Characterization of CD8+ T cell repertoire diversity and persistence in the influenza A virus model of localized, transient infection

2004 ◽  
Vol 16 (3) ◽  
pp. 179-184 ◽  
Author(s):  
Stephen J Turner ◽  
Katherine Kedzierska ◽  
Nicole L La Gruta ◽  
Richard Webby ◽  
Peter C Doherty
Keyword(s):  
T Cell ◽  
Influenza A Virus ◽  
Influenza A ◽  
Cd8 T Cell ◽  
T Cell Repertoire ◽  
Cell Repertoire ◽  
Repertoire Diversity ◽  
Virus Model

scholarly journals Characterization of human cytomegalovirus peptide–specific CD8+ T-cell repertoire diversity following in vitro restimulation by antigen-pulsed dendritic cells

Blood ◽  
2002 ◽  
Vol 99 (1) ◽  
pp. 213-223 ◽  
Author(s):  
Karl Peggs ◽  
Stephanie Verfuerth ◽  
Arnold Pizzey ◽  
Jenni Ainsworth ◽  
Paul Moss ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
T Cell ◽  
Viral Replication ◽  
Cd8 T Cells ◽  
Cd8 T Cell ◽  
T Cell Repertoire ◽  
Cell Repertoire ◽  
Repertoire Diversity ◽  
Clonal Composition

Under conditions of impaired T-cell immunity, human cytomegalovirus (HCMV) can reactivate from lifelong latency, resulting in potentially fatal disease. A crucial role for CD8+ T cells has been demonstrated in control of viral replication, and high levels of HCMV-specific cytotoxic T-lymphocytes are seen in immunocompetent HCMV-seropositive individuals despite very low viral loads. Elucidation of the minimum portion of the anti-HCMV T-cell repertoire that is required to suppress viral replication requires further study of clonal composition. The ability of dendritic cells to take up and process exogenous viral antigen by constitutive macropinocytosis was used to study HCMV-specific T-cell memory in the absence of viral replication. The specificity and clonal composition of the CD8+ T-cell responses were evaluated using HLA tetrameric complexes and T-cell receptor β chain (TCRBV) spectratypic analyses. There was a skewed reactivity toward the matrix protein pp65, with up to 40-fold expansion of CD8+ T cells directed toward a single peptide-MHC combination. Individual expansions detected on TCRBV spectratype analysis were HCMV-specific and composed of single or highly restricted numbers of clones. There was preferential TCRBV gene usage (BV6.1/6.2, BV8, and BV13 in HLA-A*0201+ individuals) but lack of conservation of CDR3 length and junctional motifs between donors. While there was a spectrum of TCR repertoire diversity directed toward individual MHC-peptide combinations between donors, a relatively small number of clones appeared to predominate the response in each case. These data provide further insight into the range of anti-HCMV responses and will aid the design and monitoring of adoptive immunotherapy protocols.

scholarly journals Cytomegalovirus Exposure in the Elderly Does Not Reduce CD8 T Cell Repertoire Diversity

2018 ◽  
Vol 202 (2) ◽  
pp. 476-483 ◽  
Author(s):  
Paul Lindau ◽  
Rithun Mukherjee ◽  
Miriam V. Gutschow ◽  
Marissa Vignali ◽  
Edus H. Warren ◽  
...  
Keyword(s):  
T Cell ◽  
The Elderly ◽  
Cd8 T Cell ◽  
T Cell Repertoire ◽  
Cell Repertoire ◽  
Repertoire Diversity

scholarly journals Cytomegalovirus Exposure in the Elderly Does Not Reduce CD8 T Cell Repertoire Diversity

immuneACCESS ◽  
2019 ◽  
Author(s):  
P Lindau ◽  
R Mukherjee ◽  
MV Gutschow ◽  
M Vignali ◽  
EH Warren ◽  
...  
Keyword(s):  
T Cell ◽  
The Elderly ◽  
Cd8 T Cell ◽  
T Cell Repertoire ◽  
Cell Repertoire ◽  
Repertoire Diversity

scholarly journals Characterization of CD8+ T cell repertoire in identical twins discordant and concordant for multiple sclerosis

2006 ◽  
Vol 81 (3) ◽  
pp. 696-710 ◽  
Author(s):  
Paolo Somma ◽  
Giovanni Ristori ◽  
Luca Battistini ◽  
Stefania Cannoni ◽  
Giovanna Borsellino ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
T Cell ◽  
Cd8 T Cell ◽  
Identical Twins ◽  
T Cell Repertoire ◽  
Cell Repertoire

scholarly journals Human CD4+ T-cell repertoire of responses to influenza A virus hemagglutinin after recent natural infection.

1995 ◽  
Vol 69 (12) ◽  
pp. 7497-7506 ◽  
Author(s):  
C M Gelder ◽  
K I Welsh ◽  
A Faith ◽  
J R Lamb ◽  
B A Askonas
Keyword(s):  
T Cell ◽  
Influenza A Virus ◽  
Influenza A ◽  
Natural Infection ◽  
Cd4 T Cell ◽  
T Cell Repertoire ◽  
Cell Repertoire

scholarly journals Molecular and flow cytometric characterization of the CD4 and CD8 T-cell repertoire in patients with myelodysplastic syndrome

2002 ◽  
Vol 119 (1) ◽  
pp. 97-105 ◽  
Author(s):  
J. Joseph Melenhorst ◽  
Rhoda Eniafe ◽  
Dean Follmann ◽  
Ryo Nakamura ◽  
Martha Kirby ◽  
...  
Keyword(s):  
T Cell ◽  
Myelodysplastic Syndrome ◽  
Cd8 T Cell ◽  
T Cell Repertoire ◽  
Cell Repertoire ◽  
Flow Cytometric

scholarly journals Age-associated decline in T cell repertoire diversity leads to holes in the repertoire and impaired immunity to influenza virus

2008 ◽  
Vol 205 (3) ◽  
pp. 711-723 ◽  
Author(s):  
Eric J. Yager ◽  
Mushtaq Ahmed ◽  
Kathleen Lanzer ◽  
Troy D. Randall ◽  
David L. Woodland ◽  
...  
Keyword(s):  
Influenza Virus ◽  
T Cell ◽  
De Novo ◽  
Cd8 T Cell ◽  
Infection Model ◽  
T Cell Repertoire ◽  
Cell Repertoire ◽  
Aged Mice ◽  
Cell Immunity ◽  
Repertoire Diversity

A diverse T cell repertoire is essential for a vigorous immune response to new infections, and decreasing repertoire diversity has been implicated in the age-associated decline in CD8 T cell immunity. In this study, using the well-characterized mouse influenza virus model, we show that although comparable numbers of CD8 T cells are elicited in the lung and lung airways of young and aged mice after de novo infection, a majority of aged mice exhibit profound shifts in epitope immunodominance and restricted diversity in the TCR repertoire of responding cells. A preferential decline in reactivity to viral epitopes with a low naive precursor frequency was observed, in some cases leading to “holes” in the T cell repertoire. These effects were also seen in young thymectomized mice, consistent with the role of the thymus in maintaining naive repertoire diversity. Furthermore, a decline in repertoire diversity generally correlated with impaired responses to heterosubtypic challenge. This study formally demonstrates in a mouse infection model that naturally occurring contraction of the naive T cell repertoire can result in impaired CD8 T cell responses to known immunodominant epitopes and decline in heterosubtypic immunity. These observations have important implications for the design of vaccine strategies for the elderly.

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