
Free Quartz ◽  
Real Time Estimation

A label-free quartz crystal microbalance (QCM) biosensor was developed for the selective and real-time estimation of carcinoembryonic antigen (CEA) through the present study.

Label-free real-time acoustic sensing of microvesicle release from prostate cancer (PC3) cells using a Quartz Crystal Microbalance

2014 ◽  
Vol 453 (3) ◽  
pp. 619-624 ◽  
Author(s):  
Dan Stratton ◽  
Sigrun Lange ◽  
Sharad Kholia ◽  
Samireh Jorfi ◽  
Samuel Antwi-Baffour ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Real Time ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Label Free ◽  
Acoustic Sensing ◽  
Pc3 Cells

scholarly journals Quantitative Assessment of Periodontal Bacteria Using a Cell-Based Immunoassay with Functionalized Quartz Crystal Microbalance

Chemosensors ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 159
Author(s):  
Satit Rodphukdeekul ◽  
Miyuki Tabata ◽  
Chindanai Ratanaporncharoen ◽  
Yasuo Takeuchi ◽  
Pakpum Somboon ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Quartz Crystal Microbalance ◽  
Quantitative Assessment ◽  
Quartz Crystal ◽  
Dynamic Range ◽  
Gold Surface ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inflammatory Disorder ◽  
Label Free ◽  
Periodontal Bacteria

Periodontal disease is an inflammatory disorder that is triggered by bacterial plaque and causes the destruction of the tooth-supporting tissues leading to tooth loss. Several bacteria species, including Porphyromonas gingivalis and Aggregatibacter actinomycetemcomitans, are considered to be associated with severe periodontal conditions. In this study, we demonstrated a quartz crystal microbalance (QCM) immunoassay for quantitative assessment of the periodontal bacteria, A. actinomycetemcomitans. An immunosensor was constructed using a self-assembled monolayer of 11-mercaptoundecanoic acid (11-MUA) on the gold surface of a QCM chip. The 11-MUA layer was evaluated using a cyclic voltammetry technique to determine its mass and packing density. Next, a monoclonal antibody was covalently linked to 11-MUA using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide to act as the biorecognition element. The specificity of the monoclonal antibody was confirmed by an enzyme-linked immunosorbent assay. A calibration curve, for the relationship between the frequency shifts and number of bacteria, was used to calculate the number of A. actinomycetemcomitans bacteria in a test sample. Based on a regression equation, the lower detection limit was 800 cells, with a dynamic range up to 2.32 × 106 cells. Thus, the QCM biosensor in this study provides a sensitive and label-free method for quantitative analysis of periodontal bacteria. The method can be used in various biosensing assays for practical application and routine detection of periodontitis pathogens.

scholarly journals A Real-Time Method for Improving Stability of Monolithic Quartz Crystal Microbalance Operating under Harsh Environmental Conditions

Sensors ◽  
2021 ◽  
Vol 21 (12) ◽  
pp. 4166
Author(s):  
Román Fernández ◽  
María Calero ◽  
Yolanda Jiménez ◽  
Antonio Arnau
Keyword(s):  
Real Time ◽  
Quartz Crystal Microbalance ◽  
Quartz Crystal ◽  
Limit Of Detection ◽  
Quartz Substrate ◽  
Low Cost ◽  
Discrete Wavelet ◽  
Reagent Consumption ◽  
Measurement Cell ◽  
The Stability

Monolithic quartz crystal microbalance (MQCM) has recently emerged as a very promising technology suitable for biosensing applications. These devices consist of an array of miniaturized QCM sensors integrated within the same quartz substrate capable of detecting multiple target analytes simultaneously. Their relevant benefits include high throughput, low cost per sensor unit, low sample/reagent consumption and fast sensing response. Despite the great potential of MQCM, unwanted environmental factors (e.g., temperature, humidity, vibrations, or pressure) and perturbations intrinsic to the sensor setup (e.g., mechanical stress exerted by the measurement cell or electronic noise of the characterization system) can affect sensor stability, masking the signal of interest and degrading the limit of detection (LoD). Here, we present a method based on the discrete wavelet transform (DWT) to improve the stability of the resonance frequency and dissipation signals in real time. The method takes advantage of the similarity among the noise patterns of the resonators integrated in an MQCM device to mitigate disturbing factors that impact on sensor response. Performance of the method is validated by studying the adsorption of proteins (neutravidin and biotinylated albumin) under external controlled factors (temperature and pressure/flow rate) that simulate unwanted disturbances.

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