A pinch-valve for centrifugal microfluidic platforms and its application in sequential valving operation and plasma extraction

2015 ◽  
Vol 221 ◽  
pp. 257-264 ◽  
Author(s):  
Ziliang Cai ◽  
Jiwen Xiang ◽  
Wanjun Wang
Keyword(s):  
Microfluidic Platforms ◽  
Pinch Valve ◽  
Plasma Extraction

Extraction of Protein-Bound ATP and ADP from Human Platelets in Plasma

1990 ◽  
Vol 63 (02) ◽  
pp. 286-290 ◽  
Author(s):  
Christina Beurling-Harbury ◽  
Pehr B Harbury
Keyword(s):  
Specific Activity ◽  
Platelet Rich Plasma ◽  
Binding Protein ◽  
Human Platelets ◽  
Luciferase Assay ◽  
First Time ◽  
Plasma Extraction

SummaryActin is the major ATP and ADP binding protein in platelets, 0.9–1.3 nmol/108 cells, 50–70% in the unpolymerized state. The goal of these experiments was to develop a method for extracting all protein-bound ATP and ADP from undisturbed platelets in plasma. Extraction of actin-bound ADP is routine while extraction of actin-bound ATP from platelets in buffer has been unsuccessful. Prior to extraction the platelets were exposed to 14-C adenine, to label the metabolic and actin pools of ATP and ADP. The specific activity was determined from the actin-bound ADP in the 43% ethanol precipitate. Sequential ethanol and perchlorate extractions of platelet rich plasma, and the derived supernatants and precipitates were performed. ATP concentrations were determined with the luciferase assay, and radioactive nucleotides separated by TLC. A total of 1.18 nmol/108 cells of protein-bound ATP and ADP was recovered, 52% ATP (0.61 nmol). The recovery of protein-bound ADP was increased from 0.3 to 0.57 nmol/108 cells. This approach for the first time successfully recovered protein bound ATP and ADP from platelets in a concentration expected for actin.

Proposal of Micro Plasma Extraction Device by Autonomous Trigger Control

2020 ◽  
Vol 140 (4) ◽  
pp. 437-442
Author(s):  
Hiroki Naito ◽  
Shunya Okamoto ◽  
Yoshiaki Ukita
Keyword(s):  
Extraction Device ◽  
Plasma Extraction

Magnetic Digital Microfluidics for Point-of-Care Testing: Where Are We Now?

2020 ◽  
Vol 27 ◽  
Author(s):  
Yi Zhang
Keyword(s):  
System Integration ◽  
Diagnostic Tests ◽  
Point Of Care ◽  
Digital Microfluidics ◽  
Controlled Environment ◽  
Point Of Care Testing ◽  
Microfluidic Platforms ◽  
Interface System ◽  
Technical Issues ◽  
Sample System

: Point-of-care (POC) testing decentralizes the diagnostic tests to the sites near the patient. Many POC tests rely microfluidic platforms for sample-to-answer analysis. Compared to other microfluidic systems, magnetic digital microfluidics demonstrate compelling advantages for POC diagnostics. In this review, we have examined the capability of magnetic digital microfluidics-based POC diagnostic platforms. More importantly, we have categorized POC settings into three classes based on “where is the point”, “who to care” and “how to test”, and evaluated the suitability of magnetic digital microfluidics in various POC settings. Furthermore, we have addressed other technical issues associated with POC testing such as controlled environment, sample-system interface, system integration and information connectivity. We hope this review would provide a guideline for the future development of magnetic digital microfluidics-based platforms for POC testing.

On-chip anticancer drug screening – Recent progress in microfluidic platforms to address challenges in chemotherapy

2019 ◽  
Vol 137 ◽  
pp. 236-254 ◽  
Author(s):  
Nandini Dhiman ◽  
Peter Kingshott ◽  
Huseyin Sumer ◽  
Chandra S. Sharma ◽  
Subha Narayan Rath
Keyword(s):  
Anticancer Drug ◽  
Drug Screening ◽  
Recent Progress ◽  
Microfluidic Platforms ◽  
On Chip

Engineering Tissue Barrier Models on Hydrogel Microfluidic Platforms

2021 ◽  
Author(s):  
Daniel Vera ◽  
María García-Díaz ◽  
Núria Torras ◽  
Mar Álvarez ◽  
Rosa Villa ◽  
...  
Keyword(s):  
Microfluidic Platforms

scholarly journals Homogeneous Freezing of Water Using Microfluidics

Micromachines ◽  
2021 ◽  
Vol 12 (2) ◽  
pp. 223
Author(s):  
Mark D. Tarn ◽  
Sebastien N. F. Sikora ◽  
Grace C. E. Porter ◽  
Jung-uk Shim ◽  
Benjamin J. Murray
Keyword(s):  
Nucleation Theory ◽  
Classical Nucleation Theory ◽  
Recent Theory ◽  
Water Droplets ◽  
Microfluidic Platforms ◽  
High Throughput Method ◽  
Theoretical Predictions ◽  
New Instrumentation ◽  
Controlled Environments ◽  
Homogeneous Freezing

