Human pIgR mimetic peptidic ligand for affinity purification of IgM Part II: Ligand binding characteristics

2013 ◽  
Vol 102 ◽  
pp. 43-49 ◽  
Author(s):  
Satyen Gautam ◽  
Kai-Chee Loh
Keyword(s):  
Ligand Binding ◽  
Affinity Purification ◽  
Binding Characteristics

scholarly journals Mutation of the important Tyr-33 residue of chicken avidin: functional and structural consequences

2003 ◽  
Vol 369 (2) ◽  
pp. 249-254 ◽  
Author(s):  
Ari T. MARTTILA ◽  
Vesa P. HYTÖNEN ◽  
Olli H. LAITINEN ◽  
Edward A. BAYER ◽  
Meir WILCHEK ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Quaternary Structure ◽  
Affinity Purification ◽  
Ph Dependence ◽  
Tight Binding ◽  
Carbonyl Oxygen ◽  
Binding Characteristics ◽  
Biotin Binding ◽  
High Ligand

The strong interaction between avidin and biotin is so tight (dissociation constant 10-15M) that conditions usually sufficient for protein denaturing fail to dislodge biotin from the avidin—biotin complex. This kind of irreversible binding hinders the use of avidin in applications such as affinity purification or protein immobilization. To address this concern, we have constructed a series of mutants of the strategically positioned Tyr-33 in order to study the role of this residue in biotin binding, and to create avidin variants with more reversible ligand-binding properties. Unexpectedly, an avidin mutant in which Tyr-33 was replaced with phenylalanine (Avm-Y33F) displayed similar biotin-binding characteristics to the native avidin, indicating that the hydrogen bond formed between the hydroxy group of Tyr-33 and the carbonyl oxygen of biotin is not as important for the tight binding of biotin as previously suggested. In terms of the reversibility of biotin binding, Avm-Y33H was the most successful substitution constructed in this study. Interestingly, the binding of this mutant exhibited clear pH-dependence, since at neutral pH it bound to the biotin surface in an irreversible fashion, whereas, at pH9, 50% of the bound protein could be released with free biotin. Furthermore, although Tyr-33 is located relatively distant from the monomer—monomer interfaces, the mutagenesis of this residue also weakened the quaternary structure of avidin, indicating that the high ligand binding and the high stability of avidin have evolved together and it is difficult to modify one without affecting the other.

scholarly journals Diversity in the structures and ligand-binding sites of nematode fatty acid and retinol-binding proteins revealed by Na-FAR-1 from Necator americanus

2015 ◽  
Vol 471 (3) ◽  
pp. 403-414 ◽  
Author(s):  
M. Florencia Rey-Burusco ◽  
Marina Ibáñez-Shimabukuro ◽  
Mads Gabrielsen ◽  
Gisela R. Franchini ◽  
Andrew J. Roe ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Ligand Binding ◽  
Tissue Distribution ◽  
Binding Sites ◽  
Binding Proteins ◽  
Retinol Binding Protein ◽  
Internal Cavity ◽  
Binding Characteristics ◽  
Necator Americanus ◽  
Ligand Binding Sites

Necator americanus fatty acid and retinol-binding protein-1 (Na-FAR-1) is an abundantly expressed FAR from a parasitic hookworm. The present work describes its tissue distribution, structure and ligand-binding characteristics and shows that Na-FAR-1 expands to transport multiple FA molecules in its internal cavity.

scholarly journals Binding characteristics of a panel of monoclonal antibodies against the ligand binding domain of the human LDLr

2006 ◽  
Vol 47 (7) ◽  
pp. 1399-1405 ◽  
Author(s):  
Anh T. Nguyen ◽  
Tomoko Hirama ◽  
Vinita Chauhan ◽  
Roger MacKenzie ◽  
Ross Milne
Keyword(s):  
Monoclonal Antibodies ◽  
Ligand Binding ◽  
Binding Domain ◽  
Ligand Binding Domain ◽  
Binding Characteristics

Analysis of idiotypic and anti-idiotypic antibodies as models of receptor and ligand.

1988 ◽  
Vol 34 (9) ◽  
pp. 1676-1680 ◽  
Author(s):  
D S Linthicum ◽  
M B Bolger ◽  
P H Kussie ◽  
G M Albright ◽  
T A Linton ◽  
...  
Keyword(s):  
Molecular Modeling ◽  
Ligand Binding ◽  
Binding Sites ◽  
Molecular Mimicry ◽  
Quantitative Structure ◽  
Binding Characteristics ◽  
Structure Activity ◽  
Bioactive Ligands ◽  
Quantitative Structure Activity Relationships ◽  
Genomic Basis

Abstract Antibodies to small bioactive ligands and peptides may mimic the binding characteristics of the natural receptor; in turn, the anti-idiotypic antibodies generated against the binding sites of such anti-ligand antibodies may mimic some aspects of small bioactive ligands and peptides. Among the several levels of investigation of such antibody-receptor networks are (a) the quantitative structure-activity relationships of ligand binding to antibody as compared with natural receptor; (b) the molecular modeling of antibody-receptor binding sites and the genomic basis for such structures; and (c) the characteristics of the molecular mimicry exhibited by "mimetopes" on anti-idiotypic antibodies. To illustrate the analysis encountered at each of these levels, we discuss here antibody and anti-idiotypic systems that are directed to small neuroactive ligands and their receptors.

The ligand binding characteristics of a kinase-defective A/K1018 human insulin receptor expressed in Rat 1 fibroblasts

1990 ◽  
Vol 1053 (2-3) ◽  
pp. 185-188 ◽  
Author(s):  
Hiroshi Maegawa ◽  
Masashi Kobayashi ◽  
Katsuya Egawa ◽  
Donald A. McClain ◽  
Jerrold M. Olefsky ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Insulin Receptor ◽  
Human Insulin ◽  
Binding Characteristics ◽  
Human Insulin Receptor

scholarly journals Ligand binding characteristics of the Ku80 von Willebrand domain

DNA Repair ◽  
2020 ◽  
Vol 85 ◽  
pp. 102739 ◽  
Author(s):  
Kyungmin Kim ◽  
Jungki Min ◽  
Thomas W. Kirby ◽  
Scott A. Gabel ◽  
Lars C. Pedersen ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Binding Characteristics ◽  
Von Willebrand

scholarly journals Ligand Binding Characteristics of CXCR4 Incorporated into Paramagnetic Proteoliposomes

2001 ◽  
Vol 276 (42) ◽  
pp. 38433-38440 ◽  
Author(s):  
Gregory J. Babcock ◽  
Tajib Mirzabekov ◽  
Woj Wojtowicz ◽  
Joseph Sodroski
Keyword(s):  
Ligand Binding ◽  
Binding Characteristics
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