Alleviating shoot tip necrosis during in vitro propagation of grape cv. Red Globe

2019 ◽  
Vol 248 ◽  
pp. 118-125 ◽  
Author(s):  
N.C. Surakshitha ◽  
K. Soorianathasundaram ◽  
M. Ganga ◽  
M. Raveendran
Keyword(s):  
In Vitro Propagation ◽  
Shoot Tip ◽  
Shoot Tip Necrosis

scholarly journals Effectiveness of calcium chloride in reduction of shoot necrosis incocoa (Theobroma cacao L.) in vitro propagation

2012 ◽  
Vol 28 (1) ◽  
pp. 23-31
Author(s):  
Sulistyani Pancaningtyas
Keyword(s):  
Calcium Chloride ◽  
In Vitro Propagation ◽  
Shoot Growth ◽  
Development Stage ◽  
Shoot Tip ◽  
Maturation Stage ◽  
Randomized Design ◽  
Growth Development ◽  
Shoot Tip Necrosis

Various efforts have been developed for the optimization of the various stages in vitro micropropagation. The maturation stage and pre-acclimatization plantlets is an important stages that must be considered to produce vigorous plants and ready to be planted in the field. The purpose of this study was to reduced shoot tip necrosis in cocoa planlets to obtaine vigorous planlets from in vitro propagation through the addition of Calcium Chloride (CaCl2). The study used two stages of embryogenic development. The first was embryo maturation stage and the second was the shoot growth development stage. The study was arranged factorially in experiment design of Completely Randomized Design consisting of two factors i.e. concentration of CaCl2 consisted of 0, 50, 100, 150, and 200 mg/l and clones consisted of Sulawesi 1 and Sca 6. Each experiment was repeated three times, so the number of combination trials were 5x2x3=30 experimental units. The parameters observed included shoot growth percentage and vigorous planlets percentage. The results showed that the addition of CaCl2 at a concentration of 150 mg/l during maturition stage increased the embryos performance and percentage of shoot tip. However, it could not prevent the shoot tip necrosis. Whereas, the addition at a concentration of 50 mg/l during the shoot m growth development stage could reduced necrosis, suggested to increase the quality of in vitro planlets.Key words: Cocoa, necrosis, calcium chloride, somatic embryogenesis, embryos, planlets, in vitro.

scholarly journals Alleviation of shoot tip necrosis in in vitro propagation of Salvia santolinifolia, Boiss

2020 ◽  
Vol 25 (2) ◽  
pp. 1356-1361
Author(s):  
TOUR JAN ◽  
◽  
ALI HAZRAT ◽  
BEENA NAQVI ◽  
KHAN SHER ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Shoot Tip ◽  
Shoot Tip Necrosis

scholarly journals In vitro Propagation of Solanum capsicoides All. – An Important Therapeutic Agent 'Kantakari'

1970 ◽  
Vol 20 (2) ◽  
pp. 179-184
Author(s):  
N.P. Anish ◽  
M.G. Rajesh ◽  
Jiby Elias ◽  
N. Jayan
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Therapeutic Agent ◽  
Shoot Tip ◽  
Shoot Induction ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants from in vitro germinated seedlings of Solanum capsicoides All. inoculated on MS containing 2 mg/l BA produced maximum shoot induction response (26 shoots per explant). Rooting of the microshoots (19.4 roots per explant) was obtained better in half strength of MS supplemented with NAA (0.5 mg/l). Well rooted plantlets were successfully hardened with 80 per cent survival rate.   Key words: Solanum capsicoides, Propagation, Therapeutic agent   D.O.I. 10.3329/ptcb.v20i2.6912   Plant Tissue Cult. & Biotech. 20(2): 179-184, 2010 (December)

scholarly journals Influence of Ca2+ and 6-benzyladenine on chestnut (Castanea sativa Mill.) in vitro shoot-tip necrosis

Plant Science ◽  
1996 ◽  
Vol 118 (1) ◽  
pp. 89-95 ◽  
Author(s):  
Claudia Piagnani ◽  
Graziano Zocchi ◽  
Ilaria Mignani
Keyword(s):  
Castanea Sativa ◽  
Shoot Tip ◽  
Castanea Sativa Mill ◽  
Shoot Tip Necrosis
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