The homogeneous freezing of water is important in the formation of ice in clouds, but there remains a great deal of variability in the representation of the homogeneous freezing of water in the literature. The development of new instrumentation, such as droplet microfluidic platforms, may help to constrain our understanding of the kinetics of homogeneous freezing via the analysis of monodisperse, size-selected water droplets in temporally and spatially controlled environments. Here, we evaluate droplet freezing data obtained using the Lab-on-a-Chip Nucleation by Immersed Particle Instrument (LOC-NIPI), in which droplets are generated and frozen in continuous flow. This high-throughput method was used to analyse over 16,000 water droplets (86 μm diameter) across three experimental runs, generating data with high precision and reproducibility that has largely been unrepresented in the microfluidic literature. Using this data, a new LOC-NIPI parameterisation of the volume nucleation rate coefficient (JV(T)) was determined in the temperature region of −35.1 to −36.9 °C, covering a greater JV(T) compared to most other microfluidic techniques thanks to the number of droplets analysed. Comparison to recent theory suggests inconsistencies in the theoretical representation, further implying that microfluidics could be used to inform on changes to parameterisations. By applying classical nucleation theory (CNT) to our JV(T) data, we have gone a step further than other microfluidic homogeneous freezing examples by calculating the stacking-disordered ice–supercooled water interfacial energy, estimated to be 22.5 ± 0.7 mJ m−2, again finding inconsistencies when compared to theoretical predictions. Further, we briefly review and compile all available microfluidic homogeneous freezing data in the literature, finding that the LOC-NIPI and other microfluidically generated data compare well with commonly used non-microfluidic datasets, but have generally been obtained with greater ease and with higher numbers of monodisperse droplets.

scholarly journals On the Wireless Microwave Sensing of Bacterial Membrane Potential in Microfluidic-Actuated Platforms

Sensors ◽  
2021 ◽  
Vol 21 (10) ◽  
pp. 3420
Author(s):  
Marc Jofre ◽  
Lluís Jofre ◽  
Luis Jofre-Roca
Keyword(s):  
Membrane Potential ◽  
Living Cells ◽  
Membrane Potentials ◽  
Electromagnetic Properties ◽  
Bacterial Membrane ◽  
Microfluidic Platforms ◽  
Bacterial Membranes ◽  
Wireless Monitoring ◽  
Bacterial Dynamics ◽  
Engineered Systems

The investigation of the electromagnetic properties of biological particles in microfluidic platforms may enable microwave wireless monitoring and interaction with the functional activity of microorganisms. Of high relevance are the action and membrane potentials as they are some of the most important parameters of living cells. In particular, the complex mechanisms of a cell’s action potential are comparable to the dynamics of bacterial membranes, and consequently focusing on the latter provides a simplified framework for advancing the current techniques and knowledge of general bacterial dynamics. In this work, we provide a theoretical analysis and experimental results on the microwave detection of microorganisms within a microfluidic-based platform for sensing the membrane potential of bacteria. The results further advance the state of microwave bacteria sensing and microfluidic control and their implications for measuring and interacting with cells and their membrane potentials, which is of great importance for developing new biotechnologically engineered systems and solutions.

Microfluidic Platforms toward Rational Material Fabrication for Biomedical Applications

Small ◽  
2019 ◽  
Vol 16 (9) ◽  
pp. 1903798 ◽  
Author(s):  
Qilong Zhao ◽  
Huanqing Cui ◽  
Yunlong Wang ◽  
Xuemin Du
Keyword(s):  
Biomedical Applications ◽  
Microfluidic Platforms ◽  
Material Fabrication

scholarly journals Surface acoustic wave devices for chemical sensing and microfluidics: a review and perspective

2017 ◽  
Vol 9 (28) ◽  
pp. 4112-4134 ◽  
Author(s):  
David B. Go ◽  
Massood Z. Atashbar ◽  
Zeinab Ramshani ◽  
Hsueh-Chia Chang
Keyword(s):  
Acoustic Wave ◽  
Surface Acoustic Wave ◽  
Chemical Sensing ◽  
Chemical Detection ◽  
Microfluidic Platforms ◽  
Acoustic Wave Sensors ◽  
Acoustic Wave Devices ◽  
Wave Sensors

Surface acoustic wave sensors and microfluidic platforms enable effective chemical detection and sample manipulation.

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