Somatic cells of goat and sheep milk: Analytical, sanitary, productive and technological aspects

2007 ◽  
Vol 68 (1-2) ◽  
pp. 126-144 ◽  
Author(s):  
K. Raynal-Ljutovac ◽  
A. Pirisi ◽  
R. de Crémoux ◽  
C. Gonzalo
Keyword(s):  
Somatic Cells ◽  
Sheep Milk

scholarly journals Quality and safety assessment of sheep milk and colostrum

2020 ◽  
Vol 8 (3) ◽  
pp. 202-205
Author(s):  
N. M. Zazharska ◽  
K. H. Kibets
Keyword(s):  
Electrical Conductivity ◽  
Physicochemical Parameters ◽  
Safety Assessment ◽  
Somatic Cells ◽  
Maximum Amount ◽  
Quality And Safety ◽  
Sheep Milk ◽  
Ewe's Milk ◽  
Breed Sheep ◽  
Organoleptic Parameters

Sheep’s milk contains a significant amount of protein, iron, calcium, vitamins A, B and E, as well as phosphorus and magnesium, compared to cow’s milk. The purpose of research was to identify changes in physicochemical parameters, concentrations of immunoglobulins in sheep’s milk and colostrum depending on the day of lactation. The material of the study was 73 samples of ewes milk and colostrum of the Merinolandschaf breed sheep at the private enterprise «Ukrsilgosprom» in Dnipropetrovsk region. It was found that, in terms of organoleptic parameters, the samples of sheep colostrum of the first days after lambing differed significantly from the milk of the subsequent days of lactation. The color of colostrum in the first days was creamy yellow, in all other samples it varied from white to yellowish; smell – pleasant, specific to sheep’s milk, without foreign odors. The taste of colostrum was salty. The consistency of colostrum for the first three days was viscous, especially on the first day, on the following days, it was homogeneous, without mucus, not viscous. It was found that the acidity of sheep colostrum (1–7 days of lactation) ranged from 13.8°T to 34.0°T, density – from 23.2 °A to 94.1 °A. On the second day of ewes lactation, the number of somatic cells (706 ± 221×103 cells / ml) and immunoglobulins (18.72 ± 5.19 mg/ml) decreased by almost half compared with the first day (1 293 ± 231×103 cells / ml and 37.79 ± 7.45 mg/ml, respectively). Electrical conductivity was the only indicator that increased 1.5 times on the second day of lactation (4.23 ± 0.29 mS/m). On the fifth day of lactation, fat indicators (7.13 ± 0.83 %), density (34.90 ± 1.82 °A), acidity (16.7 ± 0.8 °T) almost halved in comparison with characteristics of the first-day colostrum. On the first day after lambing, the highest acidity was observed (33.9 ± 5.8 °T), which was caused by the maximum amount of immunoglobulins (37.79 ± 7.45 mg/ml) in colostrum. After lambing the number of immunoglobulins significantly decreased, on the 6th day – 4.92 ± 1.88 mg/ml (P < 0.001 compared with the first day). The obtained results allow producers of sheep’s milk and cheese to reduce the withstand of the colostrum period and to mix a secret with collected ewe’s milk on the 5th day of lactation.

scholarly journals Quality of Mountain Sheep Milk Used for the Production of Traditional Cheeses

2020 ◽  
Vol 20 (1) ◽  
pp. 299-314 ◽  
Author(s):  
Aldona Kawęcka ◽  
Marta Pasternak ◽  
Danuta Słoniewska ◽  
Anna Miksza-Cybulska ◽  
Emilia Bagnicka
Keyword(s):  
Somatic Cell ◽  
Somatic Cells ◽  
Fat Content ◽  
Cell Content ◽  
Traditional System ◽  
Sheep Milk ◽  
Urea Content ◽  
Traditional Cheeses ◽  
Quality Of Milk

AbstractThe aim of the research was to assess the quality of milk from mountain sheep used for the production of traditional cheeses, taking into account the influence of the breed, the month of milking, and the content of somatic cells. Milk for the study was obtained from sheep of three mountain breeds: Podhale Zackel (PZ), Polish Mountain Sheep (PMS), and Coloured Mountain Sheep (CMS). The sheep were grazed in mountain pastures after lamb weaning, in the period from May to October in the traditional system. No influence of the breed on the examined parameters was found, except for urea content. Mountain sheep milk was characterized by a content of 19.68% solids, 8.48% fat, 6.63% protein, in which almost 76% was formed by casein (4.99%), and the average lactose content was 4.15%. Other milk parameters also did not differ between breeds: density was 1034.04 g/L, acidity 11.34°SH, and mean somatic cell content was 982.13∙103∙ml−1 (log10SCC = 5.68). The highest urea content was recorded in the milk of Coloured Mountain Sheep (280.69 mg/L) and the lowest urea content was recorded in the milk of Zackel sheep (200.97 mg/L). The month of milking influenced the content of most milk components, but no changes in SCC content during lactation were found. Significant correlations between fat content and other milk parameters were recorded. In the case of urea content, negative, statistically significant correlations with the majority of examined parameters were found.

