Gold nanoparticle accumulation using magnetic particles: A new strategy for electrochemical immunoassay based on the reversible reaction between dethiobiotin and avidin

2007 ◽  
Vol 123 (1) ◽  
pp. 198-203 ◽  
Author(s):  
Xun Mao ◽  
Jianhui Jiang ◽  
Yong Huang ◽  
Guoli Shen ◽  
Ruqin Yu
Keyword(s):  
Gold Nanoparticle ◽  
Magnetic Particles ◽  
Reversible Reaction ◽  
Electrochemical Immunoassay ◽  
New Strategy

Cyclic accumulation of nanoparticles: A new strategy for electrochemical immunoassay based on the reversible reaction between dethiobiotin and avidin

2006 ◽  
Vol 557 (1-2) ◽  
pp. 159-163 ◽  
Author(s):  
Xun Mao ◽  
Jianhui Jiang ◽  
Jiwei Chen ◽  
Yong Huang ◽  
Guoli Shen ◽  
...  
Keyword(s):  
Reversible Reaction ◽  
Electrochemical Immunoassay ◽  
New Strategy

A new strategy for electrochemical immunoassay based on enzymatic silver deposition on agarose beads

Talanta ◽  
2008 ◽  
Vol 74 (5) ◽  
pp. 1642-1648 ◽  
Author(s):  
Yan Luo ◽  
Xun Mao ◽  
Zhao-Feng Peng ◽  
Jian-Hui Jiang ◽  
Guo-Li Shen ◽  
...  
Keyword(s):  
Electrochemical Immunoassay ◽  
Silver Deposition ◽  
Agarose Beads ◽  
New Strategy

Surface modification of bacterial magnetic nanoparticles using artificial polypeptides consisting of a repeated asparagine-serine dipeptide and a transmembrane peptide

2012 ◽  
Vol 1464 ◽  
Author(s):  
Toru Honda ◽  
Tomoko Yoshino ◽  
Tsuyoshi Tanaka ◽  
Tadashi Matsunaga
Keyword(s):  
Aqueous Solution ◽  
Surface Modification ◽  
Magnetic Nanoparticles ◽  
Magnetic Particles ◽  
Specific Binding ◽  
Nanoparticle Surface ◽  
New Strategy ◽  
Modification Technique ◽  
Bacterial Magnetic Particles ◽  
Nanoparticle Surface Modification

ABSTRACTSurface modification is an important part of fabricating nanoparticles with specific properties and functions. We have designed a dipeptide, which we call NS polypeptide, that consists of four asparagine (N) residues and one serine (S) residue, as a molecule for nanoparticle surface modification. Surface modification of magnetic nanoparticles with the NS polypeptide results in reduction of particle-particle and particle-cell interactions. Here, we describe the surface modification and functionalization of bacterial magnetic particles (BacMPs) by spontaneous integration of temporin L conjugated to NS polypeptide. BacMP membranes were modified temporin L. Furthermore, peptide-modified BacMPs showed high dispersibility in aqueous solution compared to unmodified BacMPs. This surface modification technique may represent a new strategy for reducing non-specific binding of nanoparticles to proteins or cells for use in a variety of protein- or cell-associated applications.

scholarly journals Glyphosate Determination by Coupling an Immuno-Magnetic Assay with Electrochemical Sensors

Sensors ◽  
2018 ◽  
Vol 18 (9) ◽  
pp. 2965 ◽  
Author(s):  
Francesca Bettazzi ◽  
Aline Romero Natale ◽  
Eduardo Torres ◽  
Ilaria Palchetti
Keyword(s):  
Magnetic Particles ◽  
Electrochemical Sensors ◽  
Electrochemical Cell ◽  
Electrochemical Immunoassay ◽  
Competitive Immunoassay ◽  
Quantification Limit ◽  
Intensive Use ◽  
Current Value ◽  
Global Contamination ◽  
Enzymatic Product

Glyphosate (N-(phosphonomethyl)glycine) is the most frequently used broad-spectrum herbicide worldwide. Its mechanism of action is based on the inhibition of an enzyme that is essential to plant growth. Its intensive use has caused global contamination to occur, which has not only affected the ecosystems, but even food and other objects of common use. Thus, there is a pronounced need for developing analytical methods for glyphosate determination in different matrices. Here, an electrochemical competitive immunoassay, based on the use of antibody-modified magnetic particles, has been developed. Tetramethylbenzidine (TMB) has been used as an enzymatic substrate. The extent of the affinity reaction has been achieved by monitoring the current value, due to the reduction of the enzymatic product. A disposable screen-printed electrochemical cell has been used. The calibration curve has been recorded in the 0–10,000 ng/L concentration range, with a detection limit of 5 ng/L and quantification limit of 30 ng/L. The electrochemical immunoassay has also been applied to the analysis of spiked beer samples.

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