Plasmonic ZnO nanorods/Au substrates for protein microarrays with high sensitivity and broad dynamic range

2016 ◽  
Vol 228 ◽  
pp. 231-236 ◽  
Author(s):  
Chang Liu ◽  
Fanling Meng ◽  
Weitao Zheng ◽  
Tianyu Xue ◽  
Zhao Jin ◽  
...  
Keyword(s):  
Dynamic Range ◽  
Zno Nanorods ◽  
High Sensitivity ◽  
Protein Microarrays ◽  
Broad Dynamic Range

scholarly journals Plasmonic substrates for multiplexed protein microarrays with femtomolar sensitivity and broad dynamic range

2011 ◽  
Vol 2 (1) ◽  
Author(s):  
Scott M. Tabakman ◽  
Lana Lau ◽  
Joshua T. Robinson ◽  
Jordan Price ◽  
Sarah P. Sherlock ◽  
...  
Keyword(s):  
Dynamic Range ◽  
Protein Microarrays ◽  
Broad Dynamic Range

Ultra-sensitive detection of biomarkers and applications in pharmaceutical discovery and development. Example application to rat cardiac troponin I assay via nanoparticle probes

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. e14594-e14594
Author(s):  
P. Bao ◽  
T. Lubben ◽  
T. Holzman
Keyword(s):  
Cardiac Troponin ◽  
Troponin I ◽  
Dynamic Range ◽  
High Sensitivity ◽  
Cardiac Troponin I ◽  
Drug Induced ◽  
Drug Candidates ◽  
Broad Dynamic Range ◽  
Probe Test ◽  
Detection Of Biomarkers

e14594 Although specific to heart, rat cardiac troponin I (cTnI) is an example of an important biomarker for assessing drug-induced cardiotoxicity in animal models used in various phases of drug discovery and development. Current commercially available assays can only detect 10 ∼ 100 pg/mL in serum at the lowest limits. To improve the sensitivity of rat cTnI assay, we have developed a generically applicable, microarray based nano-probe test. Our rat cTnI assay algorithm uses a multi-step robotic process, which relies on non- isotropically oriented antibodies on functionalized glass as multiplexed microarrays to capture cTnI from serum. Functionalized, 130 angstrom diameter gold nano-probes (measured by static light scattering, 5 nm S.D.) also bind to the troponin through a molecular-scale complex containing antibodies. The troponin-bound molecular complex is then quantified through silver enhancement of the functionalized gold. Assays in this format can be rapidly configured and implemented for a wide array of potential biomarkers. For cTnI we have demonstrated a robust and ultra-sensitive assay with an LOD of less than 500 femtograms of rat cTnI per mL serum, and an overall CV of less than 20%. The assay also shows very low background, a broad dynamic range and over 3 logs of linear dose response. As an example of the potential of high sensitivity, the nanoparticle-based rat cTnI assay could significantly increase the effectiveness of measuring drug-induced heart damage at very low drug dosages and early times. Such sensitive and early measurements can improve examination of the safety of drug candidates while correspondingly reducing drug development time and cost. [Table: see text]

scholarly journals Multi-platforms approach for plasma proteomics: complementarity of Olink PEA technology to mass spectrometry-based protein profiling

2020 ◽  
Author(s):  
Agnese Petrera ◽  
Christine von Toerne ◽  
Jennifer Behler ◽  
Cornelia Huth ◽  
Barbara Thorand ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Plasma Proteins ◽  
Biomarker Discovery ◽  
Dynamic Range ◽  
High Sensitivity ◽  
Living Organism ◽  
Protein Profiling ◽  
German Population ◽  
Plasma Proteome ◽  
Broad Dynamic Range

