Leaf-color mutation induced by ethyl methane sulfonate and genetic and physio-biochemical characterization of leaf-color mutants in pepper (Capsicum annuum L.)

2019 ◽  
Vol 257 ◽  
pp. 108709
Author(s):  
Guo-Xin Cheng ◽  
Rui-Xing Zhang ◽  
Shuai Liu ◽  
Yu-Mei He ◽  
Xiao-Hui Feng ◽  
...  
Keyword(s):  
Capsicum Annuum ◽  
Biochemical Characterization ◽  
Ethyl Methane Sulfonate ◽  
Leaf Color ◽  
Methane Sulfonate ◽  
Ethyl Methane ◽  
Capsicum Annuum L ◽  
Color Mutation

scholarly journals Development and Characterization of an Ethyl Methane Sulfonate (EMS) Induced Mutant Population in Capsicum annuum L.

Plants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 396
Author(s):  
Muhammad Irfan Siddique ◽  
Seungki Back ◽  
Joung-Ho Lee ◽  
Jinkwan Jo ◽  
Siyoung Jang ◽  
...  
Keyword(s):  
Capsicum Annuum ◽  
Reverse Genetics ◽  
Growth Habit ◽  
Ethyl Methane Sulfonate ◽  
Induced Mutations ◽  
Methane Sulfonate ◽  
Ethyl Methane ◽  
Mutant Population ◽  
Knock Out ◽  
Plant Growth Habit

Plant breeding explores genetic diversity in useful traits to develop new, high-yielding, and improved cultivars. Ethyl methane sulfonate (EMS) is a chemical widely used to induce mutations at loci that regulate economically essential traits. Additionally, it can knock out genes, facilitating efforts to elucidate gene functions through the analysis of mutant phenotypes. Here, we developed a mutant population using the small and pungent ornamental Capsicum annuum pepper “Micro-Pep”. This accession is particularly suitable for mutation studies and molecular research due to its compact growth habit and small size. We treated 9500 seeds with 1.3% EMS and harvested 3996 M2 lines. We then selected 1300 (32.5%) independent M2 families and evaluated their phenotypes over four years. The mutants displayed phenotypic variations in plant growth, habit, leaf color and shape, and flower and fruit morphology. An experiment to optimize Targeting Induced Local Lesions IN Genomes (TILLING) in pepper detected nine EMS-induced mutations in the eIF4E gene. The M2 families developed here exhibited broad phenotypic variation and should be valuable genetic resources for functional gene analysis in pepper molecular breeding programs using reverse genetics tools, including TILLING.

scholarly journals A step towards Balkan Capsicum annuum L. core collection: Phenotypic and biochemical characterization of 180 accessions for agronomic, fruit quality, and virus resistance traits

PLoS ONE ◽  
2020 ◽  
Vol 15 (8) ◽  
pp. e0237741
Author(s):  
Amol N. Nankar ◽  
Velichka Todorova ◽  
Ivanka Tringovska ◽  
Gancho Pasev ◽  
Vesela Radeva-Ivanova ◽  
...  
Keyword(s):  
Capsicum Annuum ◽  
Fruit Quality ◽  
Virus Resistance ◽  
Core Collection ◽  
Biochemical Characterization ◽  
Capsicum Annuum L ◽  
Resistance Traits

PERLAKUAN ETHYL METHANE SULFONATE (EMS) PADA ENTEROBACTER AEROGENES AY-2 DARI LIMBAH METAN FERMENTASI UNTUK PENINGKATAN PRODUKSI GAS HIDROGEN

2018 ◽  
Vol 5 (1) ◽  
Author(s):  
Mahyudin Abdul Rachman
Keyword(s):  
Metabolic Pathway ◽  
Acid Production ◽  
Double Mutant ◽  
Enterobacter Aerogenes ◽  
H2 Production ◽  
Hydrogen Gas ◽  
Survival Ratio ◽  
Ethyl Methane Sulfonate ◽  
Methane Sulfonate ◽  
Ethyl Methane

Enterobacter aerogenes AY-2 mutant is known for hydrogen gas producer which ws obtained from the sludge of methane fermentation and the yield is 1.5 fold higher than wildtype. Hydrogen gas production can be gain via NADH oxidation in anaerobic metabolic pathway by blocking organic acid production. Metabolic pathway can be changed by mutagenesis. Enterobacter aerogenes AY-2 mutated with ethyl methane sulfonate in logarithmic phase with consentration 10, 11, 12, 13, 14 and 15 μl/ml cell suspention during 120 minute. Mutation that result lowest survival ratio (0,01%) was 14 μl EMS/ml cell suspention is repeated with variation incubation time, 30, 60, 90 and 120 minute. 166 double mutant colony has been collected and choosen randomly. The choosen 43 colony was fermented in glycerol complex medium for determining ten double mutant with the highest H2 production. Double mutant AD-H43 is a highest H2 producer that increase 20% H2 production from AY-2 and has a decrease lactid acid production, 31% less from AY-2. Increasing H2 production in double mutant AD-H43 is caused by lactate dehydrogenase deffi cient.Keywords: Enterobacter aerogenes AY-2, ethyl methane sulfonate (EMS), H2 and methane sludge

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