scholarly journals Laser-induced fluorescence integrated in a microfluidic immunosensor for quantification of human serum IgG antibodies to Helicobacter pylori

2012 ◽  
Vol 168 ◽  
pp. 297-302 ◽  
Author(s):  
Marco A. Seia ◽  
Sirley V. Pereira ◽  
Carlos A. Fontán ◽  
Irma E. De Vito ◽  
Germán A. Messina ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Human Serum ◽  
Laser Induced Fluorescence ◽  
Igg Antibodies ◽  
Serum Igg

Continuous-flow/stopped-flow system using an immunobiosensor for quantification of human serum IgG antibodies to Helicobacter pylori

2005 ◽  
Vol 337 (2) ◽  
pp. 195-202 ◽  
Author(s):  
Germán A. Messina ◽  
Angel A.J. Torriero ◽  
Irma E. De Vito ◽  
Roberto A. Olsina ◽  
Julio Raba
Keyword(s):  
Helicobacter Pylori ◽  
Human Serum ◽  
Continuous Flow ◽  
Flow System ◽  
Stopped Flow ◽  
Igg Antibodies ◽  
Serum Igg

Immuno-column for on-line quantification of human serum IgG antibodies to Helicobacter pylori in human serum samples

Talanta ◽  
2008 ◽  
Vol 76 (5) ◽  
pp. 1077-1082 ◽  
Author(s):  
Luis Molina ◽  
Germán A. Messina ◽  
Patrícia W. Stege ◽  
Eloy Salinas ◽  
Julio Raba
Keyword(s):  
Helicobacter Pylori ◽  
Human Serum ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Human Serum Samples ◽  
Serum Igg ◽  
On Line

Noninvasive evaluation of Helicobacter pylori therapy: role of fasting or postprandial gastrin, Pepsinogen I, Pepsinogen II, or serum IgG antibodies

1999 ◽  
Vol 94 (9) ◽  
pp. 2367-2372 ◽  
Author(s):  
M. Tarek Al-Assi ◽  
Kazumasa Miki ◽  
John H. Walsh ◽  
David P. Graham ◽  
Masahiro Asaka ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Noninvasive Evaluation ◽  
Igg Antibodies ◽  
Pepsinogen I ◽  
Serum Igg ◽  
Pepsinogen Ii

Peroral immunization with Helicobacter pylori adhesin protein genetically linked to cholera toxin A2B subunits

2001 ◽  
Vol 100 (3) ◽  
pp. 291-298 ◽  
Author(s):  
Byung Oh KIM ◽  
Sung Seup SHIN ◽  
Young Hyo YOO ◽  
Suhkneung PYO
Keyword(s):  
Helicobacter Pylori ◽  
Cholera Toxin ◽  
Vaccine Development ◽  
Protective Antigen ◽  
Oral Immunization ◽  
Size Exclusion ◽  
Vaccine Antigen ◽  
Igg Antibodies ◽  
Serum Igg ◽  
H Pylori

Helicobacter pylori is a major cause of gastric-associated diseases. To evaluate the efficacy of a possible vaccine antigen against H. pylori infection, the chimaeric construct adhesin–CTXA2B, derived from H. pylori adhesin genetically coupled to cholera toxin (CTX) subunits A2 and B (CTXA2B), was expressed in Escherichia coli as an insoluble recombinant chimaeric protein. The protein was then purified by denaturation, renaturation and size-exclusion chromatography. The composition of purified adhesin–CTXA2B was verified by SDS/PAGE and Western blotting with antibodies to antigenic components of adhesin and CTXB, and confirmed as a chimaeric protein with GM1-ganglioside binding activity and adhesin epitopes by a GM1-ELISA developed using antibodies to adhesin. Oral immunization of mice with adhesin–CTXA2B induced higher levels of mucosal IgA and serum IgG antibodies to H. pylori adhesin and to CTXB than in mice immunized with adhesin or CTXA2B alone. Adhesin–CTXA2B was also demonstrated to be a potential protective antigen in a mouse model of H. pylori infection. The immunization of mice with adhesin–CTXA2B protected 62.5% of mice infected with H. pylori SS1 strain, whereas adhesin immunization was not able to confer protection to mice. This protection may be correlated with high levels of mucosal IgA and serum IgG antibodies against H. pylori adhesin. Taken together, the results indicate that the genetically linked CTXA2B acts as a useful mucosal adjuvant, and that the adhesin–CTXA2B chimaeric protein could be a potential component in future H. pylori vaccine development.

scholarly journals Pre-existing Helicobacter pylori serum IgG enhances the vibriocidal antibody response to CVD 103-HgR live oral cholera vaccine in Malian adults

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Khitam Muhsen ◽  
Samba O. Sow ◽  
Milagritos D. Tapia ◽  
Fadima C. Haidara ◽  
Mardi Reymann ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Oral Vaccine ◽  
Adjusted Relative Risk ◽  
Cholera Vaccine ◽  
Igg Antibodies ◽  
Fourfold Increase ◽  
Serum Igg ◽  
Serum Pepsinogens ◽  
Oral Cholera Vaccine ◽  
H Pylori

Abstract Accumulating evidence indicates that persistent Helicobacter pylori gastric infection influences immune responses to oral enteric vaccines. We studied the association between pre-existing H. pylori serum IgG and serum pepsinogens levels (PGs) as markers of gastric inflammation and the immune response to single-dose live oral cholera vaccine CVD 103-HgR in Malian adults. Baseline sera obtained during a phase 2 safety/immunogenicity clinical trial of cholera vaccine CVD 103-HgR among 93 healthy Malian adults were tested for H. pylori IgG antibodies and PGI and PGII levels using enzyme linked immunosorbent assays. Overall 74/93 (80%) vaccine recipients were H. pylori IgG seropositive at baseline. Vibriocidal antibody seroconversion (≥ fourfold increase 14 days following administration of CVD 103-HgR compared to baseline) among vaccine recipients was 56%. However, vibriocidal antibody seroconversion was markedly higher among H. pylori seropositives than seronegatives 64% vs. 26% (p = 0.004); adjusted relative risk: 2.20 (95% confidence intervals 1.00–4.80; p = 0.049). Among H. pylori seropositive vaccine recipients, there were no significant associations between PGI, PGII and PGI:PGII levels and vibriocidal seroconversion. The enhanced seroconversion to oral cholera vaccine CVD 103-HgR among H. pylori seropositive African adults provides further evidence of the immunomodulating impact of H. pylori on oral vaccine immunogenicity.

Testing for Serum IgG Antibodies to Helicobacter pylori Cytotoxin-Associated Protein Detects Children with Higher Grades of Gastric Inflammation

1999 ◽  
Vol 29 (3) ◽  
pp. 302-307 ◽  
Author(s):  
Francesco Luzza ◽  
Antonio Contaldo ◽  
Maria Imeneo ◽  
Maria Mancuso ◽  
Licia Pensabene ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Igg Antibodies ◽  
Serum Igg
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