AbstractThe plasma proteome is the ultimate target for biomarker discovery. It stores an endless amount of information on the pathophysiological status of a living organism, which is however still difficult to comprehensively access. The high structural complexity of the plasma proteome can be addressed by either a system-wide and unbiased tool such as mass spectrometry (LC-MS/MS) or a highly sensitive targeted immunoassay such as the Proximity Extension Assays (PEA). In order to address relevant differences and important shared characteristics, we tested the performance of LC-MS/MS in data-dependent and -independent acquisition modes and PEA Olink to measure circulating plasma proteins in 173 human plasma samples from a Southern German population-based cohort. We demonstrated the measurement of more than 300 proteins with both LC-MS/MS approaches applied, mainly including high abundance plasma proteins. By the use of the PEA technology, we measured 728 plasma proteins, covering a broad dynamic range with high sensitivity down to pg/ml concentrations. In a next step, we quantified 35 overlapping proteins with all three analytical platforms, verifying the reproducibility of data distributions, measurement correlation and gender-based differential expression. Our work highlights the limitations and the advantages of both, targeted and untargeted approaches, and prove their complementary strengths. We demonstrated a significant gain in proteome coverage depth and subsequent biological insight by platforms combination – a promising approach for future biomarker and mechanistic studies.

scholarly journals Reusable, Noninvasive, and Sensitive Fluorescence Enhanced ZnO-Nanorod-Based Microarrays for Quantitative Detection of AFP in Human Serum

2021 ◽  
Vol 2021 ◽  
pp. 1-11
Author(s):  
Saima Rafique ◽  
Farukh Kiyani ◽  
Sumbal Jawaid ◽  
Rubina Nasir ◽  
Mahmoosh Ahmad ◽  
...  
Keyword(s):  
Human Serum ◽  
Dynamic Range ◽  
Limit Of Detection ◽  
Zno Nanorods ◽  
Protein Microarrays ◽  
Great Promise ◽  
Quantitative Detection ◽  
Zno Nanorod ◽  
Serum Samples ◽  
Protein Assay

The fabrication of sensitive protein microarrays such as PCR used in DNA microarray is challenging due to lack of signal amplification. The development of microarrays is utilized to improve the sensitivity and limitations of detection towards primal cancer detection. The sensitivity is enhanced by the use of ZnO-nanorods and is investigated as a substrate which enhance the florescent signal to diagnose the hepatocellular carcinoma (HCC) at early stages. The substrate for deposition of ZnO-nanorods is prepared by the conventional chemical bath deposition method. The resultant highly dense ZnO-nanorods enhance the fluorescent signal 7.2 times as compared to the substrate without ZnO-nanorods. The microarray showed sensitivity of 1504.7 ng ml-1 and limit of detection of 0.1 pg ml-1 in wide dynamic range of 0.05 pg-10 μg ml-1 for alpha fetoprotein (AFP) detection in 10% human serum. This immunoassay was successfully applied for human serum samples to detect tumor marker with good recoveries. The ZnO-nanorod substrate is a simple protein microarray which showed a great promise for developing a low-cost, sensitive, and high-throughput protein assay platform for several applications in both fundamental research and clinical diagnosis.

scholarly journals Conformational pathway provides unique sensitivity to a synaptic mGluR

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Chris H. Habrian ◽  
Joshua Levitz ◽  
Vojtech Vyklicky ◽  
Zhu Fu ◽  
Adam Hoagland ◽  
...  
Keyword(s):  
Single Molecule ◽  
Metabotropic Glutamate Receptors ◽  
Dynamic Range ◽  
High Sensitivity ◽  
G Protein Coupled Receptors ◽  
Metabotropic Glutamate ◽  
Single Molecule Fret ◽  
Broad Dynamic Range ◽  
Maximal Activation ◽  
G Protein Coupled

AbstractMetabotropic glutamate receptors (mGluRs) are dimeric G-protein–coupled receptors that operate at synapses. Macroscopic and single molecule FRET to monitor structural rearrangements in the ligand binding domain (LBD) of the mGluR7/7 homodimer revealed it to have an apparent affinity ~4000-fold lower than other mGluRs and a maximal activation of only ~10%, seemingly too low for activation at synapses. However, mGluR7 heterodimerizes, and we find it to associate with mGluR2 in the hippocampus. Strikingly, the mGluR2/7 heterodimer has high affinity and efficacy. mGluR2/7 shows cooperativity in which an unliganded subunit greatly enhances activation by agonist bound to its heteromeric partner, and a unique conformational pathway to activation, in which mGluR2/7 partially activates in the Apo state, even when its LBDs are held open by antagonist. High sensitivity and an unusually broad dynamic range should enable mGluR2/7 to respond to both glutamate transients from nearby release and spillover from distant synapses.

scholarly journals Ozone Detection by Crack-Induced Opacity in Rubber

1999 ◽  
Vol 72 (4) ◽  
pp. 769-778 ◽  
Author(s):  
P. H. Mott ◽  
C. M. Roland
Keyword(s):  
Dynamic Range ◽  
Crack Nucleation ◽  
High Sensitivity ◽  
Nucleation And Growth ◽  
Atmospheric Ozone ◽  
Surface Cracks ◽  
Broad Dynamic Range ◽  
Consequent Reduction ◽  
Ozone Detection ◽  
Crack Nucleation And Growth

Abstract Initially transparent polybutadiene develops micron-sized surface cracks when stretched and exposed to ozone. The consequent reduction in the transparency of the rubber provides a facile method for quantifying the ambient ozone concentration. The rate at which opacity develops is linearly dependent on the amount of ozone, and increases with increasing strain. This method of detecting atmospheric ozone has high sensitivity (1 ppb), a broad dynamic range, and is unaffected by the presence of other chemicals. The surface morphology of exposed material can be interpreted in terms of crack nucleation and growth.

High Sensitivity Plasmonic Sensing of Hydrogen over a Broad Dynamic Range Using Catalytic Au-CeO2 Thin Film Nanocomposites

ACS Sensors ◽  
2018 ◽  
Vol 3 (12) ◽  
pp. 2684-2692 ◽  
Author(s):  
Nora M. Houlihan ◽  
Nicholas Karker ◽  
Radislav A. Potyrailo ◽  
Michael A. Carpenter
Keyword(s):  
Thin Film ◽  
Dynamic Range ◽  
High Sensitivity ◽  
Plasmonic Sensing ◽  
Broad Dynamic Range ◽  
Film Nanocomposites

scholarly journals A Bioinspired Tilt Sensor Model with Adaptive Gain and Enhanced Sensitivity

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Lijun Liu ◽  
Ying Lei
Keyword(s):  
Dynamic Range ◽  
Inertial Sensors ◽  
High Sensitivity ◽  
Negative Stiffness ◽  
Mechanical Stimuli ◽  
Enhanced Sensitivity ◽  
Sensor Model ◽  
Broad Dynamic Range ◽  
Tilt Sensor ◽  
Tilt Sensors

Although various types of tilt sensors have been proposed in the past decade, it is still essential to develop rugged, cheap, simple-structured tilt sensors with wide measuring range and high sensitivities for efficient monitoring of infrastructures and early warning of natural disasters. It has been investigated that stereocilia in some fishes’ inner ear organs are the basic sensory units of nature’s inertial sensors and are highly sensitive over broad dynamic range because of a combination of adaptation and negative stiffness mechanisms. In this paper, a bioinspired tilt sensor model is proposed that mimics the mechanism of stereocilia in adaptive signal amplification to mechanical stimuli, leading to high sensitivity to weak input and low sensitivity to high input, thus expanding the dynamic range through adaptive amplification. The negative stiffness mechanism is implemented by magnet forces. The tilt motion is measured by the strain gauge at the end of the flexible cantilever beam element in the model. Measurements of both static and dynamics tilt motion are investigated. Numerical simulation results are used to demonstrate the capability of the proposed model for the measurements of tilt motions with adaptive amplification and enhanced sensitivity.

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