Sugar accumulation and activities of enzymes involved in fructan dynamics from seedling to bulb formation in onion (Allium cepa L.)

2019 ◽  
Vol 247 ◽  
pp. 147-155 ◽  
Author(s):  
Satoshi Oku ◽  
Keiji Ueno ◽  
Yu Tsuruta ◽  
Yutaka Jitsuyama ◽  
Takashi Suzuki ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Sugar Accumulation ◽  
Bulb Formation ◽  
Allium Cepa L

scholarly journals IDENTIFICATION OF BULBING HORMONE GENES IN ONION (Allium cepa)

2015 ◽  
Vol 2 (1) ◽  
pp. 630
Author(s):  
Minami Tagashira ◽  
Tsuyoshi Kaneta
Keyword(s):  
Allium Cepa ◽  
Expression Analysis ◽  
Higher Plants ◽  
Day Length ◽  
Induction Medium ◽  
Cdna Clones ◽  
Bulb Formation ◽  
Allium Cepa L ◽  
High Efficient ◽  
Shoot Induction Medium

<p>Initiation of onion (Allium cepa L.) bulb formation seems to require “bulbing hormone” which has been considered to be produced in leaf blades in response to the stimulus of long day conditions. But “bulbing hormone” is not yet identified. Previous study revealed a protein called Flowering locusT (FT) as flowering hormone, florigen. FT act for flowering by change on the day length on the higher plants. Objective of this study is identify”bulbing hormone” in onion plants. Method used in this study are cloning gene and gene expression analysis of the FT in onion plants.Full length of cDNA was cloned by the degenerate PCR and 5’­and 3’-RACE method.As a result, six kinds of full length cDNA clones for FThomologs in onion plants were obtained.These genes were named AcFT1 to6. By expression analysis of these genes, AcFT4, 5 and 6,expression increased as it got closer to a condition in long days in association with the bulbing of onion. Furthermore, in order to investigate the functions of these genes, we optimize transformation methods for onion plants. Medium containing 2,4-Dand kinetin showed high efficient plant regeneration from seed-derived callus of onion. Medium containing 2,4-D and kinetin as plant growth regulators is effective for induction of shoot-inducible callus, and advance shoots were developed from the callus on the shoot induction medium which contained thidiazuron, benzyl adenine or trans-zeatin as cytokinins. In conclusion, bulbing hormone in onion plants were possibly gene AcFT4, 5,6.</p><p><br /><strong>Keywords</strong> : Allium cepa L, bulbing hormone gene, Flowering locusT</p>

Цитогенетические эффекты гамма-излучения в проростках Allium cepa L.

2018 ◽  
Vol 481 (1) ◽  
pp. 99-103
Author(s):  
А.Я. Болсуновский ◽  
Д.В. Дементьев ◽  
Е.М. Иняткина ◽  
Ю.В. Кладько ◽  
М.В. Петриченков ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Allium Cepa L
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