An amperometric glucose biosensor based on layer-by-layer GOx-SWCNT conjugate/redox polymer multilayer on a screen-printed carbon electrode

2011 ◽  
Vol 153 (1) ◽  
pp. 219-225 ◽  
Author(s):  
Qiang Gao ◽  
Yanyan Guo ◽  
Wenyan Zhang ◽  
Honglan Qi ◽  
Chengxiao Zhang
2012 ◽  
Vol 571 ◽  
pp. 56-59
Author(s):  
Yu Fang Sha ◽  
Mei Zhao ◽  
Ming Quan Yang ◽  
Hai Xin Bai ◽  
Man Zhao

Biological multilayer films of redox polymer and horseradish peroxidase (HRP) were successfully assembled on a screen-printed carbon electrode using layer-by-layer (LBL) assembled method based on the electrostatic interaction. The screen-printed carbon electrode surface was modified by the positively charged redox polymer, and the negatively charged HRP by LBL method.


2013 ◽  
Vol 341-342 ◽  
pp. 307-310
Author(s):  
Yu Fang Sha ◽  
Hong Jun Lan ◽  
Yan Zhang ◽  
Shou Shuo Liu ◽  
Jun Xie

Using layer-by-layer (LBL) assembled method, multilayer films containing multiwall carbon nanotubes (MWNTs) and redox polymer were successfully fabricated on a screen-printed carbon electrode. X-ray photoelectron spectroscopy (XPS) and field-emission scanning electron microscopy (FE-SEM) were used to characterize the assembled multilayer films.


2013 ◽  
Vol 341-342 ◽  
pp. 221-224
Author(s):  
Yu Fang Sha ◽  
Yan Chun Hu ◽  
De Xiang Sun ◽  
Yong Sheng Guo ◽  
Bing Zhang

Multilayer films containing carbon nanotubes and redox polymer were successfully fabricated on a screen-printed carbon electrode using layer-by-layer (LBL) assembled method. UV-vis spectroscopy and electrochemical method were used to characterize the assembled multilayer films.


2012 ◽  
Vol 571 ◽  
pp. 538-541
Author(s):  
Yu Fang Sha ◽  
Mei Zhao ◽  
Fei Guo ◽  
Jun Xie ◽  
Wei Han

Improvement of sensitivity of electrochemical sandwich enzyme immunoassay has been achieved by electrodepositing redox polymer on screen-printed carbon electrode surface, on which sandwich complex were formed.


2021 ◽  
Vol 24 (2) ◽  
pp. 43-50
Author(s):  
Wulan Tri Wahyuni ◽  
Rudi Heryanto ◽  
Eti Rohaeti ◽  
Achmad Fauzi ◽  
Budi Riza Putra

A screen-printed carbon electrode is a suitable electrode for electrochemical sensors due to its simplicity and portability. This study aimed to fabricate a screen-printed carbon electrode modified with poly (3,4-ethylenedioxythiophene) polystyrene sulfonate (SPCE-PEDOT:PSS) to improve the electrochemical performance for uric acid detection. The SPCE was fabricated using a layer-by-layer painting process of conductive ink consisting of graphite as a conductive material, polystyrene as a polymeric binder, and dichloromethane solvent on a polyvinyl chloride paper substrate. The fabricated SPCE was then modified with PEDOT:PSS by a drop-casting method. The characterization of SPCE-PEDOT:PSS surface morphology was performed using the scanning electron microscopy technique. The SPCE-PEDOT:PSS provided an acceptable linearity (R2 = 0.9985, 0.9993, 0.9985), sensitivity (0.070, 0.015, 0.024 µA/µM), precision (%RSD = 2.70%, 2.89%, 2.40%), limit of detection (1.61 µM, 1.14 µM, 1.62 µM), and limit of quantitation (5.37 µM, 3.81 µM to 5.39 µM) in measurement of uric acid standard solution using cyclic voltammetry, amperometry, and differential pulse voltammetry techniques, respectively. The studies using SPCE-PEDOT:PSS indicated that the electrode could be applied in the electrochemical measurement of uric acid in the human urine sample.


2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Vojtěch Polan ◽  
Jan Soukup ◽  
Karel Vytřas

The described glucose biosensor is based on a screen-printed carbon electrode (SPCE) modified by rhodium dioxide, which functions as a mediator. The electrode is further modified by the enzyme glucose dehydrogenase, which is immobilized on the electrode's surface through electropolymerization with m-phenylenediamine. The enzyme biosensor was optimized and tested in model glucose samples. The biosensor showed a linear range of 500–5000 mg L−1 of glucose with a detection limit of 210 mg L−1 (established as 3σ) and response time of 39 s. When compared with similar glucose biosensors based on glucose oxidase, the main advantage is that neither ascorbic and uric acids nor paracetamol interfere measurements with this biosensor at selected potentials.


2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Azizul Isha ◽  
Fowotade Sulayman Akanbi ◽  
Nor Azah Yusof ◽  
Rosiah Osman ◽  
Wong Mui-Yun ◽  
...  

Ganoderma boninense (G. boninense) has been identified as a major problem in oil palm industry which caused basal stem rot disease. Identification of metabolite variation of healthy and G. boninense-infected oil palm leaves at 14 days postinfection using NMR metabolomics approach followed by characterization of an electrochemical sensor based on a functionalized multiwalled carbon nanotube (MWCNT) layer-by-layer framework on modified screen-printed carbon electrode has been successfully determined. Significant differences from the 1H NMR data were observed between healthy and G. boninense-infected oil palm leaves, according to principal component analysis. Gold nanoparticle-functionalized MWCNT and chitosan-functionalized MWCNT were deposited on a screen-printed carbon electrode and were applied for the electrochemical detection of healthy and G. boninense-infected oil palm leaves. The electrocatalytic activities of a modified electrode towards oxidation of healthy and G. boninense-infected oil palm leaves at a concentration of 100 mg/L were evaluated using cyclic voltammetry and linear sweep voltammetry. The limits of detection of healthy and G. boninense-infected oil palm leaves were calculated to 0.0765 mg/L and 0.0414 mg/L, respectively. The modified electrode shows a good sensitivity and reproducibility due to the unique characteristics of gold nanoparticles, chitosan, MWCNTs, and synergistic interaction between them.


2012 ◽  
Vol 4 (3) ◽  
pp. 172-179
Author(s):  
Ashok K. Sundramoorthy ◽  
Szu-Ling Chen ◽  
Shen-Ming Chen

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