The acid phosphatase test two minute cut-off: An insufficient time to detect some semen stains

2013 ◽  
Vol 53 (2) ◽  
pp. 187-191 ◽  
Author(s):  
Paul Redhead ◽  
Melanie K. Brown
Keyword(s):  
Acid Phosphatase ◽  
Insufficient Time ◽  
Acid Phosphatase Test

Detection of Human seminal stains in one minute by modified acid phosphatase test

2015 ◽  
Vol 2 (3) ◽  
pp. 14 ◽  
Author(s):  
Bhoopendra Singh ◽  
Ila Gautam ◽  
Vijay Yadav ◽  
Braja Mohapatra
Keyword(s):  
Acid Phosphatase ◽  
Seminal Stains ◽  
Acid Phosphatase Test

The use of acid phosphatase test papers for DNA profiling

2005 ◽  
Vol 45 (2) ◽  
pp. 97-102 ◽  
Author(s):  
A. Reshef ◽  
M. Barash ◽  
N. Gallili ◽  
A. Michael ◽  
P. Brauner
Keyword(s):  
Acid Phosphatase ◽  
Dna Profiling ◽  
Acid Phosphatase Test

scholarly journals Acid Phosphatase and Zinc Tests Are Effective for Semen Examination and Identification to Prove Intercourse

2020 ◽  
Vol 56 (3) ◽  
pp. 192
Author(s):  
Ahmad Yudianto ◽  
Ariyanto Wibowo ◽  
Indah Nuraini ◽  
Htet Htet Aung
Keyword(s):  
Acid Phosphatase ◽  
Prostate Gland ◽  
False Negative ◽  
Sodium Dodecyl ◽  
High Specificity ◽  
External Factors ◽  
Vaginal Canal ◽  
Acid Phosphatase Test ◽  
Low Sensitivity ◽  
Phosphatase Enzyme

The presence of spermatozoa in vagina is a definite sign of sexual intercourse. However, sometimes microscopic examination does not find spermatozoa or reveals a false negative result. This is influenced by many factors, including the absence of ejaculate in the vaginal canal. In addition, there are other factors such as oligo/azoospermia, vasectomy, degeneration of sperm due to time, incorrect sampling, and improper storage. Therefore, examination of the other components of the ejaculate, ie. the enzyme acid phosphatase, choline and spermin, is important. Compared with spermatozoa, the enzyme phosphatase, choline and spermin have lower evidentiary value because these three components are less specific. However, the level of phosphatase enzyme found in the vagina is much lower than phosphatase enzyme that comes from prostate gland. In this study, as many as 192 samples in the form of patches with sperm/semen stains were tested with acid phosphatase test and zinc test through direct and indirect examination. Washing was carried out using 7 types of detergent for each 4 patch samples, and washing using water as control. The results showed very low sensitivity (0.186) and very high specificity (100%). This showed that both tests had high specificity values. Acid phosphatase test specifically showed the presence of the enzyme phosphatase, while zinc test specifically showed the presence of zinc in semen. This phosphohydrolase-phosphatase enzyme is easily degraded due to external factors, including temperature, humidity, and chemicals, ie. the element SDS (Sodium Dodecyl Sulfate) that has the ability to cut enzymes. The weakness of the acid phosphatase test is that this enzyme is easily degraded, either partially or completely, due to external factors, such as temperature, humidity, heat, and the presence of chemicals.

scholarly journals Acid Phosphatase and Zinc Tests Are Effective for Semen Examination and Identification to Prove Intercourse

2021 ◽  
Vol 56 (3) ◽  
pp. 192
Author(s):  
Ahmad Yudianto ◽  
Ariyanto Wibowo ◽  
Indah Nuraini ◽  
Htet Htet Aung
Keyword(s):  
Acid Phosphatase ◽  
Prostate Gland ◽  
False Negative ◽  
Sodium Dodecyl ◽  
High Specificity ◽  
External Factors ◽  
Vaginal Canal ◽  
Acid Phosphatase Test ◽  
Low Sensitivity ◽  
Phosphatase Enzyme

The presence of spermatozoa in vagina is a definite sign of sexual intercourse. However, sometimes microscopic examination does not find spermatozoa or reveals a false negative result. This is influenced by many factors, including the absence of ejaculate in the vaginal canal. In addition, there are other factors such as oligo/azoospermia, vasectomy, degeneration of sperm due to time, incorrect sampling, and improper storage. Therefore, examination of the other components of the ejaculate, ie. the enzyme acid phosphatase, choline and spermin, is important. Compared with spermatozoa, the enzyme phosphatase, choline and spermin have lower evidentiary value because these three components are less specific. However, the level of phosphatase enzyme found in the vagina is much lower than phosphatase enzyme that comes from prostate gland. In this study, as many as 192 samples in the form of patches with sperm/semen stains were tested with acid phosphatase test and zinc test through direct and indirect examination. In the first method, washing was carried out on day 1, day 7, and month 3, and testing was carried out after each washing. In the second method, washing was carried out simultaneously and testing was carried out on day 1, day 7 and month 3. Washing was carried out using 7 types of detergent for each 4 patch samples, and washing using water as control. The results showed very low sensitivity (0.186) and very high specificity (100%). This showed that both tests had high specificity values. Acid phosphatase test specifically showed the presence of the enzyme phosphatase, while zinc test specifically showed the presence of zinc in semen. This phosphohydrolase-phosphatase enzyme is easily degraded due to external factors, including temperature, humidity, and chemicals, ie. the element SDS (Sodium Dodecyl Sulfate) that has the ability to cut enzymes. The weakness of the acid phosphatase test is that this enzyme is easily degraded, either partially or completely, due to external factors, such as temperature, humidity, heat, and the presence of chemicals.

scholarly journals Semen Stain Detection: A Study on the Different Fabrics for Effects of Washing

2020 ◽  
Vol 5 (4) ◽  
Author(s):  
Bharti Jain
Keyword(s):  
Acid Phosphatase ◽  
Light Source ◽  
Seminal Fluid ◽  
Acid Phosphatase Test ◽  
Day By Day

As the number of rape cases are increasing day by day. There is a requirement of detection of seminal fluid and sperm on various surfaces, even if semen and seminal fluid available in few quantities. The present work focuses to determine three possible aspects affecting semen determination on laundered clothing: the temperature of water while laundering and handling if the stain was dry or wet at the period of laundering of the fabric form. During present work, four types of fabrics (Polyester, Denim, Cotton and wool) were analysed microscopically by applying of methods to determine semen (U.V. light source, Acid phosphatase test) the outcome demonstrates the significance of the methodology and precautions to be taken whenever evaluating findings for the identification of semen, in general on objects which may have been laundered.

Sign in / Sign up

Export Citation Format

Share Document