scholarly journals Gelsolin expression in sheep milk somatic cells during lactation

animal ◽  
2019 ◽  
Vol 13 (10) ◽  
pp. 2297-2304
Author(s):  
F. Napolitano ◽  
F. Grandoni ◽  
F. Signorelli ◽  
G. Annicchiarico ◽  
G. Catillo ◽  
...  
Keyword(s):  
Somatic Cells ◽  
Sheep Milk ◽  
Milk Somatic Cells

An approach for the estimation of somatic cells’ effect in Sarda sheep milk based on the analysis of milk traits and coagulation properties

2019 ◽  
Vol 171 ◽  
pp. 77-81 ◽  
Author(s):  
Pietro Paschino ◽  
Giuseppe Massimo Vacca ◽  
Maria Luisa Dettori ◽  
Michele Pazzola
Keyword(s):  
Somatic Cells ◽  
Milk Traits ◽  
Sheep Milk

Isolation, culture and characterisation of somatic cells derived from semen and milk of endangered sheep and eland antelope

2007 ◽  
Vol 19 (4) ◽  
pp. 576 ◽  
Author(s):  
L. Nel-Themaat ◽  
M. C. Gómez ◽  
P. Damiani ◽  
G. Wirtu ◽  
B. L. Dresser ◽  
...  
Keyword(s):  
Nuclear Transfer ◽  
Cell Attachment ◽  
Gulf Coast ◽  
Somatic Cells ◽  
Cell Types ◽  
Ovis Aries ◽  
Sheep Milk ◽  
Immunocytochemical Detection ◽  
Culture Surface ◽  
Semen Donor

Semen and milk are potential sources of somatic cells for genome banks. In the present study, we cultured and characterised cells from: (1) cooled sheep milk; (2) fresh, cooled and frozen–thawed semen from Gulf Coast native (GCN) sheep (Ovis aries); and (3) fresh eland (Taurotragus oryx) semen. Cells attached to the culture surface from fresh (29%), cooled (43%) and slow-frozen (1°C/min; 14%) ram semen, whereas no attachment occurred in the fast-frozen (10°C/min) group. Proliferation occurred in fresh (50%) and cooled (100%) groups, but no cells proliferated after passage 1 (P1). Eland semen yielded cell lines (100%) that were cryopreserved at P1. In samples from GCN and cross-bred milk, cell attachment (83% and 95%, respectively) and proliferation (60% and 37%, respectively) were observed. Immunocytochemical detection of cytokeratin indicated an epithelial origin of semen-derived cells, whereas milk yielded either fibroblasts, epithelial or a mixture of cell types. Deoxyribonucleic acid microsatellite analysis using cattle-derived markers confirmed that eland cells were from the semen donor. Eland epithelial cells were transferred into eland oocytes and 12 (71%), six (35%) and two (12%) embryos cleaved and developed to morulae or blastocyst stages, respectively. In conclusion, we have developed a technique for obtaining somatic cells from semen. We have also demonstrated that semen-derived cells can serve as karyoplast donors for nuclear transfer.

Effects of nutrition on the contents of fat, protein, somatic cells, aromatic compounds, and undesirable substances in sheep milk

2006 ◽  
Vol 131 (3-4) ◽  
pp. 255-291 ◽  
Author(s):  
Giuseppe Pulina ◽  
Anna Nudda ◽  
Gianni Battacone ◽  
Antonello Cannas
Keyword(s):  
Aromatic Compounds ◽  
Somatic Cells ◽  
Sheep Milk

3-D examination of the cytoskeletal sheets of mammalian eggs

1992 ◽  
Vol 50 (1) ◽  
pp. 914-915
Author(s):  
Carolyn A. Larabell ◽  
David G. Capco ◽  
G. Ian Gallicano ◽  
Robert W. McGaughey ◽  
Karsten Dierksen ◽  
...  
Keyword(s):  
Actin Filaments ◽  
Somatic Cells ◽  
Freeze Fracture ◽  
Blastocyst Stage ◽  
Cell Cytoplasm ◽  
Planar Structures ◽  
Cytoskeletal Network ◽  
Entire Cell ◽  
Cytoskeletal Networks ◽  
Mammalian Eggs

Mammalian eggs and embryos contain an elaborate cytoskeletal network of “sheets” which are distributed throughout the entire cell cytoplasm. Cytoskeletal sheets are long, planar structures unlike the cytoskeletal networks typical of somatic cells (actin filaments, microtubules, and intermediate filaments), which are filamentous. These sheets are not found in mammalian somatic cells nor are they found in nonmammalian eggs or embryos. Evidence that they are, indeed, cytoskeletal in nature is derived from studies demonstrating that 1) the sheets are retained in the detergent-resistant cytoskeleton fraction; 2) there are no associated membranes (determined by freeze-fracture); and 3) the sheets dissociate into filaments at the blastocyst stage of embryogenesis. Embedment-free sections of hamster eggs viewed at 60 kV show sheets running across the egg cytoplasm (Fig. 1). Although this approach provides excellent global views of the sheets and their reorganization during development, the mechanism of image formation for embedment-free sections does not permit evaluation of the sheets at high resolution.

The capacity to reprogramme is an intrinsic property of adult somatic cells

2014 ◽  
Author(s):  
Domenico Iuso ◽  
Paola Toschi ◽  
Debora Agata Anzalone ◽  
Marta Czernik ◽  
Grazyna Ewa Ptak ◽  
...  
Keyword(s):  
Somatic Cells ◽  
Intrinsic Property
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