Genetic diversity, germplasm identification and population structure of Diospyros kaki Thunb. from different geographic regions in China using SSR markers

2019 ◽  
Vol 251 ◽  
pp. 233-240 ◽  
Author(s):  
Changfei Guan ◽  
Pingxian Zhang ◽  
Chaoqiong Hu ◽  
Sadaruddin Chachar ◽  
Adeel Riaz ◽  
...  
2020 ◽  
pp. 1032-1041
Author(s):  
Wassu Mohammed ◽  
Beyene Amelework ◽  
Hussein Shimelis

The study was carried out to assess the genetic diversity and population structure of okra collections from diverse geographic origin using selected polymorphic simple sequence repeat (SSR) markers. Thirty-two okra accessions collected from three geographic regions were genotyped using 16 selected SSR markers. The SSR markers generated a total of 71 loci of which 67% were polymorphic. Genetic distances among accessions ranged from 2.2 to 7.1, with a mean of 4.65. Gene diversity ranged from 0.10 to 0.78 with a mean of 0.52. Genetic clustering classified the accessions into three major clusters and four sub-clusters. Each cluster and sub-cluster consisted of accessions derived from different sources. Analysis of molecular variance revealed that 67%, 81% and 83% of the total genetic variation detected was found within populations of geographic origin, altitude and collection district, respectively. The observed moderate to high population differentiation could partly be attributed to limited germplasm exchange, agro-ecological differences, and partly by selection pressure. The present study revealed the presence of high genetic diversity and population divergence among okra collections from Ethiopia. The study demonstrated that a collection strategy for conservation of okra genetic resources should focus on sampling of representative genotypes covering wide geographic regions and altitudinal ranges of target agro-ecologies.


2020 ◽  
Vol 52 (6) ◽  
Author(s):  
Anpei Zhou ◽  
Dan Zong ◽  
Peihua Gan ◽  
Yao Zhang ◽  
Dan Li ◽  
...  

2013 ◽  
Vol 38 (12) ◽  
pp. 2286-2296 ◽  
Author(s):  
Wen-Di YUE ◽  
Li-Bin WEI ◽  
Ti-De ZHANG ◽  
Chun LI ◽  
Hong-Mei MIAO ◽  
...  

2021 ◽  
Author(s):  
Varun Hiremath ◽  
Kanwar Pal Singh ◽  
Neelu Jain ◽  
Kishan Swaroop ◽  
Pradeep Kumar Jain ◽  
...  

Abstract Genetic diversity and structure analysis using molecular markers is necessary for efficient utilization and sustainable management of gladiolus germplasm. Genetic analysis of gladiolus germplasm using SSR markers is largely missing due to scarce genomic information. In the present investigation, we report 66.66% cross transferability of Gladiolus palustris SSRs whereas 48% of Iris EST-SSRs were cross transferable across the gladiolus genotypes used in the study. A total of 17 highly polymorphic SSRs revealed a total 58 polymorphic loci ranging from two to six in each locus with an average of 3.41 alleles per marker. PIC values ranged from 0.11 to 0.71 with an average value of 0.48. Four SSRs were selectively neutral based on Ewens-Watterson test. Analysis of genetic structure of 84 gladiolus genotypes divided whole germplasm into two subpopulations. 35 genotypes were assigned to subpopulation 1 whereas 37 to subpopulation 2 and rest of the genotypes recorded as admixture. Analysis of molecular variance indicated maximum variance (53.59%) among individuals within subpopulations whereas 36.55% of variation observed among individuals within total population. Least variation (9.86%) was noticed between two subpopulations. Moderate (FST = 0.10) genetic differentiation of two subpopulations was observed. Grouping pattern of population structure was consistent with UPGMA dendrogram based on simple matching dissimilarity coefficient (ranged from 01.6 to 0.89) and PCoA. Genetic relationships assessed among the genotypes of respective clusters assist the breeders in selecting desirable parents for crossing. SSR markers from present study can be utilized for cultivar identification, conservation and sustainable utilization of gladiolus genotypes for crop improvement.


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