Transformation of Mecardonia (Plantaginaceae) with wild-type Agrobacterium rhizogenes efficiently improves compact growth, branching and flower related ornamental traits

2018 ◽  
Vol 234 ◽  
pp. 300-311 ◽  
Author(s):  
Mariana Cecilia Pérez de la Torre ◽  
Paula Fernández ◽  
Julián Alejandro Greppi ◽  
María Andrea Coviella ◽  
Martín Nicolás Fernández ◽  
...  
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Wild Type ◽  
Compact Growth ◽  
Ornamental Traits

scholarly journals The rolB-Like Part of the Agrobacterium rhizogenes orf8 Gene Inhibits Sucrose Export in Tobacco

2002 ◽  
Vol 15 (9) ◽  
pp. 956-962 ◽  
Author(s):  
Marie Umber ◽  
Lars Voll ◽  
Andreas Weber ◽  
Pierre Michler ◽  
Léon Otten
Keyword(s):  
Acetic Acid ◽  
Agrobacterium Rhizogenes ◽  
Mode Of Action ◽  
Sucrose Transporter ◽  
Naphthalene Acetic Acid ◽  
Wild Type ◽  
Sucrose Transport ◽  
Tobacco Plants ◽  
Indole 3 Acetic Acid ◽  
Lines Of Evidence

Many Agrobacterium T-DNA genes belong to the highly diverse rolB family. The mode of action of most of these genes is still unknown. rolB-like sequences also are present at the 5′ ends of the T-DNA-located iaaM genes and the iaaM homolog orf8, whereas iaaM genes from Pseudomonas and Erwinia spp. lack such sequences. iaaMgenes encode tryptophan monooxygenases; these enzymes convert tryptophan into indole-3-acetamide, a precursor of indole-3-acetic acid. Tobacco plants expressing the rolB-like part of the A4 orf8 gene (2×35S-A4-Norf8 plants) accumulate glucose, fructose, sucrose, and starch and resemble sucrose transporter (NtSUT1) antisense plants. Different lines of evidence indicate that 2×35S-A4-Norf8 plants export less sucrose from source leaves. Glucose, fructose, sucrose, and starch accumulate in source leaves during sink-source transition, whereas sink tissues like petioles and midveins contain lower levels than normal. Petiole exudation experiments demonstrate a significant decrease in export of label after 14C-sucrose infiltration and after 14CO2 labeling. Grafting of stunted homozygous 2×35S-A4-Norf8 plants onto wild-type rootstocks restores growth, indicating that unloading is not affected. Growth of 2×35S-A4-Norf8 seedlings is inhibited on naphthalene acetic acid-containing media, suggesting a link between sucrose transport and auxin sensitivity.

Agrobacterium rhizogenes-Mediated Transformation of Hypericum tomentosum L. and Hypericum tetrapterum Fries.

2009 ◽  
Vol 64 (11-12) ◽  
pp. 864-868 ◽  
Author(s):  
Hedviga Komarovská ◽  
Annalisa Giovannini ◽  
Ján Košuth ◽  
Eva Čellárová
Keyword(s):  
Genetic Transformation ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Wild Type ◽  
Root Segments ◽  
Transgenic Lines ◽  
Type Strains ◽  
Hypericum Species

This is the fi rst evidence on successful Agrobacterium rhizogenes-mediated genetic transformation of two species from the genus Hypericum, H. tomentosum L. and H. tetrapterum Fries. Hairy root cultures were induced from root segments of both Hypericum species by two agropine wild-type strains of A. rhizogenes, ATCC 15834 and A4. The transgenic character of the hairy root cultures was proved by PCR amplifi cation of the rolABCD genes. In some H. tetrapterum transgenic lines aux genes were detected as well.

Production of tumours and roots by Ephedra following Agrobacterium rhizogenes infection

1994 ◽  
Vol 72 (2) ◽  
pp. 203-207 ◽  
Author(s):  
Niamh A. O'Dowd ◽  
David H. S. Richardson
Keyword(s):  
Growth Rate ◽  
Plant Growth ◽  
Agrobacterium Rhizogenes ◽  
Dry Weight ◽  
Wild Type ◽  
Bacterial Strains ◽  
Heat Treated ◽  
Slow Growing

This paper contains the first report that stems of the Gnetophyte Ephedra respond to infection by Agrobacterium rhizogenes by producing roots and tumours in vivo and in vitro. Of the bacterial strains employed, the wild-type Ar2629 gave the maximum response, and strain LBA9402 was also effective. In no case did heat-treated A. rhizogenes produce tumours or roots. Excised tumour tissues were cultured for more than 2 years in the absence of exogenous plant-growth regulators without any deterioration in growth rate. In vivo tumours of Ephedra fragilis and Ephedra minima contained up to 0.3% dry weight l-ephedrine, and slow-growing in vitro cultured tumours of E. fragilis contained up to 0.01% l-ephedrine, but alkaloid was not detected in faster growing isolates. Key words: Agrobacterium rhizogenes, alkaloid, Ephedra, l-ephedrine, Gnetophytes, gymnosperm, tumours.

scholarly journals The T-DNA Oncogene A4-orf8 from Agrobacterium rhizogenes A4 Induces Abnormal Growth in Tobacco

2005 ◽  
Vol 18 (3) ◽  
pp. 205-211 ◽  
Author(s):  
Marie Umber ◽  
Bernadette Clément ◽  
Léon Otten
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Soluble Sugars ◽  
Starch Accumulation ◽  
Wild Type ◽  
Abnormal Growth ◽  
Regenerated Plants ◽  
Physical Linkage ◽  
Dark Green ◽  
Characteristic Growth ◽  
Fad Binding

The related orf8 and iaaM T-DNA genes from Agrobacterium are each composed of two distinct parts. The 5′ parts (called Norf8 or NiaaM) encode a 200-amino-acid (aa) sequence with homology to various T-DNA oncoproteins such as RolB, RolC, and 6b. The 3′ parts (Corf8 or CiaaM) encode a 550-aa sequence with homology to IaaM proteins from Pseudomonas and Pantoea spp. Whereas iaaM genes encode flavin adenine dinucleotide (FAD)-dependent tryptophan 2-monooxygenases that catalyze the synthesis of indole-3-acetamide (IAM), A4-orf8 from Agrobacterium rhizogenes A4 does not. Plants expressing a 2x35S-A4-Norf8 construct accumulate soluble sugars and starch. We now have regenerated plants that express the full-size 2x35S-A4-orf8 and the truncated 2x35S-A4-Corf8 gene. 2x35S-A4-Corf8 plants accumulate starch and show reduced growth like 2x35SA4-Norf8 plants but, in addition, display a novel set of characteristic growth modifications. These consist of leaf hypertrophy and hyperplasia (blisters); thick, dark-green leaves; thick stems; and swollen midveins. Mutations in the putative FAD-binding site of A4-Orf8 did not affect the blister syndrome. Plants expressing 2x35S-A4-Corf8 had a normal phenotype but contained less starch and soluble sugars than did wild-type plants. When 2x35S-A4-Corf8 plants were crossed to starch-accumulating 2x35S-A4-Norf8 plants with reduced growth, A4-Corf8 partially restored growth and reduced starch accumulation. A4-Corf8xA4-Norf8 crosses did not lead to the blister syndrome, suggesting that this requires physical linkage of the A4-NOrf8 and A4-COrf8 sequences.

scholarly journals Draft Genome Sequence of Agrobacterium rhizogenes Strain NCPPB2659

2016 ◽  
Vol 4 (4) ◽  
Author(s):  
Jose A. Valdes Franco ◽  
Ray Collier ◽  
Yi Wang ◽  
Naxin Huo ◽  
Yong Gu ◽  
...  
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Genome Sequence ◽  
Hairy Root ◽  
Wild Type Strain ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Virulence Plasmid ◽  
Wild Type ◽  
Circular Chromosome ◽  
Transgenic Roots

This work reports the draft genome sequence of Agrobacterium rhizogenes strain NCPPB2659 (also known as strain K599). The assembled genome contains 5,277,347 bp, composed of one circular chromosome, the pRi2659 virulence plasmid, and 17 scaffolds pertaining to the linear chromosome. The wild-type strain causes hairy root disease in dicots and has been used to make transgenic hairy root cultures and composite plants (nontransgenic shoots with transgenic roots). Disarmed variants of the strain have been used to produce stable transgenic monocot and dicot plants